2ZKM,1DJG,2FJU,1DJX,1QAS


Conserved Protein Domain Family
PI-PLCc_eukaryota

?
cd08558: PI-PLCc_eukaryota 
Click on image for an interactive view with Cn3D
Catalytic domain of eukaryotic phosphoinositide-specific phospholipase C and similar proteins
This family corresponds to the catalytic domain present in eukaryotic phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) and similar proteins. The higher eukaryotic PI-PLCs play a critical role in most signal transduction pathways, controlling numerous cellular events such as cell growth, proliferation, excitation and secretion. They strictly require Ca2+ for the catalytic activity. They display a clear preference towards the hydrolysis of the more highly phosphorylated membrane phospholipids PI-analogues, phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol-4-phosphate (PIP), to generate two important second messengers in eukaryotic signal transduction cascades, inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which then phosphorylates other molecules, leading to altered cellular activity. The eukaryotic PI-PLCs have a multidomain organization that consists of a PLC catalytic core domain, and various regulatory domains, such as the pleckstrin homology (PH) domain, EF-hand motif, and C2 domain. The catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a linker region. The catalytic mechanism of eukaryotic PI-PLCs is based on general base and acid catalysis utilizing two well conserved histidines and consists of two steps, a phosphotransfer and a phosphodiesterase reaction. The mammalian PI-PLCs consist of 13 isozymes, which are classified into six-subfamilies, PI-PLC-delta (1,3 and 4), -beta(1-4), -gamma(1,2), -epsilon, -zeta, and -eta (1,2). Ca2+ is required for the activation of all forms of mammalian PI-PLCs, and the concentration of calcium influences substrate specificity. This family also includes metazoan phospholipase C related but catalytically inactive proteins (PRIP), which belong to a group of novel inositol trisphosphate binding proteins. Due to the replacement of critical catalytic residues, PRIP does not have PLC enzymatic activity.
Statistics
?
PSSM-Id: 176501
View PSSM: cd08558
Aligned: 140 rows
Threshold Bit Score: 194.977
Threshold Setting Gi: 258597029
Created: 28-Sep-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Both prokaryotic and eukaryotic phosphatidylinositol-specific phospholipase Cs utilize a similar catalytic mechanism, a general base and acid catalysis involving two well conserved histidines. It consists of two steps, a phosphotransfer and a phosphodiesterase reaction.
  • Comment:Due to replacement of critical catalytic residues, metazoan Phospholipase C Related, but Catalytically Inactive Proteins (PRIP) do not have PLC enzymatic activity.
  • Structure:1DJX; Rat Phosphoinositide-specific phospholipase C-Delta1 catalytic residues.
    View structure with Cn3D
  • Structure:2FJU; Homo sapiens phospholipase C beta 2 catalytic site residues.
    View structure with Cn3D
  • Citation:PMID 9048554

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                        #                                                     #         
2ZKM_X       311 HHDMTQPLNHYFINSSHNTYLTAGQFSGLSSaEMYRQVLLSGCRCVELDCWKGkpp-------deePIITHGFTMTtdIF 383 human
XP_001766820 125 THDMTRPLSHYYIYASHNSYLTGNQLTSSSSiVPIIEALRNGCRVIELDCWEKqgqqcwsgstnndIVVLHGNTLTsaIP 204 Physcomitrella ...
BAD02928     147 TDDMNAPLSDYYIFSSHNTYLMGNQLCSPSStAPIVKALLDGCRVIELDCWNGsn---------krIDVLHGGTLTkaVP 217 Physcomitrella ...
ABR16641     106 HHDMTAPLSHYFIYTGHNSYLTGNQLSSDCSdAPIIKALNRGVRVIELDIWPNsak--------ddVLVLHGRTLTspVE 177 Sitka spruce
Q944C1       113 HQNMDAPLSHYFIFTGHNSYLTGNQLSSNCSeLPIADALRRGVRVVELDLWPRgt---------ddVCVKHGRTLTkeVK 183 thale cress
BAD02919     105 HQDMTQPLSHYFIFTGHNSYLTGNQLSSDSSdVPIAAALQRGVRVVELDLWPDdk---------ggIKVTHGNTLTspVA 175 Physcomitrella ...
Q9STZ3       104 HHDMHAPLSHYFIHTSLNSYFTGNVFGKYSI-LPIIEALEQGVRVVELDLWPDgr---------gsICVRPSWNFEkpLK 173 thale cress
CAL54863     116 ESEMTSEMSAYFVNSSHNTYLDGDQLFSRASvAAIAGALDRGCRVIELDVYDGkkrg-----dkprPIVTHGGTAVrpMY 190 Ostreococcus tauri
EEH59920     110 VSSMNEPLSHYFINSSHNTYLSGDQLFSRSLtFAIKRALLHGCRVIELDCYDGgr---------egPVVMHGITATqsIT 180 Micromonas pusi...
1DJX_B       163 YQDMDQPLSHYLVSSSHNTYLLEDQLTGPSStEAYIRALCKGCRCLELDCWDGpn---------qePIIYHGYTFTskIL 233 Norway rat
Feature 1                                                                                        
2ZKM_X       384 FKEAIEAIAESAFKTsPYPIILSFENHVDsprQQAKMAEYCRTIFGDMLLTEPLEkfplkpgvpLPSPEDLRGKILIKNK 463 human
XP_001766820 205 FKDCVIAIKENAFIAsQYPVIITIENHMGa-eMQKKAALVLREILGESLFYPSREqrp---plqFESPEVLKGFIIVSDQ 280 Physcomitrella ...
BAD02928     218 FEDCVKAIKDNAFTTsAYPVVVTIESHLDl-eHQKEAARVLNKILGNIMFVPPPNelp---pqsFRSPEQLKHRIIISDK 293 Physcomitrella ...
ABR16641     178 FIKCIRSIKEHAFVAsQYPVIITLEDHLTr-dLQAKAAETITKTFGELLYYPKSEvl-----eeFPSPESLKGRIIISTK 251 Sitka spruce
Q944C1       184 LGKCLESIKANAFAIsKYPVIITLEDHLTp-kLQFKVAKMITQTFGDMLYYHDSQgc-----qeFPSPEELKEKILISTK 257 thale cress
BAD02919     176 FEKCIKAIKANAFVSsKYPVVITLEDHLSs-pLQALAAETLTNILGEDLYYPPSSdgf----keLPSPESLKGKILISTK 250 Physcomitrella ...
Q9STZ3       174 LQECLDSIKEHAFTKcTYPLIITFKDGLKp-eLQSKATQMIQQTFNHMVYHHDPHsl-----evFPSPQQLRNKILISRR 247 thale cress
CAL54863     191 FEDAIKVIAERAHVAsEYPVIVTLENHASk-eTRAEMSRILRSTLGDKLWAPKSAvagn-qlkrWPSPLELKGRVIVRDK 268 Ostreococcus tauri
EEH59920     181 FRNALKTIKQDAHTTsEYPVIITMENHCSk-lKRVELAKILLEELGDKLFIPLSLhl-----nqWPSPSELKGRILIRDK 254 Micromonas pusi...
1DJX_B       234 FCDVLRAIRDYAFKAsPYPVILSLENHCSl-eQQRVMARHLRAILGPILLDQPLDgvt----tsLPSPEQLKGKILLKGK 308 Norway rat
Feature 1                                                                                        
2ZKM_X       464 Knqfsgptssskdtggeaegssppsapavwageegteleeeeveeeeeeesg---------------------------- 515 human
XP_001766820 281 Ppsdtiedqlavdpnaaqvlatdelddahastsdhtrhaqrlkkhikrahkkavlr------------------------ 336 Physcomitrella ...
BAD02928     294 Ppgdallsqaanepefaqqtllhevvrdpspdnrhehrhgrrgkkkklrrrqsqia------------------------ 349 Physcomitrella ...
ABR16641     252 Ppkeyletkttelqgdgdkeklktpdeepwgddipdygpdvpneresadpag---------------------------- 303 Sitka spruce
Q944C1       258 Ppkeyleandtkekdngekgkdsdedvwgkepedlistqsdldkvtssvnd----------------------------- 308 thale cress
BAD02919     251 Ppkeyleaavaqksalkdekilnelkkadklqeqstapvkspvekkiavppsektksiseekdlsekvgnlrvdsegesa 330 Physcomitrella ...
Q9STZ3       248 Ppkellyandddgkvgvrngvei--------------------------------------------------------- 270 thale cress
CAL54863     269 Ikhkqdelqrrrdqrrglcglcgtkkqskqrkvlpkslnvrkndkvcamqsii--------------------------- 321 Ostreococcus tauri
EEH59920     255 Igtkqvlreasgspylvrtqpipicrrfvdnaediarfeisddvtnreepceafs------------------------- 309 Micromonas pusi...
1DJX_B       309 Klggllpaggengseatdvsdeveaaemedeavrsqvqh----------------------------------------- 347 Norway rat
Feature 1                                                                                        
2ZKM_X       516 -------nldeeeikkmqsdegtaglevtayeemsslvnyiqptkfvsfefsaqknrsYVISSFTElKAYDLls--kaSV 586 human
XP_001766820 337 ---vantaakvlrksllvekievpetvefqeliylycakpsemkkakheegplvggdrAIMANLSE-PQLQHfia-rhPG 411 Physcomitrella ...
BAD02928     350 ---esriqklpsgklgdsgrpaiandpafdellyihcqkptemataqkkggplikgqhAIMANLSE-SQLDDlie-dhTD 424 Physcomitrella ...
ABR16641     304 -------pieedsgddegisqvnpdkdvapeykrlitiragkpkgvslkdsirvdgkqVKRVSLSE-PQLQKvar-shPT 374 Sitka spruce
Q944C1       309 ---------lnqddeergscesdtscqlqapeykrliaihagkpkgglrmalkvdpnkIRRLSLSE-QLLEKava-syGA 377 thale cress
BAD02919     331 dpapasspdgkkatltadsesddddnkknpeyarlitihqskpskgttvedrlkvegtVVRISLSE-TKLEKvte-efPE 408 Physcomitrella ...
Q9STZ3       271 ------------------------------------rqhpadpnyqslvsfhvveprgMLQNVLTG-KANKIqrpgwyET 313 thale cress
CAL54863     322 ------efprsstgvfdegavsssssdsegeqddedlkrivsvpnvkfrsfheardvpRFSCSWSE-RKLKLkiekesSK 394 Ostreococcus tauri
EEH59920     310 ----yfnfkfmrstnsyepptpssekvkqqvcnemfkslisienikikvikealeierVISCSWDE-LTLNNrkwaldKA 384 Micromonas pusi...
1DJX_B       348 --------------------kpkedklklvpelsdmiiycksvhfggfsspgtsgqafYEMASFSEsRALRLlq--esGN 405 Norway rat
Feature 1                                                                   
2ZKM_X       587 QFVDYNkRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTmDLPMQQNMAVFEFN 645 human
XP_001766820 412 SLIEYSkMNLGRVYPFGLRFNSSNADPMLAWSHGFQIAALNLQGrDRPVWLAKGFFQVN 470 Physcomitrella patens subsp. patens
BAD02928     425 SLIEFSkTNLTRVYPFGLRVTSSNADPMDAWEHGVQVAAINMQGrDRPVWISKAIFSRN 483 Physcomitrella patens
ABR16641     375 HVVRFThNNFLRIYPKGTRIDSSNYNPLLAWTHGAQMVAFNMQGyGRQLWLVHGFFRAN 433 Sitka spruce
Q944C1       378 DVIRFTqKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGyGRALWLMEGMFRAN 436 thale cress
BAD02919     409 LVVKFTqRNILRVYPAGNRVNSSNYDPTAAWIHGAQMVAQNMQGyGKELWQAHGKFRGN 467 Physcomitrella patens
Q9STZ3       314 DIISFTqKRFLRTRPQRKLLIYAPYKPQRAWMHGAQLIALSRKEeKEKLWLMQGMFRAN 372 thale cress
CAL54863     395 DIIEFTnAHLLRTYPAGHRIFSNNYDPSEAWDVGASFVALNFQAlDRYVWANRAKFRVN 453 Ostreococcus tauri
EEH59920     385 TMLDFTgRHLLRCYPNRRRVTSQNFDPSLAWSMGAQMVALNFQTnDLAMWLNRGKFVAN 443 Micromonas pusilla CCMP1545
1DJX_B       406 GFVRHNvSCLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTpGPEMDVYLGCFQDN 464 Norway rat

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap