1NGV,1R4M


Conserved Protein Domain Family
APPBP1_RUB

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cd01493: APPBP1_RUB 
Click on image for an interactive view with Cn3D
Ubiquitin activating enzyme (E1) subunit APPBP1. APPBP1 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to substrate proteins consisting of activating (E1), conjugating (E2), and/or ligating (E3) enzymes. E1 activates ubiquitin(-like) by C-terminal adenylation, and subsequently forms a highly reactive thioester bond between its catalytic cysteine and Ubls C-terminus. E1 also associates with E2 and promotes ubiquitin transfer to the E2's catalytic cysteine. Post-translational modification by Rub family of ubiquitin-like proteins (Ublps) activates SCF ubiquitin ligases and is involved in cell cycle control, signaling and embryogenesis. ABPP1 contains part of the adenylation domain.
Statistics
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PSSM-Id: 238770
Aligned: 15 rows
Threshold Bit Score: 523.406
Created: 10-Jan-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
dimer
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interaction [polypeptide binding site]
Evidence:
  • Structure:1NGV; human UBA3-APPBP1 complex

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1     ## ####### #                                 #  ##  #               ## ### #    
1NGV_A     11 QKYDRQLRLWGDHGQEALESAHVCLINATA------------TGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLqr 78  human
NP_648435  16 KKYDRQIRLWGEHGQTLLEAATVCLVNVTA------------VGCETAKGLVLPGIGGFTVADGSTVKEEDLGNNFFLds 83  fruit fly
XP_315544  35 RKYDRQIRLWGEHGQTVLENAQICLINATA------------LGTEILKGIVLPGIGGFTIVDHRPVTEEDVGCNFFLdl 102 Anopheles gambiae ...
AAO65877   14 TKYDRQLRIWGDQGQAALEKASICLLTCGP------------TGTEAMKNLVLGGVGSVTVVDGSKVEQSDMGNNFLLda 81  Japanese rice
P42744     22 TKYDRQLRIWGEVGQAALEEASICLLNCGP------------TGSEALKNLVLGGVGSITVVDGSKVQFGDLGNNFMVda 89  thale cress
EAK86779   26 QRYDRQLRLWASSGQSSLEKSRILVIGASA------------LSAQVLKNLVLPGIGSFVLLDDSIVDGADLGVNFFLqp 93  Ustilago maydis 521
EAA64147   17 RKYDRQLRLWAASGQQALEESRVLLVNSDGpwgnrstgvsgvVGVETLKNLVLPGVGGFTIVDPAVVTEPDLGVNFFLen 96  Aspergillus nidula...
NP_498037   8 TRYDRQVRLWGEEGQASIGSTSACVLGSDS------------LATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLha 75  nematode
EAK92126    7 AKYDRQLRLWASTGQSNLENSHICLINATS------------TGSEILKNLILPGIGQFTIIDEKKVTKQDLSSNFFLkn 74  Candida albicans S...
CAE85540   21 RKYDRQLRLWAASGQAALESANILLVNSGAg----------tVGVETLKNLILPGIGRFVIHDNALVDEADLGVNFFLdd 90  Neurospora crassa
Feature 1        #          #  ##                                                             
1NGV_A     79 -ssIGKNRAEAAMEFLQELNsdVSGSFVeespenlldndPSFFCRf--tVVVATqlp--esTSLRLADVLwns-----qi 148 human
NP_648435  84 -syLGKSKALACMQLLQELNpdVNGDYVdesadfllanrPNFFDSf--dLVIASnln--eqTLLLLAERLwel-----nv 153 fruit fly
XP_315544 103 -dsVGQPRAKRCMQLLQELNpdVNGDYVdehveqlidgqPDFFRSf--dVVVATsis--erTIMRLSNVLwdq-----ni 172 Anopheles gambiae ...
AAO65877   82 -ecLGQSRAKSVCSFLQELNdaVNAKFVeesplalidtnPSFFSQf--tVVIATqlp--erSLLKLDDICrka-----ni 151 Japanese rice
P42744     90 -ksVGQSKAKSVCAFLQELNdsVNAKFIeenpdtlittnPSFFSQf--tLVIATqlv--edSMLKLDRICrda-----nv 159 thale cress
EAK86779   94 gesEGKYAAEEMCRLLTEMNtsVASEAKlenpasllqsdPSFYAGf--tLVISVnqs--rsFDLALSDALwalqspspqv 169 Ustilago maydis 521
EAA64147   97 -esLGRSRAEETCRLLKELNpdVEGSFRtkpiaeilqeePGILAQhk-lILISGpie--hsSLRALCDGAkkl-----ai 167 Aspergillus nidula...
NP_498037  76 -ddIGRSRAEATLEKLTELNpsVSGSASsqpptalamedVEKLTTf--sVVVAAnqn--eeIDTTFAKVLyni-----rv 145 nematode
EAK92126   75 -qdLNEDLAVAIQKNLNELNndVNGHAIveslstilaqeSNLFWDqf-nVVIVSdy---tpNLEPLINLLwsk-----qi 144 Candida albicans S...
CAE85540   91 -scYGKPRAQCLASLLGELNpeVDGDWSpktk---ndslGSLLKQsplfTAIMYtypinhvELETLEQYSkeh-----kt 161 Neurospora crassa
Feature 1              #                                                                      
1NGV_A    149 PLLICRTYGLVGYMRIIIk-eHPVIESHPDn-ALEDLRLDKPFPELREHFQSydldhm--ekkdhSHTPWIVIIAKYLAQ 224 human
NP_648435 154 PLIYCRSLGMLGTIRLQIr-eHCIVEAHPDn-RQFDLRLEHPFDALREHLDGte---------vtSKVPWLLVLHKYLNV 222 fruit fly
XP_315544 173 PLIVARSVGFYGVARLQLr-eHCIVETHPDn-KQTDLRLEHPFEELKKHMAEaq---------itNKVPWLVVLYKVLQE 241 Anopheles gambiae ...
AAO65877  152 VLVAARSYGLTGLVRISVk-eHNVIESKPDh-FLDDLRLHNPWVELKQFAKSidindk--dpvvhKHTPYIVILVRLAEK 227 Japanese rice
P42744    160 KLVLVRSYGLAGFVRISVk-eHPIIDSKPDh-FLDDLRLNNPWPELKSFVETidlnvs-epaaahKHIPYVVILVKMAEE 236 thale cress
EAK86779  170 PLLRVRSAGMLAEMQISLk-eLGIIETHPD--SVVDLRITRPFPELLDAAQHfdlntn--dtmehSHIPFPIILIKKLAE 244 Ustilago maydis 521
EAA64147  168 PVLYTRSVGFYSTFSLQLpavFPIVETHPDpeSTQDLRLLNPWPELTAAGASirnlds-fddhqhGHVPYVLLLLHYLEK 246 Aspergillus nidula...
NP_498037 146 PFICIKTFGLIGTIRICIk-eHTIANSHEEn-PRPDLRLDAPFSKLIEMINEtnldem--tleqlRHTPYILLHFKALEV 221 nematode
EAK92126  145 PLFIVNTIGFYGSLNIIAn-eTTVIETHDPs-KLYDLRIDHPWPELQEFADSfdldkl--ndvehAHVPYIVIFIKALDH 220 Candida albicans S...
CAE85540  162 PLIAIHSTGFYSYFTIRLpgtFPIVDTHPDetATTDLRLLSPWPELVEFANTmtkdidsldnfehGHLPYVAILLHYLDK 241 Neurospora crassa
Feature 1                                                                                     
1NGV_A    225 WYSetn--griPKTYKEKEDFRDLIRQGilknengapeDEENFEEAIKNVNTALntt---------qiPSSIEDIFNDdr 293 human
NP_648435 223 WQKqqadgtqtPRNYKEKNQLKETIREEmk-------aDEENYEEAIKAVNTAFgag---------qvPKSLKAIFEDda 286 fruit fly
XP_315544 242 WVDahd--gqyPASYREKSEVRELIRSKmd-------gEQENFEEAIKAVNSSFggg---------kpSAAVREILQDdr 303 Anopheles gambiae ...
AAO65877  228 WADahd--grlPSTRQEKNEFKALIREYml------nlDEENYKEAVESSYKVSvtp---------giSDEIRQIIDDss 290 Japanese rice
P42744    237 WAQshs--gnlPSTREEKKEFKDLVKSKmv------stDEDNYKEAIEAAFKVFapr---------giSSEVQKLINDsc 299 thale cress
EAK86779  245 WQAahd--gqlPTTKDRDAFVKQVNASRvas-----npDAENFDEAISALGKHLwrpiasngvggggvPDEVEAMFQDaa 317 Ustilago maydis 521
EAA64147  247 WKEthn--gsvPSNYKEKTAFRDFVRSCartn--nsqgGEENYDEAVAAVLKSLnpf---------slRSSTREIFEMee 313 Aspergillus nidula...
NP_498037 222 FRKqrndpeafPSTTAERKELQAILMSFrrsseesgtkDSENFDEAKAAVIRAFqrt---------tiGSSVKSILSSpq 292 nematode
EAK92126  221 WRSqhd--gqvPITYADKKKFRLYVKSLsrn-----feLETNFIDADNAHLRPHqkt---------eiPESIIQLISIse 284 Candida albicans S...
CAE85540  242 WKAthn--gtyPSTYKEKTEFRQLVRDAartd--npegGEENFDEAAAAVLKTVapf---------slPSGLKEVFEYeh 308 Neurospora crassa
Feature 1                                                                                     
1NGV_A    294 cini-tkqtpsFWILARALKEFvakegqgnLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAkllqsigqap 372 human
NP_648435 287 ceql-nkksnvFWIMAKALKHFviheneghLPLPGVLPDMTANTDSYIALQHIYRQQALQDADQVYHKCQeylkqlalpa 365 fruit fly
XP_315544 304 cvnv-ntesnaFWIMARALKDFvdnegnglLPVPGVLPDMTADTNSYISLQTVYRNQAAHDAEIVFRRARqllkelnkpn 382 Anopheles gambiae ...
AAO65877  291 aevn--ssssdFWVLVAALKEFianegngeLPLEGTIPDMTSLTEYYVSLQKIYQAKAESDCLALEHHVKdilkridrdp 368 Japanese rice
P42744    300 aevn--snssaFWVMVAALKEFvlnegggeAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVIEERVKnilkkigrdp 377 thale cress
EAK86779  318 cdnl-tpastnFWILVRALREFvaaspsrcLPLSGSVPDMKATSSGYIKLQNIYRTKSLQDLAQFKKLVLdicmaag-ve 395 Ustilago maydis 521
EAA64147  314 crql-saasadFWVIASAVREFyet--hqlLPLPGSLPDMKAQSADYVSLQNIYKTKAREDIAEVTDIIRrlesqlg-ra 389 Aspergillus nidula...
NP_498037 293 cst----strpFWLICEALRRFvten-nnlLPLRGTLPDMTSDSSRYTRLATLFHEKALSDAQEVLRLTRevekerg-vg 366 nematode
EAK92126  285 tkklddsttsiFWIFIAALKNFlnln-dqlLPLPGTLPDMASDTENYVRLQQIYRDRALKDQKLFTEQVYqildslgrsr 363 Candida albicans S...
CAE85540  309 kdpi--qerstFWIIADAVKAFytk--hgsLPLPGNVPDMKAQSKVYVQLQNIYKAKARKDAAEVLQTARaiagt----g 380 Neurospora crassa
Feature 1                                                                                     
1NGV_A    373 esisEKELKLLCSNsafLRVVRCRSLAeeygldtinkdeiissmdnpdneivlylmlravdrfhkqqgrypgv------- 445 human
NP_648435 366 dsidERSVRLICKEaagLAVIRGTRIAeeyekssrllplvednelqgnltaynfalrayerflsecgnipg--------- 436 fruit fly
XP_315544 383 dlitDKDVRLFCREaanIAVVRGTKITdefdkgyhrsshiasvleqpnslmghyivlraldrfqadygclpg-------- 454 Anopheles gambiae ...
AAO65877  369 dsisRAYIKTFCKNarkLRVCRYRSMEeefsspvlsevqiyftdedycfamnfyvllravdrlaanynr----------- 437 Japanese rice
P42744    378 ssipKPTIKSFCKNarkLKLCRYRMVEdefrnpsvteiqkyladedysgamgfyillraadrfaanynkfpgq------- 450 thale cress
EAK86779  396 gkiaDDEIEAFVKHagyLKLIRGRSERqrcqepnqetamlafmdpvnpctfqhhialaaadqfleqngrypgvrqqafpa 475 Ustilago maydis 521
EAA64147  390 arvdDKDIEIFCKNashIQVIRGRDIPqvdggedtkkrlrlelnndeslipvylafevldtvvtgiqegkyhqd------ 463 Aspergillus nidula...
NP_498037 367 dvisDDVCYRFCKNadrIRVQYGDVLDyneetkaivekiresnideetrnqkvdeatwmllmravgrfqkekgrypgtn- 445 nematode
EAK92126  364 devnQESIASFCKNarlLFATVGSKDLindklvnqynynpttaissgggsgsssgssgggsitilddeekhklgiyfgll 443 Candida albicans S...
CAE85540  381 revdPAEVDLFCKNaafVKLINVEGGGdtaplgskerlqqvlrqemandqvaemtlqpmslfpiylalrglahcg----- 455 Neurospora crassa
Feature 1                                                                                     
1NGV_A    446 ----------------------------------------------------snyqveedigklkscltgflqeyglsvm 473 human
NP_648435 437 -----------------------------------------------------eciveqdigrlksiaakmlsdlgmhat 463 fruit fly
XP_315544 455 -----------------------------------------------------esdpesdttgmksiaakmlsdwgigtp 481 Anopheles gambiae ...
AAO65877  438 -------------------------------------------------------cpgiferlktaavsvmsemgmngap 462 Japanese rice
P42744    451 ---------------------------------------------------fdggmdedisrlkttalslltdlgcngsv 479 thale cress
EAK86779  476 llvgangtekdasgdvqpraakrqksetptgeldssitdvkmrditadqetskdqdelltiakklatkqfniaddgdewe 555 Ustilago maydis 521
EAA64147  464 --------------------------------------------------aldddgiwsseigrilaviaadaavdearn 493 Aspergillus nidula...
NP_498037 446 ---------------------------------------------gvpvsidaqdlkkrvevlirealkdeqdftsisnk 480 nematode
EAK92126  444 t-------------------------------------------fnlfidkyhykptindfnkflkiyhdeispkfklne 480 Candida albicans S...
CAE85540  456 -------------------------------------------------nassdktqiveavtsllsdnlsseeygdlne 486 Neurospora crassa
Feature 1                          #   #   #                     #       
1NGV_A    474 vkddyvhefcrygaaepHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATF 532 human
NP_648435 464 isddvlheicryggaelHAVSAFIGGCAAQEVIKIITKQYKPIDNTFIYNAITTESVTL 522 fruit fly
XP_315544 482 lsddlayeicryggaeiHSISAYLGGCIAHELIKLVTRQYRPFDNTFIYDGSTSQTETY 540 Anopheles gambiae str. PEST
AAO65877  463 lsedlitemcrfggaeiHPVAAFIGGVASQEVIKLVTKQFVPLLGTFIFNGIDHKSQVL 521 Japanese rice
P42744    480 lpddlihemcrfgaseiHVVSAFVGGIASQEVIKLVTKQFVPMLGTYIFNGIDHKSQLL 538 thale cress
EAK86779  556 kiensvgelvrsahaslPATTALMGGVVAQEAIKLITKQYVPADNTVIYDGIQQAIGVF 614 Ustilago maydis 521
EAA64147  494 rvldaaqelrrtkggelHNISSLTGGLVAQEALKVLTRQYVPLDNTCIFDGVRSSSAMY 552 Aspergillus nidulans FGSC A4
NP_498037 481 vtdtaiaeicrfgaaelHVISSYVGGIAAQEIIKLATNQYVPIDNTFIFDGHTQESATF 539 nematode
EAK92126  481 ngekifkeilthnttnyHNLNSLMGGIVSQEVLKSTTAQYIPLDNLFVFDGIHSKSEKF 539 Candida albicans SC5314
CAE85540  487 klgnvaedlaraeygelHNISALTGGMVAQEMIKIITKQYIPIDNTCVFDGIRSRTQVF 545 Neurospora crassa

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