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VPS33 tethering complex ATP-binding subunit VPS33 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851112, updated on 18-Sep-2024

Summary

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Official Symbol
VPS33
Official Full Name
tethering complex ATP-binding subunit VPS33
Primary source
SGD:S000004388
Locus tag
YLR396C
See related
AllianceGenome:SGD:S000004388; FungiDB:YLR396C; VEuPathDB:YLR396C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
CLS14; MET27; PEP14; SLP1; VAM5; VPL25; VPT33
Summary
Enables ATP binding activity. Contributes to phosphatidylinositol binding activity. Involved in several processes, including Golgi to vacuole transport; piecemeal microautophagy of the nucleus; and vacuole fusion. Located in cytosol and fungal-type vacuole membrane. Part of CORVET complex and HOPS complex. Human ortholog(s) of this gene implicated in arthrogryposis multiplex congenita; arthrogryposis, renal dysfunction, and cholestasis 1; cholestasis; and kidney disease. Orthologous to human VPS33A (VPS33A core subunit of CORVET and HOPS complexes) and VPS33B (VPS33B late endosome and lysosome associated). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (910235..912310, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene SUMO ligase CST9 Neighboring gene cytochrome c oxidase subunit VIII Neighboring gene AAA family ATPase AFG2 Neighboring gene SKI complex RNA helicase subunit SKI2

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Intracellular distribution of amino acids in an slp1 vacuole-deficient mutant of the yeast Saccharomyces cerevisiae. Gent DP, et al. J Appl Microbiol, 1998 May. PMID 9674128
  2. The vacuolar compartment is required for sulfur amino acid homeostasis in Saccharomyces cerevisiae. Jacquemin-Faure I, et al. Mol Gen Genet, 1994 Sep 1. PMID 8078479
  3. Mutants of Saccharomyces cerevisiae with defective vacuolar function. Kitamoto K, et al. J Bacteriol, 1988 Jun. PMID 3131305, Free PMC Article
  4. Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. Banta LM, et al. Mol Cell Biol, 1990 Sep. PMID 2201898, Free PMC Article
  5. The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function. Wada Y, et al. Mol Cell Biol, 1990 May. PMID 2183024, Free PMC Article

See all (128) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Thus, Vps33/HOPS promotes productive SNARE assembly in the presence of otherwise inhibitory Sec17.
    Title: Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.
  2. Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes.
    Title: The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane.
  3. Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes
    Title: SM proteins Sly1 and Vps33 co-assemble with Sec17 and SNARE complexes to oppose SNARE disassembly by Sec18.
  4. Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.
    Title: A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.
  5. Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified.
    Title: The Habc domain of the SNARE Vam3 interacts with the HOPS tethering complex to facilitate vacuole fusion.
  6. we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association
    Title: Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.
  7. contribution of Vps33 to hemifusion and pore opening between vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing.
    Title: The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.
  8. Vps33p functions at multiple trafficking steps and is not limited to action at the vacuolar membrane
    Title: The Sec1/Munc18 protein, Vps33p, functions at the endosome and the vacuole of Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
contributes_to phosphatidylinositol binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in Golgi to vacuole transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular protein transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in piecemeal microautophagy of the nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein targeting to vacuole IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of SNARE complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in vacuole fusion, non-autophagic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vacuole organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vesicle docking IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in vesicle fusion with vacuole IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in vesicle tethering IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in vesicle-mediated transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of CORVET complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of CORVET complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of HOPS complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in fungal-type vacuole IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in fungal-type vacuole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in fungal-type vacuole membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in fungal-type vacuole membrane IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
tethering complex ATP-binding subunit VPS33
NP_013500.3
  • ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    910235..912310 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182284.3 → NP_013500.3  TPA: tethering complex ATP-binding subunit VPS33 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013500.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VZ31, P20795
    UniProtKB/TrEMBL
    A7A1S1, B3RHR1, G2WJL1, N1P1U4
    Conserved Domains (1) summary
    COG5158
    Location:14 → 651
    SEC1; Proteins involved in synaptic transmission and general secretion, Sec1 family [Intracellular trafficking and secretion]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P20795.1

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