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nanS N-acetyl-9-O-acetylneuraminate esterase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948835, updated on 3-Jun-2026
Official Symbol
nanS
Official Full Name
N-acetyl-9-O-acetylneuraminate esterase
Primary source
ECOCYC:G7919
Locus tag
b4309
See related
ASAP:ABE-0014130
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4300; yjhS
Summary
yjhS is essential for growth on glycerol minimal medium . [More information is available at EcoCyc: G7919].
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Sequence:
NC_000913.3 (4536614..4537594, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene pseudo Neighboring gene protein YthB Neighboring gene N-acetylneuraminate mutarotase Neighboring gene N-acetylneuraminate outer membrane channel

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. De-O-Acetylation of mucin-derived sialic acids by recombinant NanS-p esterases of Escherichia coli O157:H7 strain EDL933. Feuerbaum S, et al. Int J Med Microbiol, 2018 Dec. PMID 30340996, Free PMC Article
  2. Unexpected Diversity of Escherichia coli Sialate O-Acetyl Esterase NanS. Rangel A, et al. J Bacteriol, 2016 Oct 15. PMID 27481927, Free PMC Article
  3. Structural and enzymatic characterization of NanS (YjhS), a 9-O-Acetyl N-acetylneuraminic acid esterase from Escherichia coli O157:H7. Rangarajan ES, et al. Protein Sci, 2011 Jul. PMID 21557376, Free PMC Article
  4. YjhS (NanS) is required for Escherichia coli to grow on 9-O-acetylated N-acetylneuraminic acid. Steenbergen SM, et al. J Bacteriol, 2009 Nov. PMID 19749043, Free PMC Article
  5. Sialic acid mutarotation is catalyzed by the Escherichia coli beta-propeller protein YjhT. Severi E, et al. J Biol Chem, 2008 Feb 22. PMID 18063573

See all (11) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. An expression of sialic acid O-acetylesterases, NanS and NanS-p with a broad specificity could be an advantage in competition with the gut microbiota for nutrients and facilitate EHEC colonization in the human large intestine.
    Title: De-O-Acetylation of mucin-derived sialic acids by recombinant NanS-p esterases of Escherichia coli O157:H7 strain EDL933.
  2. The catabolism of the diacetylated form of host sialic acid requires a specialized esterase, NanS.
    Title: Unexpected Diversity of Escherichia coli Sialate O-Acetyl Esterase NanS.
  3. Data show that YjhS (NanS) is a probable 9-O-acetyl N-acetylneuraminic acid esterase required for E. coli to grow on this alternative sialic acid, which is commonly found in mammalian host mucosal sites.
    Title: YjhS (NanS) is required for Escherichia coli to grow on 9-O-acetylated N-acetylneuraminic acid.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carboxylic ester hydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hydrolase activity, acting on ester bonds IEA
Inferred from Electronic Annotation
more info
  
enables sialate O-acetylesterase activity IC
Inferred by Curator
more info
 PubMed 
enables sialate O-acetylesterase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sialate O-acetylesterase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within N-acetylneuraminate metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in carboxylic acid metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
N-acetyl-9-O-acetylneuraminate esterase
NP_418729.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4536614..4537594 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418729.1 N-acetyl-9-O-acetylneuraminate esterase

    See identical proteins and their annotated locations for NP_418729.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A5C9AP61
    Conserved Domains (1) summary
    pfam03629
    Location:11 → 209
    SASA; Carbohydrate esterase, sialic acid-specific acetylesterase
Nucleotide Protein
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