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    SUP35 translation termination factor GTPase eRF3 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851752, updated on 5-May-2024

    Summary

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    Gene symbol
    SUP35
    Gene description
    translation termination factor GTPase eRF3
    Primary source
    SGD:S000002579
    Locus tag
    YDR172W
    See related
    AllianceGenome:SGD:S000002579
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    GST1; PNM2; SAL3; SUF12; SUP2; SUP36
    Summary
    Enables ribosome binding activity and translation release factor activity. Involved in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay and translational termination. Located in cytoplasmic stress granule. Part of translation release factor complex. Orthologous to several human genes including GSPT1 (G1 to S phase transition 1). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (808324..810381)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene gag protein Neighboring gene heat shock protein HSP42 Neighboring gene inositol polyphosphate multikinase Neighboring gene Hmo1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Heterologous overexpression of Sup35 in Escherichia coli leads to both monomer and complex states. Wang M, et al. Proteins, 2022 Jul. PMID 35194850
    2. Membrane composition and lipid to protein ratio modulate amyloid kinetics of yeast prion protein. Bandyopadhyay A, et al. RSC Chem Biol, 2021 Apr 1. PMID 34458802, Free PMC Article
    3. Dangerous Stops: Nonsense Mutations Can Dramatically Increase Frequency of Prion Conversion. Dergalev AA, et al. Int J Mol Sci, 2021 Feb 3. PMID 33546497, Free PMC Article
    4. Clustering and Fibril Formation during GNNQQNY Aggregation: A Molecular Dynamics Study. Szała-Mendyk B, et al. Biomolecules, 2020 Sep 24. PMID 32987720, Free PMC Article
    5. Prion Amyloid Polymorphs - The Tag Might Change It All. Bousset L, et al. Front Mol Biosci, 2020. PMID 32850974, Free PMC Article

    See all (526) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The yeast prion protein Sup35 initiates alpha-synuclein pathology in mouse models of Parkinson's disease.
      Title: The yeast prion protein Sup35 initiates α-synuclein pathology in mouse models of Parkinson's disease.
    2. Substoichiometric Hsp104 regulates the genesis and persistence of self-replicable amyloid seeds of Sup35 prion domain.
      Title: Substoichiometric Hsp104 regulates the genesis and persistence of self-replicable amyloid seeds of Sup35 prion domain.
    3. Yeast J-protein Sis1 prevents prion toxicity by moderating depletion of prion protein.
      Title: Yeast J-protein Sis1 prevents prion toxicity by moderating depletion of prion protein.
    4. Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.
      Title: Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.
    5. Mechanisms that ensure speed and fidelity in eukaryotic translation termination.
      Title: Mechanisms that ensure speed and fidelity in eukaryotic translation termination.
    6. Clustering and Fibril Formation during GNNQQNY Aggregation: A Molecular Dynamics Study.
      Title: Clustering and Fibril Formation during GNNQQNY Aggregation: A Molecular Dynamics Study.
    7. Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Membrane Association.
      Title: Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Membrane Association.
    8. Nucleation seed size determines amyloid clearance and establishes a barrier to prion appearance in yeast.
      Title: Nucleation seed size determines amyloid clearance and establishes a barrier to prion appearance in yeast.
    9. Short disordered protein segment regulates cross-species transmission of a yeast prion.
      Title: Short disordered protein segment regulates cross-species transmission of a yeast prion.
    10. Using high-speed atomic force microscopy (HS-AFM), we directly visualized the prion determinant domain (Sup35NM) and the formation of its oligomers and fibrils at subsecond and submolecular resolutions.
      Title: Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy.
    Submit: New GeneRIF Correction See all GeneRIFs (140)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mRNA binding HDA PubMed 
    enables ribosome binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation release factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables translation release factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation release factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational termination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translational termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasmic stress granule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    part_of translation release factor complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of translation release factor complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    translation termination factor GTPase eRF3
    NP_010457.3
    • Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      808324..810381
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180479.3 → NP_010457.3  TPA: translation termination factor GTPase eRF3 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010457.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSF4, P05420, P05453
      UniProtKB/TrEMBL
      A0A023T3A2, E9P8G7, H9BH29
      Conserved Domains (2) summary
      COG5256
      Location:254 → 685
      TEF1; Translation elongation factor EF-1alpha (GTPase) [Translation, ribosomal structure and biogenesis]
      PRK10263
      Location:30 → 199
      PRK10263; DNA translocase FtsK; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P05453.1

    Gene LinkOut

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