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hypC hydrogenase maturation factor HypC [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947192, updated on 10-Oct-2024

Summary

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Official Symbol
hypC
Official Full Name
hydrogenase maturation factor HypC
Primary source
ECOCYC:EG10485
Locus tag
b2728
See related
ASAP:ABE-0008962
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2723
Summary
N-terminal Cys is required for function. hypC mutants have reduced Hyd-2 activity. [More information is available at EcoGene: EG10485]. The HypC protein is an accessory protein primarily involved in the maturation of the hydrogenase 3 isoenzyme. [More information is available at EcoCyc: EG10485].
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Genomic context

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Sequence:
NC_000913.3 (2851864..2852136)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene regulator of the transcriptional regulator FhlA Neighboring gene hydrogenase 3 nickel incorporation protein HypA Neighboring gene hydrogenase isoenzymes nickel incorporation protein HypB Neighboring gene Fe-(CN)2CO cofactor assembly scaffold protein HypD Neighboring gene carbamoyl dehydratase HypE Neighboring gene DNA-binding transcriptional activator FhlA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Solution structure of Escherichia coli HypC. Wang L, et al. Biochem Biophys Res Commun, 2007 Sep 28. PMID 17669368
  2. Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide insight into [NiFe]-hydrogenase large subunit recognition by the HypC protein family. Hartwig S, et al. Microbiology (Reading), 2015 Nov. PMID 26364315
  3. Dissection of the maturation reactions of the [NiFe] hydrogenase 3 from Escherichia coli taking place after nickel incorporation. Magalon A, et al. FEBS Lett, 2000 May 12. PMID 10812085
  4. Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle. Blokesch M, et al. J Mol Biol, 2002 Nov 22. PMID 12441107
  5. The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli. Jacobi A, et al. Arch Microbiol, 1992. PMID 1482271

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Together, these findings identify amino acid regions in HypC accessory proteins that specify interaction with the large subunits of hydrogenase and demonstrate functional compatibility of Hyp accessory protein machineries.
    Title: Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide insight into [NiFe]-hydrogenase large subunit recognition by the HypC protein family.
  2. Sequence alignment and structural comparison reveal that the hydrophobic region on the surface of E. coli HypC, as well as the highly flexible C-terminal helixes, may involve in the interactions of E. coli HypC with other proteins.
    Title: Solution structure of Escherichia coli HypC.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Analysis of the HypC-hycE complex, a key intermediate in the assembly of the metal center of the Escherichia coli hydrogenase 3.
  4. dynamic complex between the hydrogenase maturation proteins HypD, HypC, HypE and HypF, which is the site of ligand biosynthesis and attachment to the iron atom of the NiFe site in hydrogenase
    Title: The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carbon dioxide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables carbon dioxide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables iron ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein maturation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein maturation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein maturation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein-containing complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
hydrogenase maturation factor HypC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2851864..2852136
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417208.1 hydrogenase maturation factor HypC [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417208.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1X3I1J2, A0A1X3JDQ1, A0A8S0FMP1, A0AAJ3NX78, A0AAP7VTK2, F4T456
    Conserved Domains (1) summary
    PRK10409
    Location:1 → 90
    PRK10409; HypC/HybG/HupF family hydrogenase formation chaperone
Nucleotide Protein
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