Format

Send to:

Choose Destination

ASAP2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Homo sapiens (human) ]

Gene ID: 8853, updated on 9-Dec-2018

Summary

Official Symbol
ASAP2provided by HGNC
Official Full Name
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2provided by HGNC
Primary source
HGNC:HGNC:2721
See related
Ensembl:ENSG00000151693 MIM:603817
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
Summary
This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Expression
Broad expression in testis (RPKM 24.6), kidney (RPKM 11.9) and 21 other tissues See more
Orthologs

Genomic context

See ASAP2 in Genome Data Viewer
Location:
2p25.1; 2p24
Exon count:
32
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 2 NC_000002.12 (9206765..9405683)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (9346894..9545812)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105373417 Neighboring gene uncharacterized LOC100506317 Neighboring gene uncharacterized LOC107985851 Neighboring gene eukaryotic translation initiation factor 1 pseudogene 7 Neighboring gene integrin subunit beta 1 binding protein 1 Neighboring gene cleavage and polyadenylation specific factor 3 Neighboring gene isoamyl acetate hydrolyzing esterase 1 (putative) Neighboring gene ADAM metallopeptidase domain 17

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Phenotypes

NHGRI GWAS Catalog

Description
Genome-wide association analysis of susceptibility and clinical phenotype in multiple sclerosis.
NHGRI GWA Catalog

Pathways from BioSystems

  • Arf6 trafficking events, organism-specific biosystem (from Pathway Interaction Database)
    Arf6 trafficking events, organism-specific biosystem
    Arf6 trafficking events
  • Endocytosis, organism-specific biosystem (from KEGG)
    Endocytosis, organism-specific biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
  • Endocytosis, conserved biosystem (from KEGG)
    Endocytosis, conserved biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
  • Fc gamma R-mediated phagocytosis, organism-specific biosystem (from KEGG)
    Fc gamma R-mediated phagocytosis, organism-specific biosystemPhagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes ta...
  • Fc gamma R-mediated phagocytosis, conserved biosystem (from KEGG)
    Fc gamma R-mediated phagocytosis, conserved biosystemPhagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes ta...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ42910, KIAA0400

Gene Ontology Provided by GOA

Function Evidence Code Pubs
GTPase activator activity IEA
Inferred from Electronic Annotation
more info
 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
positive regulation of GTPase activity IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
Golgi cisterna membrane IEA
Inferred from Electronic Annotation
more info
 
plasma membrane IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
Names
PYK2 C terminus-associated protein
centaurin, beta 3
development and differentiation-enhancing factor 2
paxillin-associated protein with ARF GAP activity 3
pyk2 C-terminus-associated protein

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001135191.1NP_001128663.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b

    See identical proteins and their annotated locations for NP_001128663.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is lacking a 45-aa portion of the proline-rich region, compared to isoform a.
    Source sequence(s)
    AA918883, AB007860, AC079782, BC063308
    Consensus CDS
    CCDS46224.1
    UniProtKB/Swiss-Prot
    O43150
    Related
    ENSP00000316404.4, ENST00000315273.4
    Conserved Domains (8) summary
    cd07642
    Location:34248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:903958
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:579673
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:297403
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:306393
    PH; PH domain
    pfam01412
    Location:421538
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:607661
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:586618
    ANK; ANK repeat [structural motif]
  2. NM_003887.3NP_003878.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform a

    See identical proteins and their annotated locations for NP_003878.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
    Source sequence(s)
    AA918883, AB007860, AC079782
    Consensus CDS
    CCDS1661.1
    UniProtKB/Swiss-Prot
    O43150
    Related
    ENSP00000281419.3, ENST00000281419.7
    Conserved Domains (8) summary
    cd07642
    Location:34248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:9481003
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:579673
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:297403
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:306393
    PH; PH domain
    pfam01412
    Location:421538
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:607661
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:586618
    ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p12 Primary Assembly

    Range
    9206765..9405683
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006711898.1XP_006711961.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3

    Conserved Domains (8) summary
    cd07642
    Location:34248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:9511006
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:582676
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:300406
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:309396
    PH; PH domain
    pfam01412
    Location:424541
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:610664
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:589621
    ANK; ANK repeat [structural motif]
  2. XM_006711902.1XP_006711965.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X8

    Conserved Domains (8) summary
    cd07642
    Location:34248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:906961
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:582676
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:300406
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:309396
    PH; PH domain
    pfam01412
    Location:424541
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:610664
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:589621
    ANK; ANK repeat [structural motif]
  3. XM_011510408.3XP_011508710.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X9

    Conserved Domains (7) summary
    cd00204
    Location:593687
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:311417
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:320407
    PH; PH domain
    pfam01412
    Location:435552
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:621675
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:600632
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34259
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  4. XM_011510405.3XP_011508707.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X4

    Conserved Domains (8) summary
    cd11966
    Location:937992
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:568662
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:286392
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:295382
    PH; PH domain
    pfam01412
    Location:410527
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:596650
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:575607
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34234
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  5. XM_011510403.3XP_011508705.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1

    Conserved Domains (8) summary
    cd11966
    Location:9621017
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:593687
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:311417
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:320407
    PH; PH domain
    pfam01412
    Location:435552
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:621675
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:600632
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34259
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  6. XM_011510407.2XP_011508709.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X6

    Conserved Domains (8) summary
    cd11966
    Location:917972
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:593687
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:311417
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:320407
    PH; PH domain
    pfam01412
    Location:435552
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:621675
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:600632
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34259
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  7. XM_011510404.2XP_011508706.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2

    Conserved Domains (8) summary
    cd11966
    Location:9591014
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:590684
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:308414
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:317404
    PH; PH domain
    pfam01412
    Location:432549
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:618672
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:597629
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34259
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  8. XM_011510406.2XP_011508708.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X5

    Conserved Domains (8) summary
    cd11966
    Location:920975
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:551645
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:269375
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:278365
    PH; PH domain
    pfam01412
    Location:393510
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:579633
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:558590
    ANK; ANK repeat [structural motif]
    cl12013
    Location:11217
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  9. XM_006711901.3XP_006711964.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X7

    Conserved Domains (8) summary
    cd11966
    Location:912967
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:543637
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:261367
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:270357
    PH; PH domain
    pfam01412
    Location:385502
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:571625
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:550582
    ANK; ANK repeat [structural motif]
    cl12013
    Location:3209
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
  10. XM_011510409.1XP_011508711.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X10

    Conserved Domains (8) summary
    cd11966
    Location:833888
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:464558
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:182288
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:191278
    PH; PH domain
    pfam01412
    Location:306423
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:492546
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:471503
    ANK; ANK repeat [structural motif]
    cl12013
    Location:1130
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
Support Center