3ANW,5GHR,5GHS


Conserved Protein Domain Family
GINS_B_archaea_Gins51

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cd21695: GINS_B_archaea_Gins51 
beta-strand (B) domain of archaeal GINS complex protein Gins51
The GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex is involved in both initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork. In archaeal DNA replication initiation, homo-hexameric MCM (mini-chromosome maintenance) unwinds the template double-stranded DNA to form the replication fork. MCM is activated by two proteins GINS and GAN (GINS-associated nuclease), which constitute the 'CMG' unwindosome complex together with the MCM core. While eukaryotic GINS complex is a tetrameric arrangement of four subunits Sld5, Psf1, Psf2 and Psf3, the archaeal complex consists of two different proteins, namely Gins51 and Gins23, and forms either an alpha2beta2-type heterotetramer composed of Gins51 and Gins23, or a Gins51-only alpha4-type homotetramer. The archaeal Gins51, as well as eukaryotic Sld5 and Psf1) have the alpha-helical (A) domain at the N-terminus and the beta-strand domain (B) at the C-terminus; this arrangement is called ABtype. Archaeal GINS contacts GAN by using the Gins51 B-domain as a hook, for the formation of the CMG helicase. The locations and contributions of the archaeal Gins subunit B domain to the tetramer formation, imply the possibility that the archaeal and eukaryotic GINS complexes contribute to DNA unwinding reactions by significantly different mechanisms in terms of the atomic details. This model represents the B-domain of Gins51.
Statistics
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PSSM-Id: 412031
Aligned: 42 rows
Threshold Bit Score: 58.7309
Created: 23-Apr-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heterodimer
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:heterodimer interface [polypeptide binding site]
Evidence:
  • Comment:archaeal GINS is either an alpha2beta2-type hetero-tetramer composed of Gins51 and Gins23, or a Gins51-only alpha4-type homo-tetramer; this Gins51 beta-strand domain contacts GAN using a partly common surface that is also used when it is attached to the GINS tetramer
  • Structure:5GHR: Thermococcus kodakarensis Gins51 C-terminal beta-strand domain (Gins51C) interacts with GAN (Gins-associated nuclease); contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #   #             #  #### #####                 ##
3ANW_A       134 VPKEAYIIQIDLPAVLGPdMKEYGPFMAGDMAIIPTVIGRALVErEAARRVR 185 Thermococcus kodakarensis
5GHR_B        26 VPKEAYIIQIDLPAVLGPdXKEYGPFXAGDXAIIPTVIGRALVErEAARRVR 77  Thermococcus kodakarensis KOD1
GBC69177     130 SRYEVVIFLKPSPKIVGVdMASYGPFREGDMAHIPAENAELLARrGVARRVQ 181 archaeon HR01
WP_004515156  83 HGYERVEFLTECPEFMGTdLEVYGPFDEGDTAEVPEDNAEILVNrGNAERTE 134 Haloarcula vallismortis
PIY59931     162 ITYVFVRFLTDVPRFLGEdLEVHGPFKEGDMASIPADIARVLIArDHAKEIE 213 Candidatus Woesearchaeota archaeon CG_4_10_...
OYT28529     138 REKIVVRILENLPAIVGTdMKVYGPFKKGDVVVLPRKNAETLIKkAKAKIIG 189 Thermofilum sp. ex4484_82
KRO29737     131 IKKVVIRFLSDVDEIIGAdLERYGPFNVEDIATIPYENAQALIAkKIATKVR 182 Nitrosopumilus sp. BACL13 MAG-121220-bin23
RLG46684      99 TGLSLVIFKTALPRFVGSdLEVYGPFRGGDLAILPREDAEVLEArGAVEVLE 150 Candidatus Korarchaeota archaeon
WP_011009095 107 QHYVVVQFLTSHPAIVTEdFVQIGPFSRGDLAKLPLRDAKDLEErGVARRFL 158 Pyrobaculum aerophilum
WP_048064936 177 EEYVVVRILKDLPTFTGA-DGRNYTVSAEDVVVLPQLNATGLIKrNAAKLIA 227 Methanosarcina acetivorans

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