Cd3e CD3 antigen, epsilon polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cd3eprovided by MGI
Official Full Name: CD3 antigen, epsilon polypeptideprovided by MGI
Primary source: MGI:MGI:88332
See related: Ensembl:ENSMUSG00000032093 AllianceGenome:MGI:88332
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CD3; T3e; AI504783; CD3epsilon
Summary: Predicted to enable several functions, including SH3 domain binding activity; identical protein binding activity; and protein heterodimerization activity. Involved in nervous system development and positive regulation of cell adhesion. Acts upstream of or within several processes, including positive regulation of T cell activation; positive regulation of cytokine production; and regulation of signal transduction. Located in several cellular components, including dendritic spine; external side of plasma membrane; and immunological synapse. Part of alpha-beta T cell receptor complex. Is expressed in colon and hemolymphoid system. Human ortholog(s) of this gene implicated in immunodeficiency 18. Orthologous to human CD3E (CD3e molecule). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in thymus adult (RPKM 115.4), spleen adult (RPKM 18.7) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 9 A5.2; 9 24.84 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (44910033..44920961, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (44998735..45009663, complement)
Build 37.2	previous assembly	MGSCv37 (GCF_000001635.18)	9	NC_000075.5 (44806826..44817673, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The data show that CD3epsilon chain couples to Cbl via simultaneous binding of both proteins to Numb, thus mediating TCR degradation
Title: CD3ε recruits Numb to promote TCR degradation.
IL-17-producing CD3(bright) gammadelta T cells responded promptly and strongly to pneumococcal infection and during skin inflammation.
Title: CD3bright signals on γδ T cells identify IL-17A-producing Vγ6Vδ1+ T cells.
these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling.
Title: LAPTM5 promotes lysosomal degradation of intracellular CD3ζ but not of cell surface CD3ζ.
these results indicate that membrane association of the CD3epsilon signaling domain is required for optimal thymocyte development and peripheral T cell function.
Title: Membrane association of the CD3ε signaling domain is required for optimal T cell development and function.
These results suggest that proteasome-mediated degradation is involved in hypophosphorylated LAT and PLCgamma1 in Dow2-induced anergic T cells. The novel CD3-specific Ab, Dow2, may provide us with a unique tool for inducing immunosuppression
Title: Novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLCγ1 following T-cell stimulation.
that Nck recruitment to the TCR is fundamental to mount an efficient T cell response in vivo, and that the Nck-CD3epsilon interaction may represent a target for pharmacological modulation of the immune response.
Title: Relevance of Nck-CD3 epsilon interaction for T cell activation in vivo.
Structure of murine CD3epsilon complexed with the mitogenic anti-CD3epsilon antibody 2C11 enabled structural comparisons of antibody-liganded and unliganded forms of CD3epsilon revealing that antibody binding does not induce any substantial rearrangements within CD3epsilon.
Title: T cell receptors are structures capable of initiating signaling in the absence of large conformational rearrangements.
CFTR dysfunction in T cells can lead directly to aberrant immune responses in CD3+ lymphocytes
Title: Lack of cystic fibrosis transmembrane conductance regulator in CD3+ lymphocytes leads to aberrant cytokine secretion and hyperinflammatory adaptive immune responses.
analysis of the transgenic integration site in immunodeficient tgepsilon26 human CD3epsilon transgenic mice
Title: Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice.
Polymorphisms of the CD3varepsilon ectodomain exist in mice,some of which lead to amino acid substitutions which cause structural changes and affect anti-CD3 antibody binding.
Title: Polymorphisms of the T cell receptor CD3delta and CD3epsilon chains affect anti-CD3 antibody binding and T cell activation.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (8) 1 citation
Chemically induced (ENU) (2)
Endonuclease-mediated (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Cd3e (e-PCR), detects polymorphism
- UniSTS:259590

AI504783 (e-PCR)
- UniSTS:179882

J02990 (e-PCR)
- UniSTS:179888

PMC113837P1 (e-PCR)
- UniSTS:270257

Homology

Homologs of the Cd3e gene: The Cd3e gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and frog.
Orthologs from Annotation Pipeline: 309 organisms have orthologs with human gene Cd3e
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane signaling receptor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of CD4-positive, alpha-beta T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of T cell activation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of T cell anergy	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within T cell costimulation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within T cell receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within adaptive immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in cell surface receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in cerebellum development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of interferon-gamma production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of interleukin-4 production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within lymphocyte activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative thymic T cell selection	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of CD4-positive, alpha-beta T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of T cell activation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of T cell anergy	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of T cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell-cell adhesion mediated by integrin	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cell-matrix adhesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of interferon-gamma production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of interleukin-2 production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of interleukin-4 production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive thymic T cell selection	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in response to nutrient	ISO Inferred from Sequence Orthology more info
acts_upstream_of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
part_of T cell receptor complex	ISO Inferred from Sequence Orthology more info
part_of alpha-beta T cell receptor complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
part_of alpha-beta T cell receptor complex	IDA Inferred from Direct Assay more info	PubMed
part_of alpha-beta T cell receptor complex	ISO Inferred from Sequence Orthology more info
located_in cell body	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in immunological synapse	IDA Inferred from Direct Assay more info	PubMed
located_in integral component of membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: T-cell surface glycoprotein CD3 epsilon chain

Names: T-cell surface antigen T3/Leu-4 epsilon chain

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007648.5 → NP_031674.1 T-cell surface glycoprotein CD3 epsilon chain precursor

See identical proteins and their annotated locations for NP_031674.1

Status: VALIDATED

Source sequence(s)

AC122305

Consensus CDS

CCDS23125.1

UniProtKB/Swiss-Prot

P22646

UniProtKB/TrEMBL

A6H6M1

Related

ENSMUSP00000099896.2, ENSMUST00000102832.3

Conserved Domains (2) summary

smart00077
Location:167 → 187

ITAM; Immunoreceptor tyrosine-based activation motif

pfam16681
Location:29 → 108

Ig_5; Ig-like domain on T-cell surface glycoprotein CD3 epsilon chain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

44910033..44920961 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006509966.4 → XP_006510029.1 T-cell surface glycoprotein CD3 epsilon chain isoform X1

See identical proteins and their annotated locations for XP_006510029.1

UniProtKB/Swiss-Prot

P22646

UniProtKB/TrEMBL

A6H6M1

Conserved Domains (2) summary

smart00077
Location:167 → 187

ITAM; Immunoreceptor tyrosine-based activation motif

pfam16681
Location:29 → 108

Ig_5; Ig-like domain on T-cell surface glycoprotein CD3 epsilon chain