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SLD2 Sld2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853752, updated on 18-Sep-2024

Summary

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Official Symbol
SLD2
Official Full Name
Sld2p
Primary source
SGD:S000001591
Locus tag
YKL108W
See related
AllianceGenome:SGD:S000001591; FungiDB:YKL108W; VEuPathDB:YKL108W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
DRC1
Summary
Enables DNA replication origin binding activity and single-stranded DNA binding activity. Involved in several processes, including DNA-dependent DNA replication; double-strand break repair via break-induced replication; and mitotic DNA replication checkpoint signaling. Located in cytoplasm and nucleus. Part of DNA replication preinitiation complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (234426..235787)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Kti12p Neighboring gene transcription factor HAP4 Neighboring gene putative short-chain dehydrogenase/reductase Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Transcriptional repression of CDC6 and SLD2 during meiosis is associated with production of short heterogeneous RNA isoforms. Phizicky DV, et al. Chromosoma, 2018 Dec. PMID 30276463, Free PMC Article
  2. Regulated eukaryotic DNA replication origin firing with purified proteins. Yeeles JT, et al. Nature, 2015 Mar 26. PMID 25739503, Free PMC Article
  3. The unstructured C-terminal tail of yeast Dpb11 (human TopBP1) protein is dispensable for DNA replication and the S phase checkpoint but required for the G2/M checkpoint. Navadgi-Patil VM, et al. J Biol Chem, 2011 Nov 25. PMID 21956112, Free PMC Article
  4. Sld2 binds to origin single-stranded DNA and stimulates DNA annealing. Kanter DM, et al. Nucleic Acids Res, 2011 Apr. PMID 21109535, Free PMC Article
  5. Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast. Zegerman P, et al. Nature, 2007 Jan 18. PMID 17167417

See all (56) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Repression of CDC6 and SLD2 during meiosis coincides with expression of shortened isoforms.
    Title: Transcriptional repression of CDC6 and SLD2 during meiosis is associated with production of short heterogeneous RNA isoforms.
  2. Dma1/2 mediate rapid Sld2 degradation by recognizing a complex phospho-degron
    Title: Robust Replication Control Is Generated by Temporal Gaps between Licensing and Firing Phases and Depends on Degradation of Firing Factor Sld2.
  3. These data suggest that Sld2 association with DNA is required for CMG assembly in S phase.
    Title: The replication initiation protein Sld2 regulates helicase assembly.
  4. origin ssDNA may stimulate the formation of the CMG complex by alleviating inhibitory interactions between Sld2 with Mcm2-7.
    Title: Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA.
  5. CDK promotes the formation of a newly identified fragile complex, the preloading complex (pre-LC) containing DNA polymerase epsilon (Pol epsilon), GINS, Sld2, and Dpb11
    Title: CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast.
  6. MSA1 is a high copy suppressor of a drc1-1 mutant.
    Title: Identification of MSA1, a cell cycle-regulated, dosage suppressor of drc1/sld2 and dpb11 mutants.
  7. Sld2 and Sld3 are the minimal set of cyclin-dependent kinase targets required for DNA replication
    Title: Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast.
  8. Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS
    Title: CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast.
  9. Phosphorylation of canonical motifs in Sld2 does not play a direct role in complex formation with Dpb11, but rather regulates phosphorylation of another residue, Thr84.
    Title: A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2-Dpb11.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA replication origin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA replication origin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA replication origin binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA replication initiation IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication initiation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in DNA replication preinitiation complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in double-strand break repair via break-induced replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in double-strand break repair via break-induced replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic DNA replication checkpoint signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic DNA replication preinitiation complex assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of protein-containing complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA replication preinitiation complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of DNA replication preinitiation complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of DNA replication preinitiation complex IGI
Inferred from Genetic Interaction
more info
 PubMed 
located_in cytoplasm HDA PubMed 
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Sld2p
NP_012814.1
  • Single-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    234426..235787
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179674.1 → NP_012814.1  TPA: Sld2p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012814.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VXH9, P34252
    UniProtKB/TrEMBL
    A6ZZK8, G2WHS5, N1P046
    Conserved Domains (1) summary
    cl26235
    Location:9 → 453
    RecQL4_SLD2_NTD; N-terminal homeodomain-like domain of metazoan RecQ protein-like 4 (RecQL4), fungal DNA replication regulator SLD2 and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P34252.1

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