2FB3,1TV7,2FB2


Conserved Protein Domain Family
Twitch_MoaA

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cd21117: Twitch_MoaA 
Iron-sulfur cluster-binding Twitch domain of GTP 3',8-cyclase
The iron-sulfur cluster-binding Twitch domain is found at the C-terminus of GTP 3',8-cyclase (EC 4.1.99.22), which is also called molybdenum cofactor biosynthesis protein A (MoaA) in bacteria and archaea, molybdenum cofactor biosynthesis protein 1 (MOCS1) in most eukaryotes, and molybdenum cofactor biosynthesis enzyme CNX2 in plants. GTP 3',8-cyclase is a radical S-adenosylmethionine (SAM) enzyme that catalyzes the first step in molybdopterin biosynthesis, the cyclization of guanosine triphosphate to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate, which is then converted to molybdopterin in subsequent steps. Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. GTP 3',8-cyclase contains an additional iron-sulfur cluster at the C-terminal Twitch domain that is involved in substrate binding. The Twitch domain may be related to another iron-sulfur cluster-binding domain found at the C-terminus of some radical SAM enzymes, the SPASM domain, named after the biochemically characterized members, AlbA, PqqE, anSMEs, and MftC, which are involved in Subtilosin A, Pyrroloquinoline quinone, Anaerobic Sulfatase, and Mycofactocin maturation, respectively.
Statistics
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PSSM-Id: 411052
Aligned: 196 rows
Threshold Bit Score: 64.4877
Created: 22-Jan-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: Fe-S cluster binding site [ion binding site], 3 residue positions
Conserved feature residue pattern:C C CClick to see conserved feature residue pattern help
Evidence:
  • Structure:2FB3: Staphylococcus aureus molybdenum cofactor biosynthesis protein A (MoaA) binds [4Fe-4S] cluster
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #  #             #                                                    
2FB3_A    256 VSQSFCSTCTRARLSSdGKFYGCLfaTVDGFNVKAFIrsgv----tdeELKEQFKALWQIRDDRYSDERTAQTVA 326 Staphylococcus aureus
A7Z9P0    260 VSDAFCASCNRARLSArGELFTCLf-ASSGYDVRKLVrggl----tddELTESIASVWRNRTDQYSIDRALSKTD 329 Bacillus velezensis FZB42
P9WJS2    268 TTEPFCATCDRSRLTAdGLWLHCLy-AISGINLREPLraga----thdDLVETVTTGWRRRTDRGAEQRLAQRER 337 Mycobacterium tuberculo...
Q3AVP9    264 ISAPFCGDCNRIRVTAdGQVFTCLf-ASQGVDLRPYLrtde----setQLRECLSELWTRRSDRFSEERSLQIDK 333 Synechococcus sp. CC9902
Q9EYN8    270 ITEPFCGDCNRLRVTAdGQAFTCLf-ASEGTDLRPFLhs-------eeTLEAVVRGLWQQRRDRYSEERQGFTAS 336 Synechococcus sp. WH 7803
Q3AGB7    270 ISEPFCGDCNRLRVTAdGQAFTCLf-SAEGTDLKPALas-------dlQLEQAMRQLWQRRQDRYSEERDPAAAA 336 Synechococcus sp. CC9605
Q8PBX1    261 VSVPFCGDCQRARVSAdGHLYTCLf-ASQGHDLKPALaeg------eqGLATHLRQRWSVRADRYSEVRASTSRR 328 Xanthomonas campestris ...
A0LVG0    257 ITQPFCRDCTRLRLSAvGEVFTCLf-AVRGHDLRSIVrsntdsaaidaALDEAITRIWSRRSDRYSELRALDSDG 330 Acidothermus cellulolyt...
Q7U3H2    269 ISEPFCGDCNRLRVTAdGQAFRCLf-ASVGTDLKPALhc-------eaELLRLVADLWRRRDDRYSDERQQTTGS 335 Synechococcus sp. WH 8102
O67929    248 VTKPFCRGCNRIRLSAdGKLYTCLf-SDKGHDLRNAV-----------DKENFIKEVWKDRKDRYSELRRQMKRE 310 Aquifex aeolicus VF5

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