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Rbx1 ring-box 1 [ Mus musculus (house mouse) ]

Gene ID: 56438, updated on 19-Nov-2025

Summary

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Official Symbol: Rbx1provided by MGI
Official Full Name: ring-box 1provided by MGI
Primary source: MGI:MGI:1891829
See related: Ensembl:ENSMUSG00000022400 AllianceGenome:MGI:1891829
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ROC1; 1500002P15Rik
Summary: Predicted to enable several functions, including cullin family protein binding activity; ubiquitin protein ligase binding activity; and ubiquitin-like protein ligase activity. Predicted to contribute to ubiquitin-protein transferase activity. Involved in several processes, including SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; cellular response to UV; and protein ubiquitination. Acts upstream of or within protein K48-linked ubiquitination; protein catabolic process; and protein monoubiquitination. Located in cytosol. Part of Cul4A-RING E3 ubiquitin ligase complex and SCF ubiquitin ligase complex. Is expressed in several structures, including genitourinary system. Orthologous to human RBX1 (ring-box 1). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 43.0), CNS E14 (RPKM 40.8) and 26 other tissues See more
Orthologs: human all
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Genomic context

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See Rbx1 in Genome Data Viewer

Location:: 15 E1; 15 38.11 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (81350517..81360570)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (81466316..81476369)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RING box protein-1(RBX1), a key component of SCF E3 ligase, induced multiple myeloma cell drug-resistance though suppressing p27.
Title: RING box protein-1(RBX1), a key component of SCF E3 ligase, induced multiple myeloma cell drug-resistance though suppressing p27.
The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness.
Title: The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness.
Downregulation of Rbx1 caused accumulation of Emi1.
Title: The role of RING box protein 1 in mouse oocyte meiotic maturation.
a model by which N-terminal cleavage of RBX1 impairs its activity and promotes susceptibility to ER stress induction.
Title: Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells.
Nrf2 regulates Cul3-Rbx1 by controlling regulation of expression and induction of Cul3-Rbx1
Title: An autoregulatory loop between Nrf2 and Cul3-Rbx1 controls their cellular abundance.
in vivo physiological function of RBX1 is to ensure cell proliferation by preventing p27 accumulation during the early stage of embryonic development
Title: RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27.
SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation
Title: SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Gene trapped (2) 1 citation
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237362

RH124284 (e-PCR)
- UniSTS:161106

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NEDD8 ligase activity	IEA Inferred from Electronic Annotation more info
enables NEDD8 ligase activity	ISO Inferred from Sequence Orthology more info
enables NEDD8 ligase activity	ISS Inferred from Sequence or Structural Similarity more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables cullin family protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables cullin family protein binding	IEA Inferred from Electronic Annotation more info
enables cullin family protein binding	ISO Inferred from Sequence Orthology more info
enables eukaryotic initiation factor 4E binding	TAS Traceable Author Statement more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
contributes_to ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
contributes_to ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
contributes_to ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin-protein transferase activity	TAS Traceable Author Statement more info	PubMed
enables ubiquitin-ubiquitin ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-ubiquitin ligase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	EXP Inferred from Experiment more info	PubMed
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA damage response, signal transduction by p53 class mediator	ISO Inferred from Sequence Orthology more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within G1/S transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in RNA polymerase II transcription initiation surveillance	IEA Inferred from Electronic Annotation more info
involved_in RNA polymerase II transcription initiation surveillance	ISO Inferred from Sequence Orthology more info
involved_in RNA polymerase II transcription initiation surveillance	ISS Inferred from Sequence or Structural Similarity more info
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in T cell activation	IEA Inferred from Electronic Annotation more info
involved_in T cell activation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within TORC1 signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within autophagosome assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within base-excision repair, AP site formation via deaminated base removal	ISO Inferred from Sequence Orthology more info
involved_in cellular response to UV	EXP Inferred from Experiment more info	PubMed
involved_in cellular response to UV	IEA Inferred from Electronic Annotation more info
involved_in cellular response to UV	ISO Inferred from Sequence Orthology more info
involved_in cellular response to UV	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cellular response to amino acid starvation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to amino acid stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to amino acid stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to nutrient levels	ISO Inferred from Sequence Orthology more info
involved_in cellular response to oxidative stress	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in cellular response to oxidative stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within epithelial to mesenchymal transition	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lysosome organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within miRNA-mediated gene silencing by mRNA destabilization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mitophagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of insulin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of response to oxidative stress	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of response to oxidative stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of signal transduction by p53 class mediator	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of type I interferon production	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of type I interferon production	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within p38MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of TORC1 signaling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of epithelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein autoubiquitination	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein autoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of translation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of type I interferon production	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein K27-linked ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein K27-linked ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein monoubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein monoubiquitination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein monoubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein neddylation	IEA Inferred from Electronic Annotation more info
involved_in protein neddylation	ISO Inferred from Sequence Orthology more info
involved_in protein neddylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in signal transduction in response to DNA damage	IEA Inferred from Electronic Annotation more info
involved_in signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in spermatogenesis	IEA Inferred from Electronic Annotation more info
involved_in spermatogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within transcription elongation by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in transcription-coupled nucleotide-excision repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within transcription-coupled nucleotide-excision repair	ISO Inferred from Sequence Orthology more info
involved_in transcription-coupled nucleotide-excision repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within type I interferon-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of Cul2-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul2-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul3-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul3-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul3-RING ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of Cul4-RING E3 ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul4-RING E3 ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul4A-RING E3 ubiquitin ligase complex	EXP Inferred from Experiment more info	PubMed
part_of Cul4A-RING E3 ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul4A-RING E3 ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul4A-RING E3 ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of Cul4B-RING E3 ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul4B-RING E3 ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul4B-RING E3 ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of Cul5-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul5-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul7-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul7-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul7-RING ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SCF ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of SCF ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of SCF ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of SCF ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
part_of VCB complex	ISO Inferred from Sequence Orthology more info
part_of anaphase-promoting complex	TAS Traceable Author Statement more info	PubMed
part_of cullin-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cullin-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of cullin-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
part_of nucleotide-excision repair complex	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
is_active_in site of DNA damage	IEA Inferred from Electronic Annotation more info
is_active_in site of DNA damage	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RBX1

Names: E3 ubiquitin-protein transferase RBX1; RING finger protein 75; ring-box protein 1

NP_062686.1

EC 2.3.2.27

EC 2.3.2.32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019712.3 → NP_062686.1 E3 ubiquitin-protein ligase RBX1

See identical proteins and their annotated locations for NP_062686.1

Status: VALIDATED

Source sequence(s)

BB807275, BC056992, CB522825, CJ082632

Consensus CDS

CCDS49676.1

UniProtKB/Swiss-Prot

P62878

Related

ENSMUSP00000023036.6, ENSMUST00000023036.7

Conserved Domains (1) summary

cd16485
Location:40 → 101

mRING-H2-C3H2C2D_RBX1; modified RING finger, H2 subclass (C3H2C2D-type), found in RING-box protein 1 (RBX1) and similar proteins