Cntrob centrobin, centrosomal BRCA2 interacting protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cntrobprovided by MGI
Official Full Name: centrobin, centrosomal BRCA2 interacting proteinprovided by MGI
Primary source: MGI:MGI:2443290
See related: Ensembl:ENSMUSG00000032782 AllianceGenome:MGI:2443290
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lip8; Nip2; 9830165K03Rik
Summary: Predicted to enable protein domain specific binding activity. Predicted to be involved in centriole replication; centrosome separation; and mitotic cytokinetic process. Predicted to act upstream of or within cell division. Predicted to be located in cytosol. Predicted to be active in centriole and centrosome. Is expressed in early conceptus and testis. Orthologous to human CNTROB (centrobin, centriole duplication and spindle assembly protein). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 14.8), thymus adult (RPKM 11.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B3; 11 42.51 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (69188735..69214720, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (69297909..69323898, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cytoplasmic centrobin has roles in noncentrosomal microtubule formation in specific cell types and during the cell cycle
Title: The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm.
Centrobin may be a possible substrate of Nek2 responsible for the meiotic spindle assembly in the mouse oocytes.
Title: Nek2 and its substrate, centrobin/Nip2, are required for proper meiotic spindle formation of the mouse oocytes.
Data show that a residual amount of centrobin was detected at the centrosomes of the resting cells, suggesting its role for maintaining integrity of the centrosome, especially of the daughter centriole in the cells.
Title: Centrobin/Nip2 expression in vivo suggests its involvement in cell proliferation.
The phenotypic similarities of the Nip2/centrobin- and Nek2-suppressed embryos suggest that Nip2/centrobin may be a substrate of Nek2 that is required for mitotic spindle assembly in early mouse embryos.
Title: Nip2/centrobin may be a substrate of Nek2 that is required for proper spindle assembly during mitosis in early mouse embryos.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)

General gene information

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Markers

Bnip2 (e-PCR), detects polymorphism
- UniSTS:532248

D11Ertd172e (e-PCR), detects polymorphism
- UniSTS:167044

NoName (e-PCR)
- UniSTS:493045

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in centriole replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in centriole replication	ISO Inferred from Sequence Orthology more info
involved_in centriole replication	ISS Inferred from Sequence or Structural Similarity more info
involved_in centrosome separation	IBA Inferred from Biological aspect of Ancestor more info
involved_in centrosome separation	ISO Inferred from Sequence Orthology more info
involved_in centrosome separation	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitotic cytokinetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cytokinetic process	ISO Inferred from Sequence Orthology more info
involved_in mitotic cytokinetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cilium assembly	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in centriole	IBA Inferred from Biological aspect of Ancestor more info
located_in centriole	ISO Inferred from Sequence Orthology more info
located_in centriole	ISS Inferred from Sequence or Structural Similarity more info
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: centrobin

Names: LYST-interacting protein 8; centrosomal BRCA2-interacting protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_172560.3 → NP_766148.2 centrobin

See identical proteins and their annotated locations for NP_766148.2

Status: VALIDATED

Source sequence(s)

AK036710, AK147774, AL645527

Consensus CDS

CCDS24888.2

UniProtKB/Swiss-Prot

B0QZN4, Q5NCF3, Q8CB62

Related

ENSMUSP00000090651.6, ENSMUST00000092973.6

Conserved Domains (5) summary

cd14686
Location:366 → 422

bZIP; coiled coil [structural motif]

pfam15619
Location:199 → 374

Lebercilin; Ciliary protein causing Leber congenital amaurosis disease

cd16269
Location:368 → 379

GBP_C; coiled coil [structural motif]

cl20817
Location:297 → 411

GBP_C; Guanylate-binding protein, C-terminal domain

cl21462
Location:363 → 425

bZIP; Basic leucine zipper (bZIP) domain of bZIP transcription factors: a DNA-binding and dimerization domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

69188735..69214720 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156561.1 → XP_036012454.1 centrobin isoform X3

Conserved Domains (2) summary

PHA03247
Location:568 → 813

PHA03247; large tegument protein UL36; Provisional

COG1196
Location:176 → 554

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036156562.1 → XP_036012455.1 centrobin isoform X4

Conserved Domains (2) summary

PHA03247
Location:443 → 637

PHA03247; large tegument protein UL36; Provisional

COG1196
Location:179 → 447

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_011248918.3 → XP_011247220.1 centrobin isoform X2

Conserved Domains (2) summary

PHA03247
Location:571 → 816

PHA03247; large tegument protein UL36; Provisional

COG1196
Location:179 → 557

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_011248916.3 → XP_011247218.1 centrobin isoform X1

See identical proteins and their annotated locations for XP_011247218.1

Conserved Domains (2) summary

PHA03247
Location:571 → 765

PHA03247; large tegument protein UL36; Provisional

COG1196
Location:179 → 557

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036156563.1 → XP_036012456.1 centrobin isoform X6

Conserved Domains (2) summary

PHA03247
Location:285 → 479

PHA03247; large tegument protein UL36; Provisional

TIGR02168
Location:3 → 284

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_017314454.3 → XP_017169943.1 centrobin isoform X5

Conserved Domains (2) summary

PHA03247
Location:387 → 581

PHA03247; large tegument protein UL36; Provisional

COG1196
Location:71 → 385

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036156565.1 → XP_036012458.1 centrobin isoform X7

Conserved Domains (2) summary

PHA03247
Location:259 → 453

PHA03247; large tegument protein UL36; Provisional

COG1196
Location:10 → 263

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036156564.1 → XP_036012457.1 centrobin isoform X6

Conserved Domains (2) summary

PHA03247
Location:285 → 479

PHA03247; large tegument protein UL36; Provisional

TIGR02168
Location:3 → 284

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_006532878.5 → XP_006532941.1 centrobin isoform X6

Conserved Domains (2) summary

PHA03247
Location:285 → 479

PHA03247; large tegument protein UL36; Provisional

TIGR02168
Location:3 → 284

SMC_prok_B; chromosome segregation protein SMC, common bacterial type