Adh4 alcohol dehydrogenase 4 (class II), pi polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adh4provided by MGI
Official Full Name: alcohol dehydrogenase 4 (class II), pi polypeptideprovided by MGI
Primary source: MGI:MGI:1349472
See related: Ensembl:ENSMUSG00000037797 AllianceGenome:MGI:1349472
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Adh2
Summary: Enables NADPH:quinone reductase activity; alcohol dehydrogenase (NAD+) activity; and aldose reductase (NADPH) activity. Involved in fatty acid omega-oxidation. Acts upstream of or within several processes, including alcohol catabolic process; ethanol metabolic process; and quinone metabolic process. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Is expressed in several structures, including adrenal cortex; genitourinary system; integumental system; limb; and nervous system. Human ortholog(s) of this gene implicated in alcohol dependence. Orthologous to human ADH4 (alcohol dehydrogenase 4 (class II), pi polypeptide). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Biased expression in liver adult (RPKM 28.8), testis adult (RPKM 3.3) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 G3; 3 64.24 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (138121217..138138444)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (138415456..138432683)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study demonistrated that adh1/adh4 knockout mice as possible rodent models for presymptomatic Parkinson's disease.
Title: Adh1 and Adh1/4 knockout mice as possible rodent models for presymptomatic Parkinson's disease.
The results suggested that lack of Adh4 gene activity induces changes in the function of the dopamine system, findings which are compatible with a role of loss-of-function mutations in ADH4 as possible risk factors for parkinson disease.
Title: Modeling Parkinson's disease genetics: altered function of the dopamine system in Adh4 knockout mice.
Studies of kinetic properties of mouse ADH2 in new variants with substitutions at position 47 find that the pK values are not lowered by Pro47 to His substitution, suggesting that His47 does not act as a catalytic base in deprotonation of the zinc ligand.
Title: Enzymatic mechanism of low-activity mouse alcohol dehydrogenase 2.
The metabolism of all-trans- and 9-cis-retinol/ retinaldehyde has been investigated with focus on the activities of human, mouse and rat alcohol dehydrogenase 2 (ADH2), an intriguing enzyme with apparently different functions in human and rodents.
Title: Alcohol dehydrogenase 2 is a major hepatic enzyme for human retinol metabolism.
Results describe the expression patterns of alcohol dehydrogenases I, III, and IV in adult rat, mouse and human tissues using radioactive oligonucleotide in situ hybridization.
Title: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain.
Adh4 metabolizes the omega-oxidation of 20-HETE in cerebral microvascular smooth muscle and endothelium.
Title: Omega-oxidation of 20-hydroxyeicosatetraenoic acid (20-HETE) in cerebral microvascular smooth muscle and endothelium by alcohol dehydrogenase 4.
Cellular expression of Adh4 in the GI tract was studied.
Title: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract: implications for Parkinson's disease.
Distinct retinoid metabolic functions for alcohol dehydrogenase genes Adh1 and Adh4 in protection against vitamin A toxicity or deficiency revealed in double null mutant mice
Title: Distinct retinoid metabolic functions for alcohol dehydrogenase genes Adh1 and Adh4 in protection against vitamin A toxicity or deficiency revealed in double null mutant mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Adh4 (e-PCR), detects polymorphism
- UniSTS:506715

Adh4 (e-PCR), detects polymorphism
- UniSTS:186487

NoName (e-PCR)
- UniSTS:489181

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables NAD binding	ISO Inferred from Sequence Orthology more info
enables S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity	IBA Inferred from Biological aspect of Ancestor more info
enables alcohol dehydrogenase (NAD+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables alcohol dehydrogenase (NAD+) activity	IDA Inferred from Direct Assay more info	PubMed
enables alcohol dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables alcohol dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info
enables aldose reductase (NADPH) activity	IDA Inferred from Direct Assay more info	PubMed
enables all-trans retinal binding	IEA Inferred from Electronic Annotation more info
enables all-trans retinal binding	ISO Inferred from Sequence Orthology more info
enables all-trans-retinol dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables all-trans-retinol dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info
enables all-trans-retinol dehydrogenase (NAD+) activity	ISS Inferred from Sequence or Structural Similarity more info
enables benzaldehyde dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables benzaldehyde dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables quinone reductase (NADPH) activity	IDA Inferred from Direct Assay more info	PubMed
enables retinol binding	IEA Inferred from Electronic Annotation more info
enables retinol binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within alcohol catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in alcohol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in alcohol metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within aldehyde metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in aldehyde metabolic process	IEA Inferred from Electronic Annotation more info
involved_in aldehyde metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ethanol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fatty acid omega-oxidation	IDA Inferred from Direct Assay more info	PubMed
involved_in fatty acid omega-oxidation	IEA Inferred from Electronic Annotation more info
involved_in fatty acid omega-oxidation	ISO Inferred from Sequence Orthology more info
involved_in formaldehyde catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within quinone metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in retinoid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in retinoid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in retinol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in retinol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in retinol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in small molecule catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: all-trans-retinol dehydrogenase [NAD(+)] ADH4

Names: alcohol dehydrogenase 2; alcohol dehydrogenase II; alcohol dehydrogenase class II; class II type ADH

NP_036126.2

EC 1.1.1.105

XP_030108487.1

EC 1.1.1.105

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011996.2 → NP_036126.2 all-trans-retinol dehydrogenase [NAD(+)] ADH4

See identical proteins and their annotated locations for NP_036126.2

Status: PROVISIONAL

Source sequence(s)

AK132994

Consensus CDS

CCDS38653.1

UniProtKB/Swiss-Prot

Q3V0P5, Q9QYY9

UniProtKB/TrEMBL

Q496S1

Related

ENSMUSP00000013458.9, ENSMUST00000013458.9

Conserved Domains (1) summary

cd08299
Location:3 → 377

alcohol_DH_class_I_II_IV; class I, II, IV alcohol dehydrogenases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

138121217..138138444

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252627.1 → XP_030108487.1 all-trans-retinol dehydrogenase [NAD(+)] ADH4 isoform X1

UniProtKB/TrEMBL

Q496S1

Conserved Domains (1) summary

cd08299
Location:18 → 389

alcohol_DH_class_I_II_IV; class I, II, IV alcohol dehydrogenases