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trpA tryptophan synthase subunit alpha [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946204, updated on 19-Sep-2024

Summary

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Official Symbol
trpA
Official Full Name
tryptophan synthase subunit alpha
Primary source
ECOCYC:EG11024
Locus tag
b1260
See related
ASAP:ABE-0004232
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1254; try; tryp
Summary
otherPDB (S. typhimurium): [More information is available at EcoGene: EG11024]. The TrpA polypeptide (TSase ) functions as the subunit of the tetrameric (2-2) tryptophan synthase complex. [More information is available at EcoCyc: EG11024].
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Genomic context

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Sequence:
NC_000913.3 (1316416..1317222, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF892 domain-containing protein YciF Neighboring gene stress-induced bacterial acidophilic repeat motifs-containing protein YciG Neighboring gene tryptophan synthase subunit beta Neighboring gene fused indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase Neighboring gene anthranilate synthase subunit TrpD Neighboring gene anthranilate synthase subunit TrpE

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Long-range interactions in the α subunit of tryptophan synthase help to coordinate ligand binding, catalysis, and substrate channeling. Axe JM, et al. J Mol Biol, 2013 May 13. PMID 23376097
  2. NMR analysis of partially folded states and persistent structure in the alpha subunit of tryptophan synthase: implications for the equilibrium folding mechanism of a 29-kDa TIM barrel protein. Vadrevu R, et al. J Mol Biol, 2008 Mar 14. PMID 18234216, Free PMC Article
  3. A tightly packed hydrophobic cluster directs the formation of an off-pathway sub-millisecond folding intermediate in the alpha subunit of tryptophan synthase, a TIM barrel protein. Wu Y, et al. J Mol Biol, 2007 Mar 9. PMID 17222865, Free PMC Article
  4. A molecular characterization of spontaneous frameshift mutagenesis within the trpA gene of Escherichia coli. Hardin A, et al. DNA Repair (Amst), 2007 Feb 4. PMID 17084112, Free PMC Article
  5. Specific structure appears at the N terminus in the sub-millisecond folding intermediate of the alpha subunit of tryptophan synthase, a TIM barrel protein. Wu Y, et al. J Mol Biol, 2005 Aug 19. PMID 16023136

See all (93) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Allosteric networks intrinsic to alpha tryptophan synthase (alphaTS) are modulated by binding of the beta-subunit to regulate alphaTS function, thus giving insight into coordination of the two active sites.
    Title: Long-range interactions in the α subunit of tryptophan synthase help to coordinate ligand binding, catalysis, and substrate channeling.
  2. The conversion of the more stable intermediate to the native state of alpha TS entails the formation of a stable helical shell and completes the acquisition of the tertiary structure.
    Title: NMR analysis of partially folded states and persistent structure in the alpha subunit of tryptophan synthase: implications for the equilibrium folding mechanism of a 29-kDa TIM barrel protein.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  4. protection observed in a contiguous set of beta-strands and alpha-helices in the N terminus implies a significant role for this sub-domain in directing the folding of this TIM barrel protein
    Title: An obligatory intermediate controls the folding of the alpha-subunit of tryptophan synthase, a TIM barrel protein.
  5. The molecular origins of the trpA frameshifts analyzed could be explained by one of six simple models based upon misalignment of the template or nascent DNA strands with or without misincorporation of primer nucleotides during DNA replication.
    Title: A molecular characterization of spontaneous frameshift mutagenesis within the trpA gene of Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables molecular adaptor activity EXP
Inferred from Experiment
more info
 PubMed 
contributes_to tryptophan synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tryptophan synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within aromatic amino acid family biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tryptophan biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tryptophan biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tryptophan biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tryptophan biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tryptophan metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm NAS
Non-traceable Author Statement
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tryptophan synthase subunit alpha
NP_415776.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1316416..1317222 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415776.1 tryptophan synthase subunit alpha [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415776.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2X1NI16, A0A7L7E6Z5, A0A8S7T3L1
    Conserved Domains (1) summary
    TIGR00262
    Location:8 → 263
    trpA; tryptophan synthase, alpha subunit
Nucleotide Protein
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