Sphk1 sphingosine kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sphk1provided by MGI
Official Full Name: sphingosine kinase 1provided by MGI
Primary source: MGI:MGI:1316649
See related: Ensembl:ENSMUSG00000061878 AllianceGenome:MGI:1316649
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sk1; Spk1; 1110006G24Rik
Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Expression: Broad expression in lung adult (RPKM 8.3), duodenum adult (RPKM 6.9) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 E2; 11 81.42 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (116421662..116427502)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (116530836..116536675)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

An inducible sphingosine kinase 1 in hepatic stellate cells potentiates liver fibrosis.
Title: An inducible sphingosine kinase 1 in hepatic stellate cells potentiates liver fibrosis.
Long non-coding RNA LncTUG1 regulates favourable compression force-induced cementocytes mineralization via PU.1/TLR4/SphK1 signalling.
Title: Long non-coding RNA LncTUG1 regulates favourable compression force-induced cementocytes mineralization via PU.1/TLR4/SphK1 signalling.
miR-495 promotes intestinal epithelial cell apoptosis through downregulation of Sphingosine-1-phosphate.
Title: miR-495 promotes intestinal epithelial cell apoptosis through downregulation of Sphingosine-1-phosphate.
SphK1 deficiency ameliorates the development of atherosclerosis by inhibiting the S1P/S1PR3/Rhoa/ROCK pathway.
Title: SphK1 deficiency ameliorates the development of atherosclerosis by inhibiting the S1P/S1PR3/Rhoa/ROCK pathway.
Inhibition of sphingosine kinase 1 attenuates LPS-induced acute lung injury by suppressing endothelial cell pyroptosis.
Title: Inhibition of sphingosine kinase 1 attenuates LPS-induced acute lung injury by suppressing endothelial cell pyroptosis.
Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular carcinoma progression by disrupting glycolytic energy supply that drives tumor angiogenesis.
Title: Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular carcinoma progression by disrupting glycolytic energy supply that drives tumor angiogenesis.
UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1.
Title: UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1.
ATF3 regulates SPHK1 in cardiomyocyte injury via endoplasmic reticulum stress.
Title: ATF3 regulates SPHK1 in cardiomyocyte injury via endoplasmic reticulum stress.
The SphK1/S1P Axis Regulates Synaptic Vesicle Endocytosis via TRPC5 Channels.
Title: The SphK1/S1P Axis Regulates Synaptic Vesicle Endocytosis via TRPC5 Channels.
miR-542-5p targets c-myc and negates the cell proliferation effect of SphK1 in intestinal epithelial cells.
Title: miR-542-5p targets c-myc and negates the cell proliferation effect of SphK1 in intestinal epithelial cells.

Submit: New GeneRIF Correction See all GeneRIFs (175)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Sphk1 (e-PCR), detects polymorphism
- UniSTS:496531

NoName (e-PCR)
- UniSTS:236783

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables D-erythro-sphingosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables D-erythro-sphingosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables D-erythro-sphingosine kinase activity	ISO Inferred from Sequence Orthology more info
enables D-erythro-sphingosine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables acetyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISS Inferred from Sequence or Structural Similarity more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein phosphatase 2A binding	IEA Inferred from Electronic Annotation more info
enables protein phosphatase 2A binding	ISO Inferred from Sequence Orthology more info
enables sphingosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables sphingosine kinase activity	ISO Inferred from Sequence Orthology more info
enables sphingosine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables sphingosine-1-phosphate receptor activity	IEA Inferred from Electronic Annotation more info
enables sphingosine-1-phosphate receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood vessel development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within brain development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in calcium-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to growth factor stimulus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cellular response to hydrogen peroxide	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to vascular endothelial growth factor stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to vascular endothelial growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cyclooxygenase pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of ceramide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell growth	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of fibroblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-17 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-17 production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of mitotic nuclear division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of non-canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of non-canonical NF-kappaB signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of p38MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of p38MAPK cascade	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of peptidyl-threonine phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of smooth muscle contraction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in protein acetylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of endocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of endocytosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of endosomal vesicle fusion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of interleukin-1 beta production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of microglial cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuroinflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuroinflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuroinflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of phagocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of tumor necrosis factor-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to tumor necrosis factor	ISO Inferred from Sequence Orthology more info
involved_in response to tumor necrosis factor	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within sphingolipid mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in sphingosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sphingosine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in sphingosine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in sphingosine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in sphingosine metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in sphingosine-1-phosphate receptor signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in axon	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated pit	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated pit	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in endocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	ISO Inferred from Sequence Orthology more info
located_in synaptic vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sphingosine kinase 1

Names: acetyltransferase SPHK1

NP_001165943.1

EC 2.7.1.91

NP_001165944.1

EC 2.7.1.91

NP_001165946.1

EC 2.7.1.91

NP_001359413.1

EC 2.7.1.91

NP_001359414.1

EC 2.7.1.91

NP_001359415.1

EC 2.7.1.91

NP_001359416.1

EC 2.7.1.91

NP_001359417.1

EC 2.7.1.91

NP_001359418.1

EC 2.7.1.91

NP_079643.2

EC 2.7.1.91

XP_006532732.1

EC 2.7.1.91

XP_006532735.1

EC 2.7.1.91

XP_017169887.1

EC 2.7.1.91

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001172472.2 → NP_001165943.1 sphingosine kinase 1 isoform 2

See identical proteins and their annotated locations for NP_001165943.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.

Source sequence(s)

AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

O88886, Q3U2E3, Q8CI15, Q91ZN3

Related

ENSMUSP00000101994.2, ENSMUST00000106386.8

Conserved Domains (1) summary

PLN02958
Location:11 → 344

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001172473.1 → NP_001165944.1 sphingosine kinase 1 isoform 2

See identical proteins and their annotated locations for NP_001165944.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.

Source sequence(s)

AK160900, AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

O88886, Q3U2E3, Q8CI15, Q91ZN3

Conserved Domains (1) summary

PLN02958
Location:11 → 344

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001172475.1 → NP_001165946.1 sphingosine kinase 1 isoform 3

See identical proteins and their annotated locations for NP_001165946.1

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. This isoform is also known as Sphk1a.

Source sequence(s)

AL645851

Consensus CDS

CCDS48986.1

UniProtKB/Swiss-Prot

Q8CI15

Related

ENSMUSP00000097775.4, ENSMUST00000100201.10

Conserved Domains (1) summary

PLN02958
Location:10 → 343

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001372484.1 → NP_001359413.1 sphingosine kinase 1 isoform 2

Status: REVIEWED

Source sequence(s)

AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

O88886, Q3U2E3, Q8CI15, Q91ZN3

Related

ENSMUSP00000101996.3, ENSMUST00000106388.9

Conserved Domains (1) summary

PLN02958
Location:11 → 344

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001372485.1 → NP_001359414.1 sphingosine kinase 1 isoform 4

Status: REVIEWED

Source sequence(s)

AL645851

Conserved Domains (1) summary

PLN02958
Location:1 → 267

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001372486.1 → NP_001359415.1 sphingosine kinase 1 isoform 2

Status: REVIEWED

Source sequence(s)

AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

O88886, Q3U2E3, Q8CI15, Q91ZN3

Related

ENSMUSP00000101995.3, ENSMUST00000106387.9

Conserved Domains (1) summary

PLN02958
Location:11 → 344

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001372487.1 → NP_001359416.1 sphingosine kinase 1 isoform 4

Status: REVIEWED

Source sequence(s)

AL645851

Conserved Domains (1) summary

PLN02958
Location:1 → 267

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001372488.1 → NP_001359417.1 sphingosine kinase 1 isoform 3

Status: REVIEWED

Source sequence(s)

AL645851

Consensus CDS

CCDS48986.1

Conserved Domains (1) summary

PLN02958
Location:10 → 343

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_001372489.1 → NP_001359418.1 sphingosine kinase 1 isoform 3

Status: REVIEWED

Source sequence(s)

AL645851

Consensus CDS

CCDS48986.1

Conserved Domains (1) summary

PLN02958
Location:10 → 343

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_025367.7 → NP_079643.2 sphingosine kinase 1 isoform 2

See identical proteins and their annotated locations for NP_079643.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.

Source sequence(s)

AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

O88886, Q3U2E3, Q8CI15, Q91ZN3

Related

ENSMUSP00000067865.7, ENSMUST00000063446.13

Conserved Domains (1) summary

PLN02958
Location:11 → 344

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

116421662..116427502

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006532672.4 → XP_006532735.1 sphingosine kinase 1 isoform X3

See identical proteins and their annotated locations for XP_006532735.1

UniProtKB/Swiss-Prot

Q8CI15

Conserved Domains (1) summary

PLN02958
Location:10 → 343

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
XM_006532669.5 → XP_006532732.1 sphingosine kinase 1 isoform X2

UniProtKB/TrEMBL

O88885

Conserved Domains (1) summary

PLN02958
Location:140 → 473

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
XM_017314398.2 → XP_017169887.1 sphingosine kinase 1 isoform X1

UniProtKB/TrEMBL

O88885

Conserved Domains (1) summary

PLN02958
Location:141 → 474

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_011451.3: Suppressed sequence

Description

NM_011451.3: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.