Cd3e CD3 antigen, epsilon polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cd3eprovided by MGI
Official Full Name: CD3 antigen, epsilon polypeptideprovided by MGI
Primary source: MGI:MGI:88332
See related: Ensembl:ENSMUSG00000032093
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CD3; T3e; AI504783; CD3epsilon
Expression: Biased expression in thymus adult (RPKM 115.4), spleen adult (RPKM 18.7) and 1 other tissue See more
Orthologs: human all

Genomic context

Location:: 9 A5.2; 9 24.84 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
106	current	GRCm38.p4 (GCF_000001635.24)	9	NC_000075.6 (44998735..45009664, complement)
Build 37.2	previous assembly	MGSCv37 (GCF_000001635.18)	9	NC_000075.5 (44806826..44817673, complement)

Chromosome 9 - NC_000075.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

The data show that CD3epsilon chain couples to Cbl via simultaneous binding of both proteins to Numb, thus mediating TCR degradation
Title: CD3ε recruits Numb to promote TCR degradation.
IL-17-producing CD3(bright) gammadelta T cells responded promptly and strongly to pneumococcal infection and during skin inflammation.
Title: CD3bright signals on γδ T cells identify IL-17A-producing Vγ6Vδ1+ T cells.
these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling.
Title: LAPTM5 promotes lysosomal degradation of intracellular CD3ζ but not of cell surface CD3ζ.
these results indicate that membrane association of the CD3epsilon signaling domain is required for optimal thymocyte development and peripheral T cell function.
Title: Membrane association of the CD3ε signaling domain is required for optimal T cell development and function.
These results suggest that proteasome-mediated degradation is involved in hypophosphorylated LAT and PLCgamma1 in Dow2-induced anergic T cells. The novel CD3-specific Ab, Dow2, may provide us with a unique tool for inducing immunosuppression
Title: Novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLCγ1 following T-cell stimulation.
that Nck recruitment to the TCR is fundamental to mount an efficient T cell response in vivo, and that the Nck-CD3epsilon interaction may represent a target for pharmacological modulation of the immune response.
Title: Relevance of Nck-CD3 epsilon interaction for T cell activation in vivo.
Structure of murine CD3epsilon complexed with the mitogenic anti-CD3epsilon antibody 2C11 enabled structural comparisons of antibody-liganded and unliganded forms of CD3epsilon revealing that antibody binding does not induce any substantial rearrangements within CD3epsilon.
Title: T cell receptors are structures capable of initiating signaling in the absence of large conformational rearrangements.
CFTR dysfunction in T cells can lead directly to aberrant immune responses in CD3+ lymphocytes
Title: Lack of cystic fibrosis transmembrane conductance regulator in CD3+ lymphocytes leads to aberrant cytokine secretion and hyperinflammatory adaptive immune responses.
analysis of the transgenic integration site in immunodeficient tgepsilon26 human CD3epsilon transgenic mice
Title: Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice.
Polymorphisms of the CD3varepsilon ectodomain exist in mice,some of which lead to amino acid substitutions which cause structural changes and affect anti-CD3 antibody binding.
Title: Polymorphisms of the T cell receptor CD3delta and CD3epsilon chains affect anti-CD3 antibody binding and T cell activation.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation
Chemically induced (ENU) (2)

Pathways from BioSystems

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Adaptive Immune System, organism-specific biosystem (from REACTOME)
Adaptive Immune System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Chagas disease (American trypanosomiasis), organism-specific biosystem (from KEGG)
Chagas disease (American trypanosomiasis), organism-specific biosystemTrypanosoma cruzi is an intracellular protozoan parasite that causes Chagas disease. The parasite life cycle involves hematophagous reduviid bugs as vectors. Once parasites enter the host body, they ...
Chagas disease (American trypanosomiasis), conserved biosystem (from KEGG)
Chagas disease (American trypanosomiasis), conserved biosystemTrypanosoma cruzi is an intracellular protozoan parasite that causes Chagas disease. The parasite life cycle involves hematophagous reduviid bugs as vectors. Once parasites enter the host body, they ...
Costimulation by the CD28 family, organism-specific biosystem (from REACTOME)
Costimulation by the CD28 family, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Downstream TCR signaling, organism-specific biosystem (from REACTOME)
Downstream TCR signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Generation of second messenger molecules, organism-specific biosystem (from REACTOME)
Generation of second messenger molecules, organism-specific biosystemcomputationally inferred pathway (not manually curated)
HTLV-I infection, organism-specific biosystem (from KEGG)
HTLV-I infection, organism-specific biosystemHuman T-lymphotropic virus type 1 (HTLV-1) is a pathogenic retrovirus that is associated with adult T-cell leukemia/lymphoma (ATL). It is also strongly implicated in non-neoplastic chronic inflammato...
HTLV-I infection, conserved biosystem (from KEGG)
HTLV-I infection, conserved biosystemHuman T-lymphotropic virus type 1 (HTLV-1) is a pathogenic retrovirus that is associated with adult T-cell leukemia/lymphoma (ATL). It is also strongly implicated in non-neoplastic chronic inflammato...
Hematopoietic cell lineage, organism-specific biosystem (from KEGG)
Hematopoietic cell lineage, organism-specific biosystemBlood-cell development progresses from a hematopoietic stem cell (HSC), which can undergo either self-renewal or differentiation into a multilineage committed progenitor cell: a common lymphoid proge...
Hematopoietic cell lineage, conserved biosystem (from KEGG)
Hematopoietic cell lineage, conserved biosystemBlood-cell development progresses from a hematopoietic stem cell (HSC), which can undergo either self-renewal or differentiation into a multilineage committed progenitor cell: a common lymphoid proge...
Immune System, organism-specific biosystem (from REACTOME)
Immune System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem (from REACTOME)
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Measles, organism-specific biosystem (from KEGG)
Measles, organism-specific biosystemMeasles virus (MV) is highly contagious virus that leads infant death worldwide. Humans are the unique natural reservoir for this virus. It causes severe immunosuppression favouring secondary bacteri...
Measles, conserved biosystem (from KEGG)
Measles, conserved biosystemMeasles virus (MV) is highly contagious virus that leads infant death worldwide. Humans are the unique natural reservoir for this virus. It causes severe immunosuppression favouring secondary bacteri...
PD-1 signaling, organism-specific biosystem (from REACTOME)
PD-1 signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Phosphorylation of CD3 and TCR zeta chains, organism-specific biosystem (from REACTOME)
Phosphorylation of CD3 and TCR zeta chains, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Primary immunodeficiency, organism-specific biosystem (from KEGG)
Primary immunodeficiency, organism-specific biosystemPrimary immunodeficiencies (PIs) are a heterogeneous group of disorders, which affect cellular and humoral immunity or non-specific host defense mechanisms mediated by complement proteins, and cells ...
Primary immunodeficiency, conserved biosystem (from KEGG)
Primary immunodeficiency, conserved biosystemPrimary immunodeficiencies (PIs) are a heterogeneous group of disorders, which affect cellular and humoral immunity or non-specific host defense mechanisms mediated by complement proteins, and cells ...
T cell receptor signaling pathway, organism-specific biosystem (from KEGG)
T cell receptor signaling pathway, organism-specific biosystemActivation of T lymphocytes is a key event for an efficient response of the immune system. It requires the involvement of the T-cell receptor (TCR) as well as costimulatory molecules such as CD28. En...
T cell receptor signaling pathway, conserved biosystem (from KEGG)
T cell receptor signaling pathway, conserved biosystemActivation of T lymphocytes is a key event for an efficient response of the immune system. It requires the involvement of the T-cell receptor (TCR) as well as costimulatory molecules such as CD28. En...
TCR signaling, organism-specific biosystem (from REACTOME)
TCR signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Th1 and Th2 cell differentiation, organism-specific biosystem (from KEGG)
Th1 and Th2 cell differentiation, organism-specific biosystemImmunity to different classes of microorganisms is orchestrated by separate lineages of effector T helper (TH)-cells, which differentiate from naive CD4+ precursor cells in response to cues provided ...
Th1 and Th2 cell differentiation, conserved biosystem (from KEGG)
Th1 and Th2 cell differentiation, conserved biosystemImmunity to different classes of microorganisms is orchestrated by separate lineages of effector T helper (TH)-cells, which differentiate from naive CD4+ precursor cells in response to cues provided ...
Th17 cell differentiation, organism-specific biosystem (from KEGG)
Th17 cell differentiation, organism-specific biosystemInterleukin (IL)-17-producing helper T (Th17) cells serve as a subset of CD4+ T cells involved in epithelial cell- and neutrophil mediated immune responses against extracellular microbes and in the p...
Th17 cell differentiation, conserved biosystem (from KEGG)
Th17 cell differentiation, conserved biosystemInterleukin (IL)-17-producing helper T (Th17) cells serve as a subset of CD4+ T cells involved in epithelial cell- and neutrophil mediated immune responses against extracellular microbes and in the p...
Translocation of ZAP-70 to Immunological synapse, organism-specific biosystem (from REACTOME)
Translocation of ZAP-70 to Immunological synapse, organism-specific biosystemcomputationally inferred pathway (not manually curated)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystem (from WikiPathways)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystemXPodNet is a larger version of PodNet. Interactions from STRING database have been added to PodNet to build XPodNet. Hence, not all interactions found in XPodNet do necessarily have to be curated.

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Cd3e (e-PCR), detects polymorphism
- UniSTS:259590

AI504783 (e-PCR)
- UniSTS:179882

J02990 (e-PCR)
- UniSTS:179888

PMC113837P1 (e-PCR)
- UniSTS:270257

Homology

Homologs of the Cd3e gene: The Cd3e gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and frog.
Orthologs from Annotation Pipeline: 262 organisms have orthologs with human gene Cd3e
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
SH3 domain binding	ISO Inferred from Sequence Orthology more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
protein homodimerization activity	ISO Inferred from Sequence Orthology more info
transmembrane signaling receptor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Process	Evidence Code	Pubs
T cell costimulation	IGI Inferred from Genetic Interaction more info	PubMed
T cell differentiation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
T cell receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
adaptive immune response	IEA Inferred from Electronic Annotation more info
apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
cell surface receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info	PubMed
cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
cerebellum development	IMP Inferred from Mutant Phenotype more info	PubMed
dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed
immune system process	IEA Inferred from Electronic Annotation more info
lymphocyte activation	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
negative regulation of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
negative thymic T cell selection	IDA Inferred from Direct Assay more info	PubMed
positive regulation of T cell activation	IDA Inferred from Direct Assay more info	PubMed
positive regulation of T cell anergy	IDA Inferred from Direct Assay more info	PubMed
positive regulation of T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
positive regulation of T cell proliferation	ISO Inferred from Sequence Orthology more info
positive regulation of alpha-beta T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
positive regulation of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
positive regulation of cell-cell adhesion mediated by integrin	IGI Inferred from Genetic Interaction more info	PubMed
positive regulation of cell-matrix adhesion	IGI Inferred from Genetic Interaction more info	PubMed
positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
positive regulation of interferon-gamma production	IDA Inferred from Direct Assay more info	PubMed
positive regulation of interleukin-2 biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
positive regulation of interleukin-4 production	IDA Inferred from Direct Assay more info	PubMed
positive regulation of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
positive thymic T cell selection	IBA Inferred from Biological aspect of Ancestor more info	PubMed
protein homooligomerization	ISO Inferred from Sequence Orthology more info
response to nutrient	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
T cell receptor complex	ISO Inferred from Sequence Orthology more info
alpha-beta T cell receptor complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
alpha-beta T cell receptor complex	IDA Inferred from Direct Assay more info	PubMed
cell body	IDA Inferred from Direct Assay more info	PubMed
cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
dendritic spine	IDA Inferred from Direct Assay more info	PubMed
external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info	PubMed
external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
external side of plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
immunological synapse	IDA Inferred from Direct Assay more info	PubMed
integral component of membrane	IEA Inferred from Electronic Annotation more info
membrane	IEA Inferred from Electronic Annotation more info
plasma membrane	IDA Inferred from Direct Assay more info	PubMed
plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: T-cell surface glycoprotein CD3 epsilon chain

Names: T-cell surface antigen T3/Leu-4 epsilon chain

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007648.5 → NP_031674.1 T-cell surface glycoprotein CD3 epsilon chain precursor

See identical proteins and their annotated locations for NP_031674.1

Status: VALIDATED

Source sequence(s)

AC122305

Consensus CDS

CCDS23125.1

UniProtKB/Swiss-Prot

P22646

UniProtKB/TrEMBL

A6H6M1

Related

ENSMUSP00000099896.1, ENSMUST00000102832.2

Conserved Domains (3) summary

smart00077
Location:167 → 187

ITAM; Immunoreceptor tyrosine-based activation motif

smart00410
Location:35 → 83

IG_like; Immunoglobulin like

pfam16681
Location:29 → 103

Ig_5; Ig-like domain on T-cell surface glycoprotein CD3 epsilon chain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p4 C57BL/6J

Genomic

NC_000075.6 Reference GRCm38.p4 C57BL/6J

Range

44998735..45009664 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006509966.3 → XP_006510029.1 T-cell surface glycoprotein CD3 epsilon chain isoform X1

See identical proteins and their annotated locations for XP_006510029.1

UniProtKB/Swiss-Prot

P22646

UniProtKB/TrEMBL

A6H6M1

Conserved Domains (3) summary

smart00077
Location:167 → 187

ITAM; Immunoreceptor tyrosine-based activation motif

smart00410
Location:35 → 83

IG_like; Immunoglobulin like

pfam16681
Location:29 → 103

Ig_5; Ig-like domain on T-cell surface glycoprotein CD3 epsilon chain