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Oplah 5-oxoprolinase (ATP-hydrolysing) [ Mus musculus (house mouse) ]

Gene ID: 75475, updated on 2-Nov-2024

Summary

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Official Symbol
Oplahprovided by MGI
Official Full Name
5-oxoprolinase (ATP-hydrolysing)provided by MGI
Primary source
MGI:MGI:1922725
See related
Ensembl:ENSMUSG00000022562 AllianceGenome:MGI:1922725
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
5-OPase; 1700010G02Rik
Summary
Predicted to enable 5-oxoprolinase (ATP-hydrolyzing) activity and identical protein binding activity. Predicted to be involved in glutathione metabolic process. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; cranium; genitourinary system; lung; and thymus. Orthologous to human OPLAH (5-oxoprolinase, ATP-hydrolysing). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Broad expression in kidney adult (RPKM 41.9), adrenal adult (RPKM 38.5) and 24 other tissues See more
Orthologs
human all
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Genomic context

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See Oplah in Genome Data Viewer
Location:
15 D3; 15 35.67 cM
Exon count:
30
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (76180803..76215381, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (76296603..76331181, complement)

Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene spermatogenesis and centriole associated 1 Neighboring gene myocardial infraction associated transcript 2 Neighboring gene microRNA 3079 Neighboring gene sphingomyelin phosphodiesterase 5 Neighboring gene STARR-seq mESC enhancer starr_39050 Neighboring gene STARR-positive B cell enhancer ABC_E7384 Neighboring gene exosome component 4 Neighboring gene GPI anchor attachment protein 1 Neighboring gene ribosomal protein S6, pseudogene 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. OPLAH ablation leads to accumulation of 5-oxoproline, oxidative stress, fibrosis, and elevated fillings pressures: a murine model for heart failure with a preserved ejection fraction. van der Pol A, et al. Cardiovasc Res, 2018 Dec 1. PMID 30032247
  2. Accumulation of 5-oxoproline in myocardial dysfunction and the protective effects of OPLAH. van der Pol A, et al. Sci Transl Med, 2017 Nov 8. PMID 29118264
  3. Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer. Lu W, et al. Anal Chem, 2010 Apr 15. PMID 20349993, Free PMC Article
  4. A futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase: the cause of cellular ATP depletion in nephrotic cystinosis? Kumar A, et al. J Biosci, 2010 Mar. PMID 20413906
  5. Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue. Duteil D, et al. Cell Rep, 2016 Oct 18. PMID 27760309, Free PMC Article

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Oplah full body knock-out mice had higher 5-oxoproline levels coupled to increased oxidative stress, myocardial fibrosis and elevated filling pressures reminiscent of heart failure with preserved ejection fraction.
    Title: OPLAH ablation leads to accumulation of 5-oxoproline, oxidative stress, fibrosis, and elevated fillings pressures: a murine model for heart failure with a preserved ejection fraction.
  2. OPLAH depletion occurred as a result of cardiac injury, leading to elevated 5-oxoproline and oxidative stress, whereas OPLAH overexpression improved cardiac function after ischemic injury.
    Title: Accumulation of 5-oxoproline in myocardial dysfunction and the protective effects of OPLAH.
  3. the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase
    Title: A futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase: the cause of cellular ATP depletion in nephrotic cystinosis?
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 
  • Targeted (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Clone Names

  • MGC28663, MGC36824

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 5-oxoprolinase (ATP-hydrolyzing) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 5-oxoprolinase (ATP-hydrolyzing) activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in glutathione metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within glutathione metabolic process ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
5-oxoprolinase
Names
5-oxo-L-prolinase
pyroglutamase
NP_694762.1
XP_006521570.1
XP_006521571.1
XP_006521572.1
XP_030104666.1
XP_030104667.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_153122.3 → NP_694762.1  5-oxoprolinase

    See identical proteins and their annotated locations for NP_694762.1

    Status: VALIDATED

    Source sequence(s)
    AC155074
    Consensus CDS
    CCDS27565.1
    UniProtKB/Swiss-Prot
    Q8K010, Q8R3K2
    Related
    ENSMUSP00000023222.7, ENSMUST00000023222.13
    Conserved Domains (1) summary
    PLN02666
    Location:1 → 1258
    PLN02666; 5-oxoprolinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000081.7 Reference GRCm39 C57BL/6J

    Range
    76180803..76215381 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006521509.3 → XP_006521572.1  5-oxoprolinase isoform X3

    See identical proteins and their annotated locations for XP_006521572.1

    UniProtKB/Swiss-Prot
    Q8K010, Q8R3K2
    Conserved Domains (1) summary
    PLN02666
    Location:1 → 1258
    PLN02666; 5-oxoprolinase

  2. XM_030248806.2 → XP_030104666.1  5-oxoprolinase isoform X3

    UniProtKB/Swiss-Prot
    Q8K010, Q8R3K2
    Conserved Domains (1) summary
    PLN02666
    Location:1 → 1258
    PLN02666; 5-oxoprolinase

  3. XM_006521508.5 → XP_006521571.1  5-oxoprolinase isoform X2

    Conserved Domains (1) summary
    PLN02666
    Location:41 → 1298
    PLN02666; 5-oxoprolinase

  4. XM_006521507.5 → XP_006521570.1  5-oxoprolinase isoform X1

    Conserved Domains (2) summary
    PLN02666
    Location:97 → 1354
    PLN02666; 5-oxoprolinase
    pfam15058
    Location:1 → 138
    Speriolin_N; Speriolin N-terminus

  5. XM_030248807.2 → XP_030104667.1  5-oxoprolinase isoform X4

    Conserved Domains (1) summary
    PLN02666
    Location:17 → 1134
    PLN02666; 5-oxoprolinase

RNA

  1. XR_384054.5 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8K010.1 GenPept UniProtKB/Swiss-Prot:Q8K010

Gene LinkOut

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