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kdsA 3-deoxy-D-manno-octulosonate 8-phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945785, updated on 6-Jun-2024

Summary

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Gene symbol
kdsA
Gene description
3-deoxy-D-manno-octulosonate 8-phosphate synthase
Primary source
ASAP:ABE-0004077
Locus tag
b1215
See related
ECOCYC:EG10518
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1203
Summary
kdsA gutQ double mutants are non-viable and lack KDO; multicopy msbA, or unknown chromsomal mutation(s) in suppressed KDO-null strain KPM22, permit viabilty without KDO-containing LPS (Meredith, 2006). [More information is available at EcoGene: EG10518]. 3-deoxy-D-manno-octulosonate 8-phosphate (KDO-8-P) synthase is a key enzyme in lipopolysaccharide biosynthesis. [More information is available at EcoCyc: EG10518].
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Genomic context

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Sequence:
NC_000913.3 (1268165..1269019)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein-(glutamine-N(5)) methyltransferase Neighboring gene SirB family protein YchQ Neighboring gene transglutaminase-like/TPR repeat-containing protein Neighboring gene small toxic polypeptide LdrA Neighboring gene ncRNA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural and mechanistic changes along an engineered path from metallo to nonmetallo 3-deoxy-D-manno-octulosonate 8-phosphate synthases. Kona F, et al. Biochemistry, 2007 Apr 17. PMID 17381075
  2. Crystal structures of Escherichia coli KDO8P synthase complexes reveal the source of catalytic irreversibility. Vainer R, et al. J Mol Biol, 2005 Aug 19. PMID 16023668
  3. Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range. Li Z, et al. Anal Biochem, 2005 Aug 1. PMID 15979047
  4. A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli. Shulami S, et al. J Biol Chem, 2004 Oct 22. PMID 15308670
  5. Designing a metal-binding site in the scaffold of Escherichia coli KDO8PS. Oliynyk Z, et al. Protein Eng Des Sel, 2004 Apr. PMID 15166313

See all (42) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  2. a mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli
    Title: A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-deoxy-8-phosphooctulonate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3-deoxy-8-phosphooctulonate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3-deoxy-8-phosphooctulonate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
3-deoxy-D-manno-octulosonate 8-phosphate synthase
NP_415733.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1268165..1269019
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415733.1 3-deoxy-D-manno-octulosonate 8-phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415733.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1V2T1J3, A0A2W6PFE8, A0A775X2E9
    Conserved Domains (1) summary
    COG2877
    Location:3 → 282
    KdsA; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
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