U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

glyA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947022, updated on 24-Aug-2024

Summary

Go to the top of the page Help
Official Symbol
glyA
Primary source
ASAP:ABE-0008389
Locus tag
b2551
See related
ECOCYC:EG10408
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2548
Summary
Serine hydroxymethyltransferase converts serine to glycine, transferring a methyl group to tetrahydrofolate, thus forming 5,10-methylene-tetrahydrofolate (5,10-mTHF). [More information is available at EcoCyc: EG10408].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (2684254..2685507, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF5107 domain-containing protein YphG Neighboring gene putative DNA-binding transcriptional regulator, NAGC-like Neighboring gene nitric oxide dioxygenase Neighboring gene nitrogen regulatory protein PII-1

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase--the role of evolutionarily conserved hydrophobic contacts. Florio R, et al. FEBS J, 2009 Dec. PMID 19909338
  2. The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase. Florio R, et al. FEBS J, 2009 Jan. PMID 19019081
  3. The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase. Malerba F, et al. Biochem J, 2007 Jun 15. PMID 17341210, Free PMC Article
  4. Serine hydroxymethyltransferase revisited. Schirch V, et al. Curr Opin Chem Biol, 2005 Oct. PMID 16125438
  5. GLYCINE SYNTHESIS AND METABOLISM IN ESCHERICHIA COLI. PIZER LI. J Bacteriol, 1965 Apr. PMID 14276110, Free PMC Article

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli.
    Title: Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli.
  2. A bioinformatic study shows that Tyr55 (at the active site) and Arg235 are strongly conserved within the serine hydroxymethyltransferase family and are involved in a cation-pi interaction in the large majority of available SHMT structures.
    Title: Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase.
  3. N-terminus verified by Edman degradation on complete protein
    Title: The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli.
  4. Results describe the mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase.
    Title: The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase.
  5. activation of glyA by CsgD requires the participation of the regulatory proteins, MetR and PurR
    Title: Role of MetR and PurR in the activation of glyA by CsgD in Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-allo-threonine aldolase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables glycine hydroxymethyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycine hydroxymethyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycine hydroxymethyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycine hydroxymethyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within L-serine biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within L-serine catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycine biosynthetic process from serine IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within glycine biosynthetic process from serine IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycine biosynthetic process from serine IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycine biosynthetic process from serine IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within glycine catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

Go to the top of the page Help
Preferred Names
serine hydroxymethyltransferase
NP_417046.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2684254..2685507 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417046.1 serine hydroxymethyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417046.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7T8K8
    Conserved Domains (1) summary
    PRK00011
    Location:6 → 417
    glyA; serine hydroxymethyltransferase; Reviewed
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.