MUS81 Mus81p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MUS81
Gene description: Mus81p
Primary source: SGD:S000002794
Locus tag: YDR386W
See related: AllianceGenome:SGD:S000002794
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SLX3
Summary: Enables enzyme inhibitor activity. Contributes to crossover junction endodeoxyribonuclease activity. Involved in DNA metabolic process and negative regulation of helicase activity. Located in nucleus. Part of Holliday junction resolvase complex. Orthologous to human MUS81 (MUS81 structure-specific endonuclease subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1246084..1247982)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.
Title: MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.
The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
Title: The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
Title: Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
Study presents evidence that yeast Mus81-Mms4 endonuclease contributes to cell viability in the absence of functional telomerase. This involvement is not through recombination-mediated telomere lengthening as proposed in humans, but rather through an indirect role involving replication stress. These and other results suggest that Mus81 localization to the telomere may be a promising indicator for telomere dysfunction.
Title: Saccharomyces cerevisiae Mus81-Mms4 prevents accelerated senescence in telomerase-deficient cells.
These results strongly support the notion that Rad52 and Mus81-Mms4 collaborate and work jointly in processing of homologous recombination intermediates.
Title: Saccharomyces cerevisiae Mus81-Mms4 and Rad52 can cooperate in the resolution of recombination intermediates.
Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
Title: Role of PCNA and RFC in promoting Mus81-complex activity.
The data suggest that subnuclear relocalization is relevant for the function of Mus81-Mms4 complex in response to DNA damage and, probably, of the endonucleases that colocalize with it.
Title: Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage.
The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation.
Title: Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis.
The data point to the possibility that the involvement of Esc2 in the metabolism of sister chromatid junctions is, in part, via modulation of the activity of the Mus81 complex.
Title: Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains.
Our data suggest that Srs2 and Mus81-Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability.
Title: Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to crossover junction DNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables crossover junction DNA endonuclease activity	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables enzyme inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA catabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA topological change	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in resolution of meiotic recombination intermediates	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Holliday junction resolvase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Holliday junction resolvase complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: Mus81p

NP_010674.3

Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA and DNA-protein cross-links; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates function of Mms4p-Mus81p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1246084..1247982

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180694.3 → NP_010674.3 TPA: Mus81p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010674.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT20, Q04149

UniProtKB/TrEMBL

A0A8H8UNT3, A6ZYY7, B3LFR4, C7GSD6, C8Z5V0, N1P8S9

Conserved Domains (2) summary

COG1948
Location:314 → 601

MUS81; ERCC4-type nuclease [Replication, recombination and repair]

cd21036
Location:132 → 224

WH_MUS81; winged helix domain found in crossover junction endonuclease MUS81 and similar proteins