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MCM8 minichromosome maintenance 8 homologous recombination repair factor [ Homo sapiens (human) ]

Gene ID: 84515, updated on 3-Mar-2026
Official Symbol
MCM8provided by HGNC
Official Full Name
minichromosome maintenance 8 homologous recombination repair factorprovided by HGNC
Primary source
HGNC:HGNC:16147
See related
Ensembl:ENSG00000125885 MIM:608187; AllianceGenome:HGNC:16147
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
POF10; C20orf154; dJ967N21.5
Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
Expression
Broad expression in testis (RPKM 3.9), lymph node (RPKM 1.8) and 24 other tissues See more
Orthologs
all
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Try the new Transcripts and proteins table
See MCM8 in Genome Data Viewer
Location:
20p12.3
Exon count:
21
Annotation release Status Assembly Chr Location
RS_2025_08 current GRCh38.p14 (GCF_000001405.40) 20 NC_000020.11 (5950652..5998977)
RS_2025_08 current T2T-CHM13v2.0 (GCF_009914755.1) 20 NC_060944.1 (5990987..6039282)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 20 NC_000020.10 (5931298..5979623)

Chromosome 20 - NC_000020.11Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 12660 Neighboring gene KN motif and ankyrin repeat domains 1 pseudogene 1 Neighboring gene ReSE screen-validated silencer GRCh37_chr20:5920579-5920767 Neighboring gene tRNA methyltransferase 6 non-catalytic subunit Neighboring gene ATAC-STARR-seq lymphoblastoid active region 17533 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr20:5950104-5950670 Neighboring gene MCM8 antisense RNA 1 Neighboring gene H3K27ac hESC enhancer GRCh37_chr20:5986511-5987012 Neighboring gene RNA, 7SL, cytoplasmic 498, pseudogene Neighboring gene Sharpr-MPRA regulatory region 14083 Neighboring gene cardiolipin synthase 1

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Tissue-specific circular RNA induction during human fetal development
  • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
  • BioProject: PRJNA270632
  • Publication: PMID 26076956
  • Analysis date: Mon Apr 2 22:54:59 2018

Related articles in PubMed

  1. Genetic markers of ovarian follicle number and menopause in women of multiple ethnicities. Schuh-Huerta SM, et al. Hum Genet, 2012 Nov. PMID 22696150, Free PMC Article
  2. Two novel mutations in the MCM8 gene shared by two Chinese siblings with primary ovarian insufficiency and short stature. Wang F, et al. Mol Genet Genomic Med, 2020 Sep. PMID 32652893, Free PMC Article
  3. Germline biallelic Mcm8 variants are associated with early-onset Lynch-like syndrome. Golubicki M, et al. JCI Insight, 2020 Sep 17. PMID 32841224, Free PMC Article
  4. Identification of mini-chromosome maintenance 8 as a potential prognostic marker and its effects on proliferation and apoptosis in gastric cancer. Huang B, et al. J Cell Mol Med, 2020 Dec. PMID 33155430, Free PMC Article
  5. Crystal structure of the winged-helix domain of MCM8. Zeng H, et al. Biochem Biophys Res Commun, 2020 Jun 11. PMID 32295713

See all (86) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MCM8 promotes lung cancer progression through upregulating DNAJC10.
    Title: MCM8 promotes lung cancer progression through upregulating DNAJC10.
  2. Understanding the novel MCM8 gene mutation: primary ovarian insufficiency and uterine hypoplasia in siblings.
    Title: Understanding the novel MCM8 gene mutation: primary ovarian insufficiency and uterine hypoplasia in siblings.
  3. MCM8 promotes gastric cancer progression through RPS15A and predicts poor prognosis.
    Title: MCM8 promotes gastric cancer progression through RPS15A and predicts poor prognosis.
  4. MCM8 is regulated by EGFR signaling and promotes the growth of glioma stem cells through its interaction with DNA-replication-initiating factors.
    Title: MCM8 is regulated by EGFR signaling and promotes the growth of glioma stem cells through its interaction with DNA-replication-initiating factors.
  5. A novel cancer-germline gene DAZL promotes progression and cisplatin resistance of non-small cell lung cancer by upregulating JAK2 and MCM8.
    Title: A novel cancer-germline gene DAZL promotes progression and cisplatin resistance of non-small cell lung cancer by upregulating JAK2 and MCM8.
  6. Mechanism of DNA unwinding by MCM8-9 in complex with HROB.
    Title: Mechanism of DNA unwinding by MCM8-9 in complex with HROB.
  7. Activity, substrate preference and structure of the HsMCM8/9 helicase.
    Title: Activity, substrate preference and structure of the HsMCM8/9 helicase.
  8. Enhancer-driven transcription of MCM8 by E2F4 promotes ATR pathway activation and glioma stem cell characteristics.
    Title: Enhancer-driven transcription of MCM8 by E2F4 promotes ATR pathway activation and glioma stem cell characteristics.
  9. Structural study of the N-terminal domain of human MCM8/9 complex.
    Title: Structural study of the N-terminal domain of human MCM8/9 complex.
  10. Associations of MCM8 rs3761873 and rs16991617 variants with abnormal uterine bleeding induced by copper intrauterine device.
    Title: Associations of MCM8 rs3761873 and rs16991617 variants with abnormal uterine bleeding induced by copper intrauterine device.
Submit: New GeneRIF Correction See all GeneRIFs (37)

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description Tests
Premature ovarian failure 10
MedGen: C4225402 OMIM: 612885 GeneReviews: Not available
Compare labs

EBI GWAS Catalog

Description
A genome-wide association study of early menopause and the combined impact of identified variants.
EBI GWAS Catalog
Genome-wide association studies identify loci associated with age at menarche and age at natural menopause.
EBI GWAS Catalog
Genome-wide association study of pre-eclampsia detects novel maternal single nucleotide polymorphisms and copy-number variants in subsets of the Hyperglycemia and Adverse Pregnancy Outcome (HAPO) study cohort.
EBI GWAS Catalog
Host determinants of HIV-1 control in African Americans.
EBI GWAS Catalog
Loci at chromosomes 13, 19 and 20 influence age at natural menopause.
EBI GWAS Catalog
Meta-analyses identify 13 loci associated with age at menopause and highlight DNA repair and immune pathways.
EBI GWAS Catalog

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Clone Names

  • MGC4816, MGC12866, MGC119522, MGC119523

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables 3'-5' DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3'-5' DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables MutLbeta complex binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables MutSalpha complex binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables MutSbeta complex binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables enzyme binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to single-stranded DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in DNA damage response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
NOT involved_in DNA replication IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in double-strand break repair via homologous recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in double-strand break repair via homologous recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in double-strand break repair via homologous recombination NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in female gamete generation IEA
Inferred from Electronic Annotation
more info
  
involved_in female gamete generation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in male gamete generation IEA
Inferred from Electronic Annotation
more info
  
involved_in male gamete generation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein localization to chromatin IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein stabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in recombinational interstrand cross-link repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of MCM complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of MCM8-MCM9 complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of MCM8-MCM9 complex IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrial matrix IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
DNA helicase MCM8
Names
DNA 3'-5' helicase MCM8
DNA replication licensing factor MCM8
MCM8 minichromosome maintenance deficient 8
REC homolog
minichromosome maintenance complex component 8
NP_001268449.1
NP_001268450.1
NP_001268451.1
NP_115874.3
NP_877954.1
XP_016883594.1
XP_047296508.1
XP_047296509.1
XP_047296510.1
XP_047296511.1
XP_054180111.1
XP_054180112.1
XP_054180113.1
XP_054180114.1
XP_054180115.1

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_042869.2 RefSeqGene

    Range
    5002..53327
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001281520.2 → NP_001268449.1  DNA helicase MCM8 isoform 1

    See identical proteins and their annotated locations for NP_001268449.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1).
    Source sequence(s)
    AJ439063, AL035461, BC101057
    Consensus CDS
    CCDS13094.1
    UniProtKB/Swiss-Prot
    B2RBG7, D3DW08, E7EQU7, Q495R4, Q495R6, Q495R7, Q86US4, Q969I5, Q9UJA3
    UniProtKB/TrEMBL
    A0A494C100
    Related
    ENSP00000368174.3, ENST00000378896.7
    Conserved Domains (1) summary
    COG1241
    Location:139 → 837
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]

  2. NM_001281521.2 → NP_001268450.1  DNA helicase MCM8 isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an in-frame exon and uses an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is longer, compared to isoform 1.
    Source sequence(s)
    AJ439063, AL035461, BC008830, BC101055
    Consensus CDS
    CCDS63227.1
    UniProtKB/TrEMBL
    A0A494C100
    Related
    ENSP00000368164.2, ENST00000378886.6
    Conserved Domains (3) summary
    cd00009
    Location:475 → 633
    AAA; The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, ...
    COG1241
    Location:139 → 877
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
    pfam14551
    Location:98 → 223
    MCM_N; MCM N-terminal domain

  3. NM_001281522.2 → NP_001268451.1  DNA helicase MCM8 isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1.
    Source sequence(s)
    AJ439063, AK297806, AL035461, BC008830
    Consensus CDS
    CCDS63226.1
    UniProtKB/Swiss-Prot
    Q9UJA3
    UniProtKB/TrEMBL
    B4DN82
    Related
    ENSP00000368161.1, ENST00000378883.5
    Conserved Domains (2) summary
    COG1241
    Location:139 → 790
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
    pfam14551
    Location:98 → 223
    MCM_N; MCM N-terminal domain

  4. NM_032485.6 → NP_115874.3  DNA helicase MCM8 isoform 1

    See identical proteins and their annotated locations for NP_115874.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes isoform (1). Both variants 1 and 3 encode the same isoform.
    Source sequence(s)
    AJ439063, AK297806, AL035461, BC008830
    Consensus CDS
    CCDS13094.1
    UniProtKB/Swiss-Prot
    B2RBG7, D3DW08, E7EQU7, Q495R4, Q495R6, Q495R7, Q86US4, Q969I5, Q9UJA3
    UniProtKB/TrEMBL
    A0A494C100
    Related
    ENSP00000478141.1, ENST00000610722.4
    Conserved Domains (1) summary
    COG1241
    Location:139 → 837
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]

  5. NM_182802.3 → NP_877954.1  DNA helicase MCM8 isoform 2

    See identical proteins and their annotated locations for NP_877954.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the internal coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.
    Source sequence(s)
    AJ439063, AK297806, AL035461, AY158211
    Consensus CDS
    CCDS13095.1
    UniProtKB/Swiss-Prot
    Q9UJA3
    UniProtKB/TrEMBL
    B4DN82
    Related
    ENSP00000265187.4, ENST00000265187.4
    Conserved Domains (3) summary
    cd00009
    Location:419 → 577
    AAA; The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, ...
    COG1241
    Location:139 → 821
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
    pfam14551
    Location:98 → 223
    MCM_N; MCM N-terminal domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2025_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000020.11 Reference GRCh38.p14 Primary Assembly

    Range
    5950652..5998977
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047440552.1 → XP_047296508.1  DNA helicase MCM8 isoform X2

  2. XM_017028105.2 → XP_016883594.1  DNA helicase MCM8 isoform X1

    UniProtKB/TrEMBL
    A0A494C100
    Conserved Domains (3) summary
    cd00009
    Location:475 → 633
    AAA; The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, ...
    COG1241
    Location:139 → 877
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
    pfam14551
    Location:98 → 223
    MCM_N; MCM N-terminal domain

  3. XM_047440553.1 → XP_047296509.1  DNA helicase MCM8 isoform X3

  4. XM_047440555.1 → XP_047296511.1  DNA helicase MCM8 isoform X5

  5. XM_047440554.1 → XP_047296510.1  DNA helicase MCM8 isoform X4

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060944.1 Alternate T2T-CHM13v2.0

    Range
    5990987..6039282
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054324137.1 → XP_054180112.1  DNA helicase MCM8 isoform X2

  2. XM_054324136.1 → XP_054180111.1  DNA helicase MCM8 isoform X1

    UniProtKB/TrEMBL
    A0A494C100

  3. XM_054324138.1 → XP_054180113.1  DNA helicase MCM8 isoform X3

  4. XM_054324140.1 → XP_054180115.1  DNA helicase MCM8 isoform X5

  5. XM_054324139.1 → XP_054180114.1  DNA helicase MCM8 isoform X4

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9UJA3.2 GenPept UniProtKB/Swiss-Prot:Q9UJA3

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