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PRP18 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852889, updated on 22-Aug-2024

Summary

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Official Symbol
PRP18
Primary source
FungiDB:YGR006W
Locus tag
YGR006W
See related
SGD:S000003238; AllianceGenome:SGD:S000003238; VEuPathDB:YGR006W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Contributes to second spliceosomal transesterification activity. Involved in generation of catalytic spliceosome for second transesterification step and nuclear retention of unspliced pre-mRNA at the site of transcription. Part of U2-type post-spliceosomal complex; U4/U6 x U5 tri-snRNP complex; and U5 snRNP. Orthologous to human PRPF18 (pre-mRNA processing factor 18). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (506070..506825)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene peroxisome biogenesis protein Neighboring gene transcription factor IIF subunit TFG2 Neighboring gene ethanolamine-phosphate cytidylyltransferase Neighboring gene ATPase-stabilizing factor family protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing. Crotti LB, et al. Genes Dev, 2007 May 15. PMID 17504938, Free PMC Article
  2. Crystal structure of the functional domain of the splicing factor Prp18. Jiang J, et al. Proc Natl Acad Sci U S A, 2000 Mar 28. PMID 10737784, Free PMC Article
  3. A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Horowitz DS, et al. Mol Cell Biol, 1993 May. PMID 8474454, Free PMC Article
  4. PRP18, a protein required for the second reaction in pre-mRNA splicing. Vijayraghavan U, et al. Mol Cell Biol, 1990 Jan. PMID 2403639, Free PMC Article
  5. Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18. Bacíková D, et al. RNA, 2002 Oct. PMID 12403466, Free PMC Article

See all (69) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
  2. structure along with sequence comparisons among SRA1p orthologs and against authentic RNA recognition motif (RRM) proteins indicates that it is not an RRM domain but rather an all-helical protein with a fold more similar to the PRP18 splicing factor
    Title: The C-terminal domain of SRA1p has a fold more similar to PRP18 than to an RRM and does not directly bind to the SRA1 RNA STR7 region.
  3. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing.
    Title: The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing.
  4. Arginine1753 of Prp8 collaborates with Prp18 to stabilize U5 snRNA/exon contacts that are important for the second transesterification step of pre-mRNA splicing.
    Title: Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.
  5. Prp18 acts in concert with U5 snRNA during the second step of pre-mRNA splicing.
    Title: Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
contributes_to second spliceosomal transesterification activity IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to second spliceosomal transesterification activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in generation of catalytic spliceosome for second transesterification step IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in generation of catalytic spliceosome for second transesterification step IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear mRNA surveillance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in nuclear mRNA surveillance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of U2-type post-spliceosomal complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of U2-type post-spliceosomal complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of U4/U6 x U5 tri-snRNP complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of U4/U6 x U5 tri-snRNP complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of U5 snRNP IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of U5 snRNP IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of spliceosomal complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
mRNA splicing protein PRP18
NP_011520.2
  • Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    506070..506825
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181135.1 → NP_011520.2  TPA: mRNA splicing protein PRP18 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011520.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VUE3, P33411, Q02459, Q05724
    UniProtKB/TrEMBL
    A6ZUZ8, B3LIL0, C7GK76, C8Z8Q0, G2WE99, N1P217
    Conserved Domains (1) summary
    pfam02840
    Location:116 → 243
    Prp18; Prp18 domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P33411.3

Gene LinkOut

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