1OCU,2CSK


Conserved Protein Domain Family
PX_SNX3_like

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cd06894: PX_SNX3_like 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of Sorting Nexin 3 and related proteins
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily is composed of SNX3, SNX12, and fungal Grd19. Grd19 is involved in the localization of late Golgi membrane proteins in yeast. SNX3/Grp19 associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer.
Statistics
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PSSM-Id: 132804
View PSSM: cd06894
Aligned: 12 rows
Threshold Bit Score: 171.102
Threshold Setting Gi: 40889192
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:1OCU_A; Saccharomyces cerevisiae SNX3 (Grd19p) PX domain binds Phosphatidylinositol-3-Phosphate; defined at 3.5A contacts.
    View structure with Cn3D
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                      # #  #                         ## 
1OCU_A        35 FLEIEVHNPKTHIpngmdskgMFTDYEIICRTNLPSFHKRVSKVRRRYSDFEFFRKXLIKeismlnhpKVMVPHLPGKIl 114 baker's yeast
2CSK_A        10 FLEIDIFNPQTVGvg----raRFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERd------sKIVVPPLPGKAl 79  human
NP_650214     32 FLEIDVVNPLTTMaag---kkRYTDYEVRMRTNLPVFKVKESSVRRRYSDFEWLRNELERd------sKIVVPPLPGKAw 102 fruit fly
AAY66964      32 YPEIDVGNPMTHGva----rkRYTDYEVRMRTNLPVFKNKESTVRRRYSDFEWLRSELERd------sKIVVPPLPGKAw 101 black-legged tick
XP_001630387  34 FLEIDVVNPETHGvg----kkRYTDYELRMRTNLPVFKQKESSVRRRYSDFEWLRSELERd------sKIVVPALPGKAl 103 starlet sea ane...
AAW26334      32 FLEIDVCRPLTHGdg----knRFTDYEVNLRTNLPIFAHKESSVRRRYSDFQWLRDELDRe------sKIVVPRLPSKAw 101 Schistosoma jap...
NP_492437     28 FLEIEVINPITHGvg----kmRYTDYEIRMRSNLPVFKQKESSVRRRYSDFEWVRAELERd------sKIVVPTLPGKSf 97  nematode
XP_780711     36 FLEIDVCNHQTHGig----nkRYTDYEVRMKTNLPVFKNKESTVRRRYSDFEFLKQELERd------sKIVVPALPGKAl 105 purple urchin
ABR16176      32 FLEIDVVNPVTTMgvg---kkRYTDYEVRMRTNLPVFKVKESSVRRRYSDFEWLRNELERd------sKIVVPPLPGKAl 102 Sitka spruce
XP_002116983  32 CLEIDVINPETHDlg----kkRFTDYEVKMKTNLPIFKKRETSVRRRYSDFEWLKSELERt------sKIVVPPLPGKAl 101 Trichoplax adha...
XP_002124567  30 FLEIEVSDPVTHGvgw---rnRFTDYAIKVQTNLPVFKHKESSVRRRYSDFEWLTSELERd------sKIVVPPLPGKAl 100 Ciona intestinalis
O60493        28 FLEIDVSNPQTVGvg----rgRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERe------sKVVVPPLPGKAf 97  human
Feature 1                    #        #                                 
1OCU_A       115 l---------snRFSNEVIEERRQGLNTWMQSVAGHPLLQsGSKVLVRFIEAEKF 160 baker's yeast
2CSK_A        80 krqlp-frgdegIFEESFIEERRQGLEQFINKIAGHPLAQ-NERCLHMFLQEEAI 132 human
NP_650214    103 krqmp-frgdegIFDESFIEERRKGLEAFINKIAGHPLAQ-NERCLHMFLQENVI 155 fruit fly
AAY66964     102 krqmp-frgdegIFDDEFIEERKKGLEVFINKVAGHPLAQ-NERCLHMFLQDQVI 154 black-legged tick
XP_001630387 104 krmlp-frgddgIFEDEFIEDRRQGLEAFINRVAGHPLAQ-NEKCLHMFLQEPMI 156 starlet sea anemone
AAW26334     102 krqlp-fradegIFDEDFIEERRKGLEEFINKVAGHPLAQ-NEKCLHMFLQEKTI 154 Schistosoma japonicum
NP_492437     98 krqlp-frsddgIFEEEFIENRRKALELFINKVAGHPLAQ-NERSLHIFLQEPTI 150 nematode
XP_780711    106 grqlpflkkgdgIFEDEFIEQRQTALEEFINKIAGHPLAQ-NEKSLHMFLQDKAL 159 purple urchin
ABR16176     103 krqlp-frgddgIFEEEFIEDRRKGLEVFINKIAGHPLAQ-NERCLHMFLQEPTI 155 Sitka spruce
XP_002116983 102 krqlp-frsddgIYEEGFIEERRSGLEGFINKIAGHPLAQ-NEKCLHMFLCDPIL 154 Trichoplax adhaerens
XP_002124567 101 kmqlp-frkdegLYDDAFIEERRKGLESFINKVAGHPLAQ-NERCLHMFLQEKDL 153 Ciona intestinalis
O60493        98 lrqlp-frgddgIFDDNFIEERKQGLEQFINKVAGHPLAQ-NERCLHMFLQDEII 150 human

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