klar klarsicht [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: klarprovided by FlyBase
Official Full Name: klarsichtprovided by FlyBase
Primary source: FLYBASE:FBgn0001316
Locus tag: Dmel_CG17046
See related: AllianceGenome:FB:FBgn0001316
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG17046; Dmel\CG17046; Klar; kls; marb
Summary: Enables kinesin binding activity. Involved in several processes, including larval visceral muscle development; lipid droplet transport along microtubule; and lumen formation, open tracheal system. Located in nuclear outer membrane and perinuclear region of cytoplasm. Colocalizes with cytoplasmic microtubule and lipid droplet. Is expressed in several structures, including embryonic primordium of adult salivary gland; embryonic/larval nervous system; extended germ band embryo; eye disc; and germline cyst. Used to study Emery-Dreifuss muscular dystrophy. [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 61C1-61C3; 3-0.5 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (434083..540614, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (434083..540614, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

loss of Bocksbeutel reduced Klarsicht localization to the nucleus and resulted in a disruption in nuclear separation. Loss of Otefin increased the transcription of Klarsicht and led to premature separation of nuclei and their positioning closer to the edge of the muscle.
Title: Drosophila emerins control LINC complex localization and transcription to regulate myonuclear position.
Data indicate that mushroom body defect protein (Mud) co-localized with centromeres, Klarsicht protein and Klaroid protein.
Title: Microtubule-driven nuclear rotations promote meiotic chromosome dynamics.
Two novel isoformes of Klar delta and/or epsilon are widely expressed during development, including in the embryonic and larval nervous system as well as in ovaries.
Title: Novel isoforms of the transport regulator klar.
A new mutant allele of Klar is described with a lesion specifically in the LD domain; this lesion abolishes both droplet localization of Klar and the ability of Klar to regulate droplet motion.
Title: Targeting the motor regulator Klar to lipid droplets.
Msp-300 has a function in egg laying, normally redundant with a function of Klar.
Title: On the roles of the Drosophila KASH domain proteins Msp-300 and Klarsicht.
expressed during oogenesis and localizes to the nuclear envelope of the germ line nuclei
Title: The Drosophila KASH domain proteins Msp-300 and Klarsicht and the SUN domain protein Klaroid have no essential function during oogenesis.
Klarsicht expression is limited to differentiating cells in the eye and may be part of the trigger for apical nuclear migration.
Title: Drosophila klaroid encodes a SUN domain protein required for Klarsicht localization to the nuclear envelope and nuclear migration in the eye.
Links the nucleus to the microtubule organizing center.
Title: Drosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye.
Klar isoforms control motion and positioning of multiple organelles
Title: Organelle-specific control of intracellular transport: distinctly targeted isoforms of the regulator Klar.
klar plays a role in salivary tube development in Drosophila
Title: Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1974 (e-PCR)
- UniSTS:44291

Dm0921 (e-PCR)
- UniSTS:75175

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables kinesin binding	IBA Inferred from Biological aspect of Ancestor more info
enables kinesin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye photoreceptor development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in flight	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in larval visceral muscle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid droplet transport along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lumen formation, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule anchoring	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of pole plasm oskar mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleus localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleus organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleus organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of intracellular transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tube morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with lipid droplet	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear outer membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in perinuclear region of cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: klarsicht

Names: CG17046-PA; CG17046-PB; CG17046-PC; CG17046-PD; CG17046-PE; CG17046-PF; CG17046-PG; CG17046-PH; CG17046-PI; klar-PA; klar-PB; klar-PC; klar-PD; klar-PE; klar-PF; klar-PG; klar-PH; klar-PI; klarischt; marbles

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

434083..540614 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001043111.3 → NP_001036576.1 klarsicht, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036576.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9Y0E4

UniProtKB/TrEMBL

Q7YU15

Conserved Domains (1) summary

pfam10541
Location:1992 → 2045

KASH; Nuclear envelope localization domain
NM_001274282.2 → NP_001261211.1 klarsicht, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261211.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9Y0E4

UniProtKB/TrEMBL

M9PGK7

Conserved Domains (1) summary

pfam10541
Location:2219 → 2272

KASH; Nuclear envelope localization domain
NM_079149.5 → NP_523873.2 klarsicht, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523873.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9Y0E4

UniProtKB/TrEMBL

Q9W0Q4

Related

FBpp0072464

Conserved Domains (1) summary

pfam10541
Location:2209 → 2262

KASH; Nuclear envelope localization domain
NM_001274283.2 → NP_001261212.1 klarsicht, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261212.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PBH0

Conserved Domains (1) summary

pfam10541
Location:1583 → 1636

KASH; Nuclear envelope localization domain
NM_001274284.1 → NP_001261213.1 klarsicht, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261213.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PDJ0

Conserved Domains (1) summary

pfam10541
Location:1127 → 1180

KASH; Nuclear envelope localization domain
NM_001103992.2 → NP_001097462.1 klarsicht, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097462.1

Status: REVIEWED

UniProtKB/TrEMBL

A8JNH1

Conserved Domains (1) summary

pfam10541
Location:514 → 567

KASH; Nuclear envelope localization domain
NM_001299960.1 → NP_001286889.1 klarsicht, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286889.1

Status: REVIEWED

UniProtKB/TrEMBL

X2JAH4

Conserved Domains (1) summary

pfam10541
Location:458 → 511

KASH; Nuclear envelope localization domain
NM_001103993.2 → NP_001097463.1 klarsicht, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097463.1

Status: REVIEWED

UniProtKB/TrEMBL

A8JNH2

Conserved Domains (1) summary

cl02488
Location:254 → 339

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
NM_001299959.1 → NP_001286888.1 klarsicht, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286888.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J8G9

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A8JNH1		A8JNH1
A8JNH2		A8JNH2
M9PBH0		M9PBH0
M9PDJ0		M9PDJ0
M9PGK7		M9PGK7
Q7YU15		Q7YU15
Q95S58		Q95S58
Q9W0Q4		Q9W0Q4
Q9Y0E4.1		Q9Y0E4
X2J8G9		X2J8G9
X2JAH4		X2JAH4