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STE18 Ste18p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853548, updated on 18-Sep-2024

Summary

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Official Symbol
STE18
Official Full Name
Ste18p
Primary source
SGD:S000003846
Locus tag
YJR086W
See related
AllianceGenome:SGD:S000003846; FungiDB:YJR086W; VEuPathDB:YJR086W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables G-protein beta-subunit binding activity. Involved in pheromone-dependent signal transduction involved in conjugation with cellular fusion. Located in cytoplasm and plasma membrane. Part of Cdc24p-Far1p-Gbetagamma complex; G-protein beta/gamma-subunit complex; and heterotrimeric G-protein complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: X
Exon count:
1
Sequence:
Chromosome: X; NC_001142.9 (586068..586400)

Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Tmh11p Neighboring gene Emc2p Neighboring gene survivin

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The C-terminal tail preceding the CAAX box of a yeast G protein gamma subunit is dispensable for receptor-mediated G protein activation in vivo. Chinault SL, et al. J Biol Chem, 2003 Jun 6. PMID 12665529
  2. Biochemical and genetic analysis of dominant-negative mutations affecting a yeast G-protein gamma subunit. Grishin AV, et al. Mol Cell Biol, 1994 Jul. PMID 8007961, Free PMC Article
  3. Common modifications of trimeric G proteins and ras protein: involvement of polyisoprenylation. Finegold AA, et al. Science, 1990 Jul 13. PMID 1695391
  4. Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Membrane Association. Chernova TA, et al. Int J Mol Sci, 2020 Jul 16. PMID 32708832, Free PMC Article
  5. Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling. Fukuda N, et al. FEBS J, 2009 May. PMID 19476500

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Membrane Association.
    Title: Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Membrane Association.
  2. phosphorylation of the yeast Ggamma subunit (Ste18), together with Fus3 docking on Ste5, controls the rate and stability of Ste5/plasma membrane association.
    Title: Negative Feedback Phosphorylation of Gγ Subunit Ste18 and the Ste5 Scaffold Synergistically Regulates MAPK Activation in Yeast.
  3. Data show that the pheromone-responsive G protein subunits beta/gamma (Gbetagamma; STE4/STE18) dimer promotes the polarization of the pheromone receptor by interacting with casein kinases 1 or 2 (Yck1/2) and locally inhibiting receptor phosphorylation.
    Title: Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.
  4. These results indicate that chemoattractant-induced cell polarization requires continuous receptor-Galphabetagamma communication but not modulation of MAP kinase signaling.
    Title: Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables G-protein beta-subunit binding IEA
Inferred from Electronic Annotation
more info
  
enables G-protein beta-subunit binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in G protein-coupled receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in G protein-coupled receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion IEA
Inferred from Electronic Annotation
more info
  
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to pheromone IEA
Inferred from Electronic Annotation
more info
  
involved_in signal transduction IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of Cdc24p-Far1p-Gbetagamma complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of G-protein beta/gamma-subunit complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of G-protein beta/gamma-subunit complex IGI
Inferred from Genetic Interaction
more info
 PubMed 
part_of G-protein beta/gamma-subunit complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cell periphery HDA PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
part_of heterotrimeric G-protein complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of heterotrimeric G-protein complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of heterotrimeric G-protein complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Ste18p
NP_012619.1
  • G protein gamma subunit; forms dimer with Ste4p to activate mating signaling pathway, heterotrimer with Gpa1p, Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling; N-terminal phosphorylation of Ste18p functions synergistically with Fus3p docking site on Ste5p to restrict Ste5p plasma membrane recruitment and signaling; combinatorial phosphorylation at S3 and S7, by pheromone and pH stress, modulates Ste18p structure, Fus3p activation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001142.9 Reference assembly

    Range
    586068..586400
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181743.1 → NP_012619.1  TPA: Ste18p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012619.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VWQ5, P18852
    UniProtKB/TrEMBL
    A6ZQ42, B3LQH1, C7GMC0, C8ZBN2, G2WH89, N1P161
    Conserved Domains (1) summary
    pfam00631
    Location:29 → 91
    G-gamma; GGL domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P18852.1

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