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Status
Public on Feb 02, 2007
Title
Tsinghua University mammalian 2K microRNA microarray
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organisms
Homo sapiens ; Mus musculus ; Rattus norvegicus Manufacturer
CapitalBio
Manufacture protocol
as described in CapitalBio lab manual
Support
glass
Coating
unknown
Description
Altogether 509 mature miRNA sequences were assembled and integrated into our miRNA microarray design, these comprised 435 human (including a further 122 predicted miRNA sequences from published references (Xie et al., Nature 2005,434:338-345 ), 196 rat and 261 mouse mature miRNAs from the miRNA Registry (Sanger, accessed Sept. 2005). In addition, we designed 8 short oligonucleotides that possessed no homology to any known RNA sequence and we produced their corresponding synthetic miRNAs by in vitro transcription using Ambion's miRNA Probe Construction kit (Cat. No.1550, Austin, TX). We added various amounts of these synthetic miRNAs into the human miRNA samples prior to analysis as internal controls.
Contributor(s)
Guo Y Submission date
Oct 30, 2006
Last update date
Feb 02, 2007
Contact name
yong guo
E-mail(s)
yongguo@tsinghua.edu.cn Phone
(86)-10-62796071
Fax
(86)-10-62773059
Organization name
Tsinghua University
Department
Medical Systems Biology Research Center
Street address
Qinghuayuan
City
Beijing
State/province
Beijing
ZIP/Postal code
100084
Country
China
Samples (263)
GSM143030 , GSM143031 , GSM143032 , GSM143033 , GSM143034 , GSM143036
GSM143037 ,
GSM143039 ,
GSM143041 ,
GSM143043 ,
GSM143049 ,
GSM143051 ,
GSM143053 ,
GSM143055 ,
GSM143060 ,
GSM143061 ,
GSM143062 ,
GSM143063 ,
GSM143064 ,
GSM143065 ,
GSM143066 ,
GSM143067 ,
GSM143068 ,
GSM143069 ,
GSM143070 ,
GSM143072 ,
GSM143074 ,
GSM143075 ,
GSM143078 ,
GSM143080 ,
GSM143086 ,
GSM143087 ,
GSM143090 ,
GSM143091 ,
GSM143092 ,
GSM143093 ,
GSM143094 ,
GSM143095 ,
GSM143099 ,
GSM143100 ,
GSM143102 ,
GSM143103 ,
GSM143104 ,
GSM143105 ,
GSM143106 ,
GSM143107 ,
GSM143108 ,
GSM143109 ,
GSM143111 ,
GSM143112 ,
GSM143113 ,
GSM143114 ,
GSM143115 ,
GSM143116 ,
GSM143117 ,
GSM143118 ,
GSM143167 ,
GSM143168 ,
GSM143169 ,
GSM143170 ,
GSM143174 ,
GSM143175 ,
GSM143176 ,
GSM143177 ,
GSM143179 ,
GSM143180 ,
GSM143182 ,
GSM143183 ,
GSM143185 ,
GSM143186 ,
GSM143188 ,
GSM143189 ,
GSM143190 ,
GSM143191 ,
GSM143193 ,
GSM143194 ,
GSM143195 ,
GSM143196 ,
GSM143197 ,
GSM143199 ,
GSM143200 ,
GSM143201 ,
GSM143203 ,
GSM143204 ,
GSM143206 ,
GSM143207 ,
GSM143208 ,
GSM143209 ,
GSM143211 ,
GSM143212 ,
GSM143213 ,
GSM143214 ,
GSM143215 ,
GSM143216 ,
GSM143218 ,
GSM143219 ,
GSM143220 ,
GSM143221 ,
GSM143223 ,
GSM143224 ,
GSM143226 ,
GSM143227 ,
GSM143229 ,
GSM143230 ,
GSM143231 ,
GSM143232 ,
GSM143233 ,
GSM143234 ,
GSM143235 ,
GSM143236 ,
GSM143237 ,
GSM143238 ,
GSM143239 ,
GSM143240 ,
GSM143241 ,
GSM143242 ,
GSM143244 ,
GSM143245 ,
GSM143246 ,
GSM143261 ,
GSM143262 ,
GSM143263 ,
GSM143264 ,
GSM143265 ,
GSM143266 ,
GSM143267 ,
GSM143268 ,
GSM143269 ,
GSM143270 ,
GSM143271 ,
GSM143272 ,
GSM143273 ,
GSM143274 ,
GSM143275 ,
GSM143276 ,
GSM143277 ,
GSM143278 ,
GSM143279 ,
GSM143280 ,
GSM254540 ,
GSM254541 ,
GSM254542 ,
GSM254543 ,
GSM254544 ,
GSM254545 ,
GSM254546 ,
GSM254547 ,
GSM254548 ,
GSM254549 ,
GSM254550 ,
GSM254551 ,
GSM254552 ,
GSM254553 ,
GSM254554 ,
GSM254555 ,
GSM254556 ,
GSM254557 ,
GSM254558 ,
GSM254559 ,
GSM254560 ,
GSM254561 ,
GSM254562 ,
GSM254563 ,
GSM254564 ,
GSM254565 ,
GSM254566 ,
GSM254567 ,
GSM254568 ,
GSM254569 ,
GSM254570 ,
GSM254571 ,
GSM254572 ,
GSM254573 ,
GSM254574 ,
GSM254575 ,
GSM254576 ,
GSM254577 ,
GSM254578 ,
GSM254579 ,
GSM254580 ,
GSM254581 ,
GSM254582 ,
GSM254583 ,
GSM254584 ,
GSM254585 ,
GSM254586 ,
GSM254587 ,
GSM254588 ,
GSM254589 ,
GSM254590 ,
GSM254591 ,
GSM254592 ,
GSM254593 ,
GSM254594 ,
GSM254595 ,
GSM254596 ,
GSM254597 ,
GSM254598 ,
GSM254599 ,
GSM254600 ,
GSM254601 ,
GSM254602 ,
GSM254603 ,
GSM254604 ,
GSM254605 ,
GSM254606 ,
GSM254607 ,
GSM254608 ,
GSM254609 ,
GSM254610 ,
GSM254611 ,
GSM254612 ,
GSM254613 ,
GSM257075 ,
GSM257076 ,
GSM257077 ,
GSM257078 ,
GSM257079 ,
GSM257080 ,
GSM257081 ,
GSM257082 ,
GSM257083 ,
GSM257084 ,
GSM257085 ,
GSM257086 ,
GSM257087 ,
GSM257088 ,
GSM257089 ,
GSM257090 ,
GSM257091 ,
GSM257092 ,
GSM257093 ,
GSM257094 ,
GSM257095 ,
GSM257096 ,
GSM257097 ,
GSM257098 ,
GSM257099 ,
GSM257100 ,
GSM257101 ,
GSM257102 ,
GSM257103 ,
GSM257104 ,
GSM257105 ,
GSM257106 ,
GSM257107 ,
GSM257108 ,
GSM257109 ,
GSM257110 ,
GSM257111 ,
GSM257112 ,
GSM257113 ,
GSM257114 ,
GSM257115 ,
GSM257116 ,
GSM257117 ,
GSM257277 ,
GSM1666924 ,
GSM1666925 ,
GSM1666926 ,
GSM1666927 ,
GSM1666928 ,
GSM1666929 Series (2)
GSE6188 Distinctive microRNA profiles relating to patient survival in esophageal squamous cell carcinoma
GSE68271 Essential role of miR-200c in regulating self-renewal of breast cancer stem cells initiating from the counter parts of mammary epithelium
Data table header descriptions ID Block Block of the spot(oligonucleotide) in the chip
Column Column of the spot(oligonucleotide) in the chip
Row Row of the spot(oligonucleotide) in the chip
Name microRNA name and control name
ORF SPOT_ID
Data table ID Block Column Row Name ORF SPOT_ID 1
1
1
1
Zip5
Zip5
2
1
2
1
Zip5
Zip5
3
1
3
1
Zip5
Zip5
4
1
4
1
Zip13
Zip13
5
1
5
1
Zip13
Zip13
6
1
6
1
Zip13
Zip13
7
1
7
1
Zip15
Zip15
8
1
8
1
Zip15
Zip15
9
1
9
1
Zip15
Zip15
10
1
10
1
Zip21
Zip21
11
1
11
1
Zip21
Zip21
12
1
12
1
Zip21
Zip21
13
1
13
1
Zip23
Zip23
14
1
14
1
Zip23
Zip23
15
1
15
1
Zip23
Zip23
16
1
16
1
Zip25
Zip25
17
1
17
1
Zip25
Zip25
18
1
18
1
Zip25
Zip25
19
1
19
1
Y2
Y2
20
1
20
1
Y2
Y2
1932 70 Kbytes .Supplementary data files not provided
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