marA DNA-binding transcriptional dual regulator MarA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: marA
Official Full Name: DNA-binding transcriptional dual regulator MarA
Primary source: ECOCYC:EG11434
Locus tag: b1531
See related: ASAP:ABE-0005112
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: cfxB; ECK1524; inaR; nfxC; norB; soxQ
Summary: Martin (2002) provide some evidence in support of the this alternative ATG start: native protein is slightly smaller than the engineered protein by Western blot. [More information is available at EcoGene: EG11434]. MarA (multiple antibiotic resistance) belongs to the AraC/XylS family. [More information is available at EcoCyc: EG11434].
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Genomic context

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Sequence:: NC_000913.3 (1619574..1619957)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition.
Title: The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition.
MarA is a regulator of biofilm formation. Control is mediated by binding of MarA to the intergenic region upstream of the ycgZ-ymgABC operon.
Title: The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation.
MarA controls a variety of stress response genes. We modify its half-life either by knocking down the protease that targets it via CRISPRi or by engineering MarA to protect it from degradation. Our experimental and analytical results indicate that active degradation can impact both the rate of coordination and the maximum coordination that downstream genes can achieve.
Title: Active degradation of MarA controls coordination of its downstream targets.
Transcription factors MarR and MarA confer multidrug resistance in enteric bacteria by modulating efflux pump and porin expression. Here, the authors show that MarA also upregulates genes required for lipid trafficking and DNA repair, thus reducing antibiotic entry and quinolone-induced DNA damage.
Title: The multiple antibiotic resistance operon of enteric bacteria controls DNA repair and outer membrane integrity.
Genetic defects in SoxS, MarA, or AcrB underlie superoxide-mediated protection of Escherichia coli from antimicrobials.
Title: Superoxide-mediated protection of Escherichia coli from antimicrobials.
MarB encodes a periplasmic protein which reduces the transcription rate of marA.
Title: The 216-bp marB gene of the marRAB operon in Escherichia coli encodes a periplasmic protein which reduces the transcription rate of marA.
Replacement of the MarA glutamic acid residue 89 with alanine greatly increased the marbox binding and activation of many class I promoters.
Title: Promoter discrimination at class I MarA regulon promoters mediated by glutamic acid 89 of the MarA transcriptional activator of Escherichia coli.
roles of the Escherichia coli transcriptional regulators MarA, SoxS and Rob in susceptibility to cationic antimicrobial peptides
Title: Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon.
Data that TolC is involved in the efflux of certain cellular metabolites that accumulate during growth and trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites.
Title: An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants.
The authors show here that tolC has two additional strong overlapping promoters, p3 and p4, which are downstream of p1, p2 and the marbox and are activated by MarA, SoxS and Rob.
Title: Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli.

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding transcriptional dual regulator MarA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1619574..1619957

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416048.2 DNA-binding transcriptional dual regulator MarA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416048.2

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A1X3I7G7, A0A1X3J229, A0A1X3JHI0, A0A829LEV6, D8AE57, Q7BF81

Conserved Domains (1) summary

PRK11511
Location:1 → 127

PRK11511; MDR efflux pump AcrAB transcriptional activator MarA