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Htr4 5 hydroxytryptamine (serotonin) receptor 4 [ Mus musculus (house mouse) ]

Gene ID: 15562, updated on 3-May-2025
Official Symbol
Htr4provided by MGI
Official Full Name
5 hydroxytryptamine (serotonin) receptor 4provided by MGI
Primary source
MGI:MGI:109246
See related
Ensembl:ENSMUSG00000026322 AllianceGenome:MGI:109246
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
5-HT4; 5HTR4; 5-HT-4; 5-HT<4L>
Summary
Enables G protein-coupled serotonin receptor activity. Involved in several processes, including maintenance of gastrointestinal epithelium; mucus secretion; and regulation of postsynapse assembly. Is active in glutamatergic synapse; plasma membrane; and postsynapse. Is expressed in several structures, including brain; gallbladder; genitourinary system; gut; and spinal cord. Used to study anorexia nervosa. Orthologous to human HTR4 (5-hydroxytryptamine receptor 4). [provided by Alliance of Genome Resources, May 2025]
Expression
Biased expression in bladder adult (RPKM 1.5), colon adult (RPKM 1.5) and 6 other tissues See more
Orthologs
human all
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See Htr4 in Genome Data Viewer
Location:
18 E1; 18 35.1 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 18 NC_000084.7 (62456618..62636098)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 18 NC_000084.6 (62323546..62503027)

Chromosome 18 - NC_000084.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_44769 Neighboring gene STARR-seq mESC enhancer starr_44770 Neighboring gene predicted gene 9949 Neighboring gene adrenergic receptor, beta 2 Neighboring gene predicted gene, 36124 Neighboring gene STARR-seq mESC enhancer starr_44772 Neighboring gene predicted gene, 36316 Neighboring gene X-linked lymphocyte-regulated 5C pseudogene Neighboring gene F-box protein 38 Neighboring gene STARR-positive B cell enhancer ABC_E967 Neighboring gene serine peptidase inhibitor, Kazal type 10 Neighboring gene STARR-seq mESC enhancer starr_44774 Neighboring gene serine peptidase inhibitor, Kazal type 7 (putative)

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Related articles in PubMed

  1. Dual role of serotonin in the pathogenesis of indomethacin-induced small intestinal ulceration: pro-ulcerogenic action via 5-HT3 receptors and anti-ulcerogenic action via 5-HT4 receptors. Kato S, et al. Pharmacol Res, 2012 Sep. PMID 22699012
  2. How Does the Brain Implement Adaptive Decision Making to Eat? Compan V, et al. J Neurosci, 2015 Oct 14. PMID 26468187, Free PMC Article
  3. Expression and function of 5-HT4 receptors in the mouse enteric nervous system. Liu M, et al. Am J Physiol Gastrointest Liver Physiol, 2005 Dec. PMID 16037544
  4. Adaptive changes in serotonin neurons of the raphe nuclei in 5-HT(4) receptor knock-out mouse. Conductier G, et al. Eur J Neurosci, 2006 Aug. PMID 16930432
  5. Activation of 5-HT4 receptors inhibits secretion of beta-amyloid peptides and increases neuronal survival. Cho S, et al. Exp Neurol, 2007 Jan. PMID 16978609

See all (81) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Activation of the 5-hydroxytryptamine 4 receptor ameliorates tight junction barrier dysfunction in the colon of type 1 diabetic mice.
    Title: Activation of the 5-hydroxytryptamine 4 receptor ameliorates tight junction barrier dysfunction in the colon of type 1 diabetic mice.
  2. Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine.
    Title: Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine.
  3. Serotonin 5-HT4 receptor boosts functional maturation of dendritic spines via RhoA-dependent control of F-actin.
    Title: Serotonin 5-HT(4) receptor boosts functional maturation of dendritic spines via RhoA-dependent control of F-actin.
  4. Findings of the present in vitro study enrich the understanding and provide insight roles of 5-HT4R in embryonic brain development by promoting the growth of hippocampal neurons.
    Title: Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice.
  5. Synaptic potentiation is mediated by 5-HT4 receptors and that systemic modulation of 5-HT4 receptor function can bidirectionally impact memory formation.
    Title: Hippocampal 5-HT Input Regulates Memory Formation and Schaffer Collateral Excitation.
  6. Impeding serotonergic signaling preferentially gates spike-timing-dependent long-term depression (t-LTD) at thalamostriatal synapses. This t-LTD requires dampened activity of the 5-HT4 receptor subtype.
    Title: Serotonergic Signaling Controls Input-Specific Synaptic Plasticity at Striatal Circuits.
  7. Gut microbiota-produced Tryptamine activates epithelial 5-HT4R serotonin receptor to increase cAMP level and drive colonic fluid secretion.
    Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
  8. Prefrontal cortex 5-HT4Rs mediate hypophagia following stress.
    Title: Adaptive Control of Dorsal Raphe by 5-HT4 in the Prefrontal Cortex Prevents Persistent Hypophagia following Stress.
  9. Knock down serotonin receptors 5-HTR3 and 5-HTR4 in neonatal cardiomyocytes resulted in significant increase of cell damage in response to hypoxia, and also led to alternation in heart beating.
    Title: 5-HTR3 and 5-HTR4 located on the mitochondrial membrane and functionally regulated mitochondrial functions.
  10. Study shows that in the murine gastrointestinal tract, activation of 5-HT4 receptors with prucalopride enhances cholinergic contractions, illustrating facilitation of myenteric cholinergic neurotransmission.
    Title: 5-HT(4) receptors facilitate cholinergic neurotransmission throughout the murine gastrointestinal tract.
Submit: New GeneRIF Correction See all GeneRIFs (45)

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (4) 
  • Targeted (4)  1 citation

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables G protein-coupled serotonin receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables G protein-coupled serotonin receptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables G protein-coupled serotonin receptor activity ISO
Inferred from Sequence Orthology
more info
  
enables G protein-coupled serotonin receptor activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables neurotransmitter receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables serotonin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables serotonin receptor activity IEA
Inferred from Electronic Annotation
more info
  
enables serotonin receptor activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in G protein-coupled receptor signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in G protein-coupled serotonin receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in adenylate cyclase-activating serotonin receptor signaling pathway ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in adenylate cyclase-inhibiting serotonin receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chemical synaptic transmission IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chemical synaptic transmission IEA
Inferred from Electronic Annotation
more info
  
involved_in gamma-aminobutyric acid signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in large intestinal transit IEA
Inferred from Electronic Annotation
more info
  
involved_in large intestinal transit ISO
Inferred from Sequence Orthology
more info
  
involved_in maintenance of gastrointestinal epithelium IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mucus secretion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of appetite IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of postsynapse assembly EXP
Inferred from Experiment
more info
 PubMed 
involved_in regulation of postsynapse assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of postsynapse assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in dendrite IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endosome membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in glutamatergic synapse EXP
Inferred from Experiment
more info
 PubMed 
is_active_in glutamatergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in membrane ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in plasma membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in postsynapse IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
5-hydroxytryptamine receptor 4

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001364956.1 → NP_001351885.1  5-hydroxytryptamine receptor 4 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC131714, AC152454
    Consensus CDS
    CCDS89255.1
    UniProtKB/TrEMBL
    A0A494BA82
    Related
    ENSMUSP00000157982.2, ENSMUST00000237114.2
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  2. NM_001364957.1 → NP_001351886.1  5-hydroxytryptamine receptor 4 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC131714, AC152454
    Consensus CDS
    CCDS89257.1
    Related
    ENSMUSP00000157967.2, ENSMUST00000238081.2
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  3. NM_001364958.1 → NP_001351887.1  5-hydroxytryptamine receptor 4 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC131714, AC152454
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  4. NM_001364959.1 → NP_001351888.1  5-hydroxytryptamine receptor 4 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC131714, AC152454
    Consensus CDS
    CCDS89256.1
    Related
    ENSMUSP00000157470.2, ENSMUST00000236691.2
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  5. NM_008313.4 → NP_032339.2  5-hydroxytryptamine receptor 4 isoform 1

    See identical proteins and their annotated locations for NP_032339.2

    Status: VALIDATED

    Source sequence(s)
    AC131714, AC152454
    Consensus CDS
    CCDS29291.1
    UniProtKB/Swiss-Prot
    O89003, O89004, P97288, Q3URB5, Q9R2A4
    Related
    ENSMUSP00000027560.7, ENSMUST00000027560.8
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000084.7 Reference GRCm39 C57BL/6J

    Range
    62456618..62636098
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030250347.2 → XP_030106207.1  5-hydroxytryptamine receptor 4 isoform X6

    Conserved Domains (2) summary
    cd14964
    Location:21 → 47
    7tm_GPCRs; TM helix 1 [structural motif]
    cl28897
    Location:20 → 118
    7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily

  2. XM_006525682.5 → XP_006525745.1  5-hydroxytryptamine receptor 4 isoform X3

    See identical proteins and their annotated locations for XP_006525745.1

    UniProtKB/TrEMBL
    A0A494BA82
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  3. XM_006525681.5 → XP_006525744.1  5-hydroxytryptamine receptor 4 isoform X3

    See identical proteins and their annotated locations for XP_006525744.1

    UniProtKB/TrEMBL
    A0A494BA82
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  4. XM_006525683.5 → XP_006525746.1  5-hydroxytryptamine receptor 4 isoform X4

    See identical proteins and their annotated locations for XP_006525746.1

    UniProtKB/Swiss-Prot
    P97288
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  5. XM_030250345.2 → XP_030106205.1  5-hydroxytryptamine receptor 4 isoform X2

    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  6. XM_011246848.4 → XP_011245150.1  5-hydroxytryptamine receptor 4 isoform X5

    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  7. XM_006525680.5 → XP_006525743.1  5-hydroxytryptamine receptor 4 isoform X2

    See identical proteins and their annotated locations for XP_006525743.1

    UniProtKB/Swiss-Prot
    P97288
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  8. XM_030250344.2 → XP_030106204.1  5-hydroxytryptamine receptor 4 isoform X1

    UniProtKB/Swiss-Prot
    O89003, O89004, P97288, Q3URB5, Q9R2A4
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  9. XM_030250343.2 → XP_030106203.1  5-hydroxytryptamine receptor 4 isoform X1

    UniProtKB/Swiss-Prot
    O89003, O89004, P97288, Q3URB5, Q9R2A4
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  10. XM_006525676.5 → XP_006525739.1  5-hydroxytryptamine receptor 4 isoform X1

    See identical proteins and their annotated locations for XP_006525739.1

    UniProtKB/Swiss-Prot
    O89003, O89004, P97288, Q3URB5, Q9R2A4
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

  11. XM_006525678.5 → XP_006525741.1  5-hydroxytryptamine receptor 4 isoform X1

    See identical proteins and their annotated locations for XP_006525741.1

    UniProtKB/Swiss-Prot
    O89003, O89004, P97288, Q3URB5, Q9R2A4
    Conserved Domains (1) summary
    cd15056
    Location:20 → 323
    7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P97288.3 GenPept UniProtKB/Swiss-Prot:P97288

Gene LinkOut

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