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kdsA 3-deoxy-D-manno-octulosonate 8-phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945785, updated on 30-Jul-2025
Official Symbol
kdsA
Official Full Name
3-deoxy-D-manno-octulosonate 8-phosphate synthase
Primary source
ECOCYC:EG10518
Locus tag
b1215
See related
ASAP:ABE-0004077
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1203
Summary
kdsA gutQ double mutants are non-viable and lack KDO; multicopy msbA, or unknown chromsomal mutation(s) in suppressed KDO-null strain KPM22, permit viabilty without KDO-containing LPS (Meredith, 2006). [More information is available at EcoGene: EG10518]. 3-deoxy-D-manno-octulosonate 8-phosphate (KDO-8-P) synthase is a key enzyme in lipopolysaccharide biosynthesis. [More information is available at EcoCyc: EG10518].
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Sequence:
NC_000913.3 (1268165..1269019)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein-(glutamine-N(5)) methyltransferase Neighboring gene SirB family protein YchQ Neighboring gene transglutaminase-like/TPR repeat-containing protein Neighboring gene small toxic polypeptide LdrA Neighboring gene ncRNA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase. The use of synthetic analogues to probe the structure of the putative reaction intermediate. Baasov T, et al. Eur J Biochem, 1993 Nov 1. PMID 8223657
  2. Structure and mechanism of 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Radaev S, et al. J Biol Chem, 2000 Mar 31. PMID 10734095
  3. Preliminary X-ray analysis of a new crystal form of the Escherichia coli KDO8P synthase. Radaev S, et al. Acta Crystallogr D Biol Crystallogr, 2000 Apr. PMID 10739938
  4. 3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate. Wagner T, et al. J Mol Biol, 2000 Aug 11. PMID 10926505
  5. Crystal structures of KDOP synthase in its binary complexes with the substrate phosphoenolpyruvate and with a mechanism-based inhibitor. Asojo O, et al. Biochemistry, 2001 May 29. PMID 11371194

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  2. a mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli
    Title: A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-deoxy-8-phosphooctulonate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3-deoxy-8-phosphooctulonate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3-deoxy-8-phosphooctulonate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carboxylic acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
3-deoxy-D-manno-octulosonate 8-phosphate synthase
NP_415733.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1268165..1269019
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415733.1 3-deoxy-D-manno-octulosonate 8-phosphate synthase

    See identical proteins and their annotated locations for NP_415733.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1V2T1J3, A0A2W6PFE8, A0A775X2E9
    Conserved Domains (1) summary
    COG2877
    Location:3 → 282
    KdsA; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
Heading Accession and Version

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