GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE10251 Query DataSets for GSE10251
Status Public on Jan 17, 2009
Title Gene expression profiling implicates novel hormonal regulation of the floral initiation process in soybeans
Organism Glycine max
Experiment type Expression profiling by array
Summary The transition to flowering is characterized by a shift of the shoot apical meristem (SAM) from leaf production to the initiation of a floral meristem. In this study, we addressed the nature of SAM gene networks involved in the early floral initiation process in the crop legume soybean. Unique aspects (such as pod development and nitrogen fixation) of legume development make them appealing for plant development studies. Soybean, a major oilseed crop, possesses varied maturity groups; hence, understanding and unravelling initial transition control has implications in manipulating crop yield. To this end, we performed global gene expression analysis using Affymetrix® soybean GeneChip® with RNA isolated from micro-dissected soybean SAMs at various time points after plants were shifted from long-day to short-day growth conditions. Analysis of the resulting microarray data revealed a total of 331 transcripts that have differential expression profiles. Intriguingly, about 20% of the transcripts affected by the switch in the development program have orthologs reported to be responsive to abscisic acid (ABA), suggesting an increase in ABA levels in the SAM during this developmental change. A subsequent immunoassay verified this, thereby implicating its possible function as an endogenous signal during the floral evocation process. The striking occurrence of abiotic stress-related transcripts, including trehalose metabolism genes, in SAMs during the early transition to floral meristems points to an overlap of abiotic stress and floral signalling pathways in soybean. In addition, other hormones - auxin, jasmonic acid and brassinosteroids - and a number of candidate protein kinases may also act in the signalling process prior to or concurrently with the induction of the putative floral homeiotic transcripts. This indicates that molecular events mediated by multiple hormonal pathways are part of the mechanism employed by soybean to regulate the floral transition process.
Keywords: transcript profiling floral transition soybean shoot apical meristem
Overall design SAMs were micro-dissected from soybean plants under the dissecting microscope at 40x magnification. Any leaf primordia were excluded in order to create a meristem-enriched tissue collection. Approximately 70 SAMs were dissected for each time point (0 SD, 1 SD, 2 SD, 4 SD and 6 SD). Two independent biological replicates were incorporated.
Contributor(s) Wong CE, Singh MB, Bhalla PL
Citation(s) 18980639
Submission date Jan 23, 2008
Last update date Nov 22, 2016
Contact name Chui E WONG
Organization name University of Melbourne
Department Land and Food
Lab Plant Molecular Biology and Biotechnology Group
Street address University of Melbourne
City Parkville
State/province Victoria
ZIP/Postal code 3010
Country Australia
Platforms (1)
GPL4592 [Soybean] Affymetrix Soybean Genome Array
Samples (10)
GSM258496 Vegetative soybean shoot apical meristem
GSM258497 Soybean Shoot Apical Meristem, 1 short-day treatment
GSM258498 Soybean shoot apical meristem, 2 short-day treatment
BioProject PRJNA108439

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10251_RAW.tar 54.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap