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Mff mitochondrial fission factor [ Mus musculus (house mouse) ]

Gene ID: 75734, updated on 9-Mar-2023

Summary

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Official Symbol
Mffprovided by MGI
Official Full Name
mitochondrial fission factorprovided by MGI
Primary source
MGI:MGI:1922984
See related
Ensembl:ENSMUSG00000026150 AllianceGenome:MGI:1922984
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
5230400G24Rik
Summary
Predicted to enable protein homodimerization activity. Involved in mitochondrial fission and protein targeting to mitochondrion. Located in mitochondrial outer membrane and peroxisome. Orthologous to human MFF (mitochondrial fission factor). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in CNS E18 (RPKM 63.2), cerebellum adult (RPKM 48.6) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Mff in Genome Data Viewer
Location:
1; 1 C5
Exon count:
12
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (82702599..82730111)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (82724890..82752390)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene collagen, type IV, alpha 4 Neighboring gene collagen, type IV, alpha 3 Neighboring gene RIKEN cDNA C430014B12 gene Neighboring gene transmembrane 4 L six family member 20 Neighboring gene predicted gene, 19257

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. MFF-dependent mitochondrial fission regulates presynaptic release and axon branching by limiting axonal mitochondria size. Lewis TL Jr, et al. Nat Commun, 2018 Nov 27. PMID 30479337, Free PMC Article
  2. Removal of the Mitochondrial Fission Factor Mff Exacerbates Neuronal Loss and Neurological Phenotypes in a Huntington's Disease Mouse Model. Cha MY, et al. PLoS Curr, 2018 Jul 26. PMID 30279997, Free PMC Article
  3. Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate. Ducommun S, et al. Cell Signal, 2015 May. PMID 25683918
  4. Mitochondrial fission factor (Mff) is required for organization of the mitochondrial sheath in spermatids. Varuzhanyan G, et al. Biochim Biophys Acta Gen Subj, 2021 May. PMID 33476744, Free PMC Article
  5. MAVS is energized by Mff which senses mitochondrial metabolism via AMPK for acute antiviral immunity. Hanada Y, et al. Nat Commun, 2020 Nov 11. PMID 33177519, Free PMC Article

See all (40) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mitochondrial fission factor (Mff) is required for organization of the mitochondrial sheath in spermatids.
    Title: Mitochondrial fission factor (Mff) is required for organization of the mitochondrial sheath in spermatids.
  2. MAVS is energized by Mff which senses mitochondrial metabolism via AMPK for acute antiviral immunity.
    Title: MAVS is energized by Mff which senses mitochondrial metabolism via AMPK for acute antiviral immunity.
  3. the results identify Sirt1 as a protective factor, which inhibits the JNK/Mff/mitochondrial fission pathway and sustains Factin homeostasis, and has potential implications for novel approaches to diabetic vasculopathy.
    Title: Sirt1 inhibits HG-induced endothelial injury: Role of Mff-based mitochondrial fission and F‑actin homeostasis-mediated cellular migration.
  4. the Drp1 'receptor' Mitochondrial fission factor (MFF) is required for determining the size of mitochondria entering the axon.
    Title: MFF-dependent mitochondrial fission regulates presynaptic release and axon branching by limiting axonal mitochondria size.
  5. By facilitating Mff-mediated mitochondrial fission and FUNDC1-required mitophagy, NR4A1 disturbed mitochondrial homeostasis, enhanced endothelial apoptosis and provoked microvascular dysfunction in myocardial ischemia reperfusion injury.
    Title: NR4A1 aggravates the cardiac microvascular ischemia reperfusion injury through suppressing FUNDC1-mediated mitophagy and promoting Mff-required mitochondrial fission by CK2α.
  6. The cardiac ischemia/reperfusion injury - induced DUSP1 deficiency promoted the activation of JNK which upregulated the expression of the mitochondrial fission factor (Mff).
    Title: DUSP1 alleviates cardiac ischemia/reperfusion injury by suppressing the Mff-required mitochondrial fission and Bnip3-related mitophagy via the JNK pathways.
  7. mitochondrial fission factor
    Title: Mff-Dependent Mitochondrial Fission Contributes to the Pathogenesis of Cardiac Microvasculature Ischemia/Reperfusion Injury via Induction of mROS-Mediated Cardiolipin Oxidation and HK2/VDAC1 Disassociation-Involved mPTP Opening.
  8. MiD51 can suppress Mff-dependent enhancement of Drp1 GTPase activity.
    Title: Cooperative and independent roles of the Drp1 adaptors Mff, MiD49 and MiD51 in mitochondrial fission.
  9. Mff cannot bind to assembly-deficient mutants of Drp1, suggesting that Mff selectively interacts with higher-order complexes of Drp1.
    Title: The mitochondrial fission receptor Mff selectively recruits oligomerized Drp1.
  10. These findings provide a mechanism wherein the multimeric states of both Mff and Drp1 regulate their collaborative interaction.
    Title: Dynamin-related Protein 1 Oligomerization in Solution Impairs Functional Interactions with Membrane-anchored Mitochondrial Fission Factor.
Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Endonuclease-mediated (3) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables GTPase binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein-containing complex binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in mitochondrial fission IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mitochondrial fission IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitochondrial fragmentation involved in apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in mitochondrial fusion ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within mitochondrion morphogenesis ISO
Inferred from Sequence Orthology
more info
  
involved_in peroxisome fission ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of cardiac muscle cell apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of mitochondrial fission IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in positive regulation of mitochondrial fission ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of protein targeting to membrane ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of release of cytochrome c from mitochondria ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of synaptic vesicle endocytosis ISO
Inferred from Sequence Orthology
more info
  
involved_in protein targeting to mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in protein targeting to mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein targeting to mitochondrion ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of mitochondrion organization ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of peroxisome organization ISO
Inferred from Sequence Orthology
more info
  
involved_in release of cytochrome c from mitochondria ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
colocalizes_with mitochondrial membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrial outer membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  
is_active_in peroxisome IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in peroxisome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in peroxisome ISO
Inferred from Sequence Orthology
more info
  
part_of protein-containing complex ISO
Inferred from Sequence Orthology
more info
  
located_in synapse IEA
Inferred from Electronic Annotation
more info
  
located_in synaptic vesicle ISO
Inferred from Sequence Orthology
more info
  
colocalizes_with synaptic vesicle membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
mitochondrial fission factor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001310695.1 → NP_001297624.1  mitochondrial fission factor isoform 2

    See identical proteins and their annotated locations for NP_001297624.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice junction and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AK017226, AU080386, BC059229
    Consensus CDS
    CCDS78630.1
    UniProtKB/Swiss-Prot
    Q6PCP5
    Related
    ENSMUSP00000072784.6, ENSMUST00000073025.12
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 239
    Miff; Mitochondrial and peroxisomal fission factor Mff

  2. NM_001310697.1 → NP_001297626.1  mitochondrial fission factor isoform 3

    See identical proteins and their annotated locations for NP_001297626.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AK139649, AU080386, BC059229
    Consensus CDS
    CCDS78631.1
    UniProtKB/Swiss-Prot
    Q6PCP5
    Related
    ENSMUSP00000125230.2, ENSMUST00000160786.8
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 238
    Miff; Mitochondrial and peroxisomal fission factor Mff

  3. NM_001310699.1 → NP_001297628.1  mitochondrial fission factor isoform 4

    See identical proteins and their annotated locations for NP_001297628.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AC138214, AU080386, BC059229, CX231761
    Consensus CDS
    CCDS78632.1
    UniProtKB/Swiss-Prot
    Q6PCP5
    Related
    ENSMUSP00000124200.2, ENSMUST00000160972.8
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 218
    Miff; Mitochondrial and peroxisomal fission factor Mff

  4. NM_001372405.1 → NP_001359334.1  mitochondrial fission factor isoform 5

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) represents the longest transcript and encodes the longest isoform (5).
    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:27 → 317
    Miff; Mitochondrial and peroxisomal fission factor Mff

  5. NM_001372406.1 → NP_001359335.1  mitochondrial fission factor isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 303
    Miff; Mitochondrial and peroxisomal fission factor Mff

  6. NM_001372407.1 → NP_001359336.1  mitochondrial fission factor isoform 7

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 302
    Miff; Mitochondrial and peroxisomal fission factor Mff

  7. NM_001372408.1 → NP_001359337.1  mitochondrial fission factor isoform 8

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 292
    Miff; Mitochondrial and peroxisomal fission factor Mff

  8. NM_001372409.1 → NP_001359338.1  mitochondrial fission factor isoform 9

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 272
    Miff; Mitochondrial and peroxisomal fission factor Mff

  9. NM_001372411.1 → NP_001359340.1  mitochondrial fission factor isoform 10

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 271
    Miff; Mitochondrial and peroxisomal fission factor Mff

  10. NM_001372412.1 → NP_001359341.1  mitochondrial fission factor isoform 11

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 250
    Miff; Mitochondrial and peroxisomal fission factor Mff

  11. NM_001372413.1 → NP_001359342.1  mitochondrial fission factor isoform 12

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Related
    ENSMUSP00000125223.2, ENSMUST00000160750.8
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 249
    Miff; Mitochondrial and peroxisomal fission factor Mff

  12. NM_001372414.1 → NP_001359343.1  mitochondrial fission factor isoform 13

    Status: VALIDATED

    Source sequence(s)
    AC138214
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 219
    Miff; Mitochondrial and peroxisomal fission factor Mff

  13. NM_029409.3 → NP_083685.2  mitochondrial fission factor isoform 1

    See identical proteins and their annotated locations for NP_083685.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes isoform 1.
    Source sequence(s)
    AU080386, BC059229
    Consensus CDS
    CCDS15098.1
    UniProtKB/Swiss-Prot
    Q6PCP5, Q9D3P5
    Related
    ENSMUSP00000077446.7, ENSMUST00000078332.13
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 291
    Miff; Mitochondrial and peroxisomal fission factor Mff

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    82702599..82730111
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036154165.1 → XP_036010058.1  mitochondrial fission factor isoform X1

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 354
    Miff; Mitochondrial and peroxisomal fission factor Mff

  2. XM_036154176.1 → XP_036010069.1  mitochondrial fission factor isoform X3

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 343
    Miff; Mitochondrial and peroxisomal fission factor Mff

  3. XM_036154189.1 → XP_036010082.1  mitochondrial fission factor isoform X9

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 323
    Miff; Mitochondrial and peroxisomal fission factor Mff

  4. XM_006496556.4 → XP_006496619.1  mitochondrial fission factor isoform X5

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 329
    Miff; Mitochondrial and peroxisomal fission factor Mff

  5. XM_006496558.3 → XP_006496621.1  mitochondrial fission factor isoform X11

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 318
    Miff; Mitochondrial and peroxisomal fission factor Mff

  6. XM_006496562.4 → XP_006496625.1  mitochondrial fission factor isoform X14

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 298
    Miff; Mitochondrial and peroxisomal fission factor Mff

  7. XM_006496564.4 → XP_006496627.1  mitochondrial fission factor isoform X17

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 276
    Miff; Mitochondrial and peroxisomal fission factor Mff

  8. XM_006496566.5 → XP_006496629.1  mitochondrial fission factor isoform X19

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 265
    Miff; Mitochondrial and peroxisomal fission factor Mff

  9. XM_006496568.5 → XP_006496631.1  mitochondrial fission factor isoform X21

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 245
    Miff; Mitochondrial and peroxisomal fission factor Mff

  10. XM_036154172.1 → XP_036010065.1  mitochondrial fission factor isoform X2

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 353
    Miff; Mitochondrial and peroxisomal fission factor Mff

  11. XM_036154179.1 → XP_036010072.1  mitochondrial fission factor isoform X4

    UniProtKB/TrEMBL
    A0A1S6GWG0
    Conserved Domains (1) summary
    pfam05644
    Location:27 → 342
    Miff; Mitochondrial and peroxisomal fission factor Mff

  12. XM_036154191.1 → XP_036010084.1  mitochondrial fission factor isoform X10

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 322
    Miff; Mitochondrial and peroxisomal fission factor Mff

  13. XM_006496557.5 → XP_006496620.1  mitochondrial fission factor isoform X6

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 328
    Miff; Mitochondrial and peroxisomal fission factor Mff

  14. XM_006496563.5 → XP_006496626.1  mitochondrial fission factor isoform X15

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 297
    Miff; Mitochondrial and peroxisomal fission factor Mff

  15. XM_006496565.5 → XP_006496628.1  mitochondrial fission factor isoform X18

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 275
    Miff; Mitochondrial and peroxisomal fission factor Mff

  16. XM_006496567.5 → XP_006496630.1  mitochondrial fission factor isoform X20

    See identical proteins and their annotated locations for XP_006496630.1

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 264
    Miff; Mitochondrial and peroxisomal fission factor Mff

  17. XM_006496569.4 → XP_006496632.1  mitochondrial fission factor isoform X22

    Conserved Domains (1) summary
    pfam05644
    Location:27 → 244
    Miff; Mitochondrial and peroxisomal fission factor Mff

  18. XM_036154185.1 → XP_036010078.1  mitochondrial fission factor isoform X7

    Conserved Domains (1) summary
    pfam05644
    Location:1 → 328
    Miff; Mitochondrial and peroxisomal fission factor Mff

  19. XM_036154192.1 → XP_036010085.1  mitochondrial fission factor isoform X12

    Conserved Domains (1) summary
    pfam05644
    Location:1 → 317
    Miff; Mitochondrial and peroxisomal fission factor Mff

  20. XM_036154187.1 → XP_036010080.1  mitochondrial fission factor isoform X8

    Conserved Domains (1) summary
    pfam05644
    Location:1 → 327
    Miff; Mitochondrial and peroxisomal fission factor Mff

  21. XM_036154194.1 → XP_036010087.1  mitochondrial fission factor isoform X13

    UniProtKB/TrEMBL
    E0CYB9
    Related
    ENSMUSP00000124334.2, ENSMUST00000162003.8
    Conserved Domains (1) summary
    pfam05644
    Location:1 → 316
    Miff; Mitochondrial and peroxisomal fission factor Mff

  22. XM_036154199.1 → XP_036010092.1  mitochondrial fission factor isoform X16

    Conserved Domains (1) summary
    pfam05644
    Location:1 → 296
    Miff; Mitochondrial and peroxisomal fission factor Mff
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6PCP5.1 GenPept UniProtKB/Swiss-Prot:Q6PCP5

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