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Spire1 spire type actin nucleation factor 1 [ Mus musculus (house mouse) ]

Gene ID: 68166, updated on 2-Nov-2024

Summary

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Official Symbol
Spire1provided by MGI
Official Full Name
spire type actin nucleation factor 1provided by MGI
Primary source
MGI:MGI:1915416
See related
Ensembl:ENSMUSG00000024533 AllianceGenome:MGI:1915416
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Spir-1; 6030430B19Rik
Summary
Predicted to enable microtubule binding activity. Involved in several processes, including cytoskeleton organization; cytoskeleton-dependent cytokinesis; and intracellular transport. Acts upstream of or within Golgi vesicle transport; behavioral fear response; and dendritic spine development. Located in Golgi apparatus; cell cortex; and cytoplasmic vesicle membrane. Colocalizes with cleavage furrow. Is expressed in several structures, including central nervous system; genitourinary system; intestine; liver; and spleen. Orthologous to human SPIRE1 (spire type actin nucleation factor 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Broad expression in cerebellum adult (RPKM 16.5), frontal lobe adult (RPKM 16.3) and 22 other tissues See more
Orthologs
human all
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Genomic context

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See Spire1 in Genome Data Viewer
Location:
18 E1; 18 39.99 cM
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 18 NC_000084.7 (67621279..67745880, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 18 NC_000084.6 (67488209..67612810, complement)

Chromosome 18 - NC_000084.7Genomic Context describing neighboring genes Neighboring gene AFG3-like AAA ATPase 2 Neighboring gene STARR-positive B cell enhancer ABC_E3234 Neighboring gene PRELI domain containing 3A Neighboring gene STARR-seq mESC enhancer starr_44904 Neighboring gene STARR-seq mESC enhancer starr_44905 Neighboring gene STARR-seq mESC enhancer starr_44907 Neighboring gene predicted gene, 17669 Neighboring gene STARR-positive B cell enhancer mm9_chr18:67771962-67772263 Neighboring gene centrosomal protein 76 Neighboring gene proteasome (prosome, macropain) assembly chaperone 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Lnc-SMaRT Translational Regulation of Spire1, A New Player in Muscle Differentiation. Scalzitti S, et al. J Mol Biol, 2022 Jan 30. PMID 34863993
  2. Spire localization via zinc finger-containing domain is crucial for the asymmetric division of mouse oocyte. Jo YJ, et al. FASEB J, 2019 Mar. PMID 30557038
  3. Spire-1 contributes to the invadosome and its associated invasive properties. Lagal V, et al. J Cell Sci, 2014 Jan 15. PMID 24213528
  4. Expression patterns of the mouse Spir-2 actin nucleator. Pleiser S, et al. Gene Expr Patterns, 2010 Oct-Dec. PMID 20723621
  5. Two mechanisms drive pronuclear migration in mouse zygotes. Scheffler K, et al. Nat Commun, 2021 Feb 5. PMID 33547291, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lnc-SMaRT Translational Regulation of Spire1, A New Player in Muscle Differentiation.
    Title: Lnc-SMaRT Translational Regulation of Spire1, A New Player in Muscle Differentiation.
  2. Two mechanisms drive pronuclear migration in mouse zygotes.
    Title: Two mechanisms drive pronuclear migration in mouse zygotes.
  3. After injection of double-strand RNA against Spire 1 and 2 (Spire1+2 double-strand RNA), actin mesh levels were significantly decreased compared with that of control (negative).
    Title: Spire localization via zinc finger-containing domain is crucial for the asymmetric division of mouse oocyte.
  4. Spire-1 is specifically recruited at invadosomes and is part of a multi-molecular complex containing Src kinase, the formin mDia1 and actin.
    Title: Spire-1 contributes to the invadosome and its associated invasive properties.
  5. analysis of the molecular basis of the Spir1/formin-2 interaction
    Title: Molecular basis of actin nucleation factor cooperativity: crystal structure of the Spir-1 kinase non-catalytic C-lobe domain (KIND)•formin-2 formin SPIR interaction motif (FSI) complex.
  6. Spire1 and Spire2 drive two critical steps of asymmetric oocyte division.
    Title: Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
  7. By analyzing the expression of the spir-1 gene it was shown that spir-1 and formin-2 have a nearly identical expression pattern during mouse embryogenesis and in the adult brain.
    Title: Overlapping expression pattern of the actin organizers Spir-1 and formin-2 in the developing mouse nervous system and the adult brain.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 
  • Gene trapped (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables actin binding IEA
Inferred from Electronic Annotation
more info
  
enables microtubule binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in Golgi vesicle transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within Golgi vesicle transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in actin cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin cytoskeleton organization ISO
Inferred from Sequence Orthology
more info
  
involved_in actin filament network formation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin filament polymerization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin nucleation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within behavioral fear response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cleavage furrow formation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cleavage furrow formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within dendritic spine development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment of meiotic spindle localization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in establishment of meiotic spindle localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment of meiotic spindle localization ISO
Inferred from Sequence Orthology
more info
  
involved_in formin-nucleated actin cable assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in formin-nucleated actin cable assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in intracellular transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in polar body extrusion after meiotic divisions IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in polar body extrusion after meiotic divisions IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of double-strand break repair ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of mitochondrial fission ISO
Inferred from Sequence Orthology
more info
  
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in vesicle-mediated transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cell cortex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell cortex IDA
Inferred from Direct Assay
more info
 PubMed 
colocalizes_with cleavage furrow IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasmic vesicle membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasmic vesicle membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial outer membrane ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
protein spire homolog 1
Names
spire homolog 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001403659.1 → NP_001390588.1  protein spire homolog 1 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC120410

  2. NM_001403660.1 → NP_001390589.1  protein spire homolog 1 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC120410

  3. NM_001403661.1 → NP_001390590.1  protein spire homolog 1 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC120410
    Related
    ENSMUSP00000080871.7, ENSMUST00000082243.7

  4. NM_176832.2 → NP_789802.1  protein spire homolog 1 isoform 2

    See identical proteins and their annotated locations for NP_789802.1

    Status: VALIDATED

    Source sequence(s)
    AC120410
    Consensus CDS
    CCDS37849.1
    UniProtKB/Swiss-Prot
    Q52KF3, Q6PDJ5, Q80V45
    UniProtKB/TrEMBL
    A0A286YDN9
    Related
    ENSMUSP00000153492.2, ENSMUST00000224799.2
    Conserved Domains (1) summary
    cd15767
    Location:347 → 467
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins

  5. NM_194355.3 → NP_919336.2  protein spire homolog 1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC120410
    Conserved Domains (2) summary
    cd15767
    Location:620 → 740
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    pfam16474
    Location:24 → 223
    KIND; Kinase non-catalytic C-lobe domain

RNA

  1. NR_175397.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC120410

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000084.7 Reference GRCm39 C57BL/6J

    Range
    67621279..67745880 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006526206.2 → XP_006526269.1  protein spire homolog 1 isoform X4

    UniProtKB/Swiss-Prot
    Q52KF3, Q6PDJ5, Q80V45
    UniProtKB/TrEMBL
    A0A5H1ZRL1
    Related
    ENSMUSP00000110702.4, ENSMUST00000115050.10
    Conserved Domains (2) summary
    cd15767
    Location:575 → 695
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    pfam16474
    Location:24 → 223
    KIND; Kinase non-catalytic C-lobe domain

  2. XM_006526203.5 → XP_006526266.1  protein spire homolog 1 isoform X2

    Conserved Domains (2) summary
    cd15767
    Location:633 → 753
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    pfam16474
    Location:24 → 223
    KIND; Kinase non-catalytic C-lobe domain

  3. XM_006526209.5 → XP_006526272.1  protein spire homolog 1 isoform X1

    Conserved Domains (2) summary
    cd15767
    Location:634 → 754
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    pfam16474
    Location:24 → 223
    KIND; Kinase non-catalytic C-lobe domain

  4. XM_030250590.2 → XP_030106450.1  protein spire homolog 1 isoform X7

    UniProtKB/Swiss-Prot
    Q52KF3, Q6PDJ5, Q80V45
    UniProtKB/TrEMBL
    D3YTL8
    Related
    ENSMUSP00000049336.7, ENSMUST00000045105.13
    Conserved Domains (2) summary
    cd15767
    Location:475 → 595
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    cl21453
    Location:1 → 78
    PKc_like; Protein Kinases, catalytic domain

  5. XM_030250587.2 → XP_030106447.1  protein spire homolog 1 isoform X6

    UniProtKB/Swiss-Prot
    Q52KF3, Q6PDJ5, Q80V45
    Conserved Domains (2) summary
    cd15767
    Location:489 → 609
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    cl21453
    Location:1 → 78
    PKc_like; Protein Kinases, catalytic domain

  6. XM_030250586.2 → XP_030106446.1  protein spire homolog 1 isoform X6

    UniProtKB/Swiss-Prot
    Q52KF3, Q6PDJ5, Q80V45
    Conserved Domains (2) summary
    cd15767
    Location:489 → 609
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    cl21453
    Location:1 → 78
    PKc_like; Protein Kinases, catalytic domain

  7. XM_030250589.2 → XP_030106449.1  protein spire homolog 1 isoform X7

    UniProtKB/Swiss-Prot
    Q52KF3, Q6PDJ5, Q80V45
    UniProtKB/TrEMBL
    D3YTL8
    Conserved Domains (2) summary
    cd15767
    Location:475 → 595
    FYVE_SPIR1; FYVE-related domain found in protein spire homolog 1 (Spire1) and similar proteins
    cl21453
    Location:1 → 78
    PKc_like; Protein Kinases, catalytic domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q52KF3.1 GenPept UniProtKB/Swiss-Prot:Q52KF3

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