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truA tRNA pseudouridine(38-40) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946793, updated on 19-Sep-2024

Summary

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Official Symbol
truA
Official Full Name
tRNA pseudouridine(38-40) synthase
Primary source
ECOCYC:EG10454
Locus tag
b2318
See related
ASAP:ABE-0007657
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
asuC; ECK2312; hisT; leuK
Summary
[More information is available at EcoGene: EG10454]. TruA is the tRNA pseudouridine synthase responsible for catalyzing pseudouridine formation in the anticodon loop of a subset of tRNAs . [More information is available at EcoCyc: EG10454].
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Genomic context

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Sequence:
NC_000913.3 (2434824..2435636, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene acetyl-CoA carboxyltransferase subunit beta Neighboring gene DedA family protein DedA Neighboring gene putative semialdehyde dehydrogenase Usg Neighboring gene erythronate-4-phosphate dehydrogenase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The mechanism of pseudouridine synthase I as deduced from its interaction with 5-fluorouracil-tRNA. Gu X, et al. Proc Natl Acad Sci U S A, 1999 Dec 7. PMID 10588695, Free PMC Article
  2. A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst. Huang L, et al. Biochemistry, 1998 Jan 6. PMID 9425056
  3. The role of cysteine residues in the rearrangement of uridine to pseudouridine catalyzed by pseudouridine synthase I. Zhao X, et al. J Biol Chem, 1997 Jan 17. PMID 8999885
  4. Specific mistranslation in hisT mutants of Escherichia coli. Parker J. Mol Gen Genet, 1982. PMID 6757659
  5. Escherichia coli B/r leuK mutant lacking pseudouridine synthase I activity. Searles LL, et al. J Bacteriol, 1986 Apr. PMID 3514581, Free PMC Article

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TruA displayed a rate constant of pseudouridine formation of 0.35 sec(-1).
    Title: Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I.
  3. molecular basis for the site and substrate "promiscuity" was studied by determining the crystal structures of TruA in complex with two different leucyl tRNAs
    Title: How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity, acting on RNA IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pseudouridine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA pseudouridine(38-40) synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA pseudouridine synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
tRNA pseudouridine(38-40) synthase
NP_416821.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2434824..2435636 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416821.1 tRNA pseudouridine(38-40) synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416821.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6D0IGG0
    Conserved Domains (1) summary
    PRK00021
    Location:11 → 252
    truA; tRNA pseudouridine(38-40) synthase TruA
Nucleotide Protein
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