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pykF pyruvate kinase 1 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946179, updated on 10-Oct-2024

Summary

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Official Symbol
pykF
Official Full Name
pyruvate kinase 1
Primary source
ECOCYC:EG10804
Locus tag
b1676
See related
ASAP:ABE-0005600
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1672
Summary
Pyruvate kinase I and pyruvate kinase II differ in physical and chemical properties as well as in their kinetic behavior. [More information is available at EcoCyc: EG10804].
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Genomic context

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Sequence:
NC_000913.3 (1755698..1757110)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene fumarase D Neighboring gene protein YnhH Neighboring gene murein lipoprotein Neighboring gene L,D-transpeptidase LdtE

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Direct genomic sequencing of bacterial DNA: the pyruvate kinase I gene of Escherichia coli. Ohara O, et al. Proc Natl Acad Sci U S A, 1989 Sep. PMID 2674937, Free PMC Article
  2. Crystal structure of Escherichia coli pyruvate kinase type I: molecular basis of the allosteric transition. Mattevi A, et al. Structure, 1995 Jul 15. PMID 8591049
  3. Analysis of progress curves. Rate law of pyruvate kinase type I from Escherichia coli. Markus M, et al. Biochem J, 1980 Sep 1. PMID 7011316, Free PMC Article
  4. AMP- and fructose 1,6-bisphosphate-activated pyruvate kinases from Escherichia coli. Malcovati M, et al. Methods Enzymol, 1982. PMID 6759852
  5. The control of pyruvate kinases of Escherichia coli. I. Physicochemical and regulatory properties of the enzyme activated by fructose 1,6-diphosphate. Waygood EB, et al. J Biol Chem, 1974 Jan 10. PMID 4588693

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. data are consistent with the domain rotation model but inconsistent with the rigid body reorientation model given the increased flexibility at the interdomain interface, and we can for the first time explain how fructose 1,6-bisphosphate affects the active site.
    Title: Conformational Dynamics and Allostery in Pyruvate Kinase.
  2. The elevated cAMP in PB25 suggests that pyruvate kinase may participate in glucose catabolite repression by serving among all of the factors that tighten gene expression.
    Title: Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  4. A pykF-gene knockout mutation effects gene expression and metabolic fluxes in Escherichia coli.
    Title: Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables potassium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables pyruvate kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pyruvate kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyruvate kinase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within glycolytic process EXP
Inferred from Experiment
more info
 PubMed 
involved_in glycolytic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glycolytic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphorylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to heat IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 
part_of pyruvate kinase complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
pyruvate kinase 1
NP_416191.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1755698..1757110
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416191.1 pyruvate kinase 1 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416191.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376WY06
    Conserved Domains (1) summary
    PRK09206
    Location:1 → 470
    PRK09206; pyruvate kinase PykF
Nucleotide Protein
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