1KHC: Crystal Structure Of The Pwwp Domain Of Mammalian Dna Methyltransferase Dnmt3b

The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids 223--357) has been structurally characterized at 1.8 A resolution. The N-terminal half of this domain resembles a barrel-like five-stranded structure, whereas the C-terminal half contains a five-helix bundle. The two halves are packed against each other to form a single structural module that exhibits a prominent positive electrostatic potential. The PWWP domain alone binds DNA in vitro, probably through its basic surface. We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b) and two N-terminal deletion mutants (Delta218 and Delta369) have approximately equal methyl transfer activity on unmethylated and hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.
PDB ID: 1KHCDownload
MMDB ID: 70915
PDB Deposition Date: 2001/11/29
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 1KHC: monomeric; determined by author
Molecular Components in 1KHC
Label Count Molecule
Protein (1 molecule)
DNA Cytosine-5 Methyltransferase 3B2(Gene symbol: Dnmt3b)
Molecule annotation
Chemicals (22 molecules)
Molecule information is not avaliable.
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Citing MMDB