pepA aminopeptidase A/I [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Gene symbol: pepA
Gene description: aminopeptidase A/I
Primary source: ASAP:ABE-0013953
Locus tag: b4260
See related: ECOCYC:EG10694
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: carP; ECK4253; xerB
Summary: PepA has a broad specificity for successive removal of many N-terminal amino acids, although leucine is preferred as the residue N-terminal to the cleavage site. [More information is available at EcoGene: EG10694]. Aminopeptidase A/I is a peptidase that binds DNA and is required for maintenance of plasmid monomers, which is critical for proper plasmid segregation . [More information is available at EcoCyc: EG10694].
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NC_000913.3 Genomic Context describing neighboring genes

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in vitro topology studies performed with DNA minicircles demonstrate that PepA binding constrains a single positive supercoil in the carP1 control region. Such a topological event is understood to constitute an impediment to transcription initiation and may serve as a mechanism to regulate gene expression.
Title: The trigger enzyme PepA (aminopeptidase A) of Escherichia coli, a transcriptional repressor that generates positive supercoiling.
Data show that PepA can effectively process heptapeptide-nucleotide microcin C (McC).
Title: Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
N-terminus verified by Edman degradation on complete protein
Title: xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.
Random PepA mutants unable to support efficient Xer recombination are reported.
Title: Mutagenesis of PepA suggests a new model for the Xer/cer synaptic complex.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables aminopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables aminopeptidase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables manganese ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloexopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables transcription antitermination factor activity, DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within peptide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within plasmid maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within plasmid recombination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within transcription antitermination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within transcription, DNA-templated	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within transcription, DNA-templated	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

NP_418681.1

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