Conserved Protein Domain Family
PX_SNX14

?
cd06877: PX_SNX14 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 14
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX14 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. It is expressed in the embryonic nervous system of mice, and is co-expressed in the motoneurons and the anterior pituary with Islet-1. SNX14 shows a similar domain architecture as SNX13, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.
Statistics
?
PSSM-Id: 132787
View PSSM: cd06877
Aligned: 13 rows
Threshold Bit Score: 152.145
Threshold Setting Gi: 110759141
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                         ###            
NP_001034378 554 SAWKISIPYVDFfddpsl----erkdreERIPVFCIDVErnd--------rraVGHEPEHWSVYRRYLEFYVLESKLTEF 621  chicken
EAW48633     458 AAWKISIPYVDFfedpss----erkekkERIPVFCIDVErnd--------rraVGHEPEHWSVYRRYLEFYVLESKLTEF 525  human
NP_001038258 555 SAWTISIPYVDFydde---------vkkERIPVFCIDVErnd--------rknVGHETESWSVYRKYVEFYVLESKLTEF 617  zebrafish
NP_001085023 564 SAWKISIPYVDFfedtnf----ekkdrkERIPVFCIDVErnd--------rkaVGHEPEHWSVYRKYIEFYVLESKLTEF 631  African clawed...
XP_966626    548 SSWRVSILSYQTn---------------NKVVSFCINVQrld---------vvEDVTKRHWIVLRKDQDFYTLKAKLVEF 603  red flour beetle
XP_001893532 589 SKWNVTIPCVEPrrdp---------vggHTMYVYVISVErfdlnddidqtslsATVAPQKWSLIRHYNEFYILESKLVEF 659  agent of lymph...
NP_496844    591 NCWKVNVAQVNGlrdk---------isdRVIFVFIIEVErp----------daKPHETQRWSIHRSFNEFYVLESKLNEF 651  nematode
XP_001952071 502 STWKVTDVTSAMkles--------gsngGQSLVFFITVEt------------vKNDLIKQWCVERRLADFYTLKSKLTEF 561  pea aphid
XP_002231298 509 SAWRVAIPRAESrstdvvl--gfryengKQYYVYIISVQrid--------ireGGKLFSHFPLYRRYNEFYVLENKLTEF 578  Florida lancelet
XP_002122266 556 STWRIVIPRVDVlrrddeisrgssastnEKMYAFCVEVKrvd--------iheDDDMPIQWQVLRRYNEFYVLENKLTEF 627  Ciona intestin...
XP_623699    528 TTWRISIPHIETg---------------DNQTLYVIAVH--------------NIAEGKSWTVLRRDQDFYVLRTRLAEF 578  honey bee
XP_781390    207 SAWRVSIPGVGMkme-----------ggKEGFVFIIDIQrvd--------vreGDGERNSWRVERMYHEFYALEQKLREF 267  purple urchin
EEC18620     514 SAWRVSIPDVEMrvd----------mqhRHYLAFIVEVQridv-------gdgDSPDDLHWTVARRYHEFYALESKLLEF 576  black-legged tick
Feature 1                     ##             #                              
NP_001034378 622 HGtf--pDAQLPSKRiigp-knyEFLKSKREEFQEYLQKLLQHpELSNSQLLADFLSPN 677  chicken
EAW48633     526 HGaf--pDAQLPSKRiigp-knyEFLKSKREEFQEYLQKLLQHpELSNSQLLADFLSPN 581  human
NP_001038258 618 HGpf--qDAQLPSKRiigp-knyEFLSSKRGEFEEYLQKLLHHpELSNSQLLADFLSPF 673  zebrafish
NP_001085023 632 HGtf--pDAQLPSKRiigp-knyEFLKSKREEFQEYLQNLLQHpELSNSQLLADFLSPN 687  African clawed frog
XP_966626    604 HGeneisDTPLPSRKa------gSSIEVRMKKYEEFLTKLLQKpLLRGSDLLFSFLTAE 656  red flour beetle
XP_001893532 660 HGnt-ikTESLPPRRffnc-xsrVYVESRREIFERFIQLLAKQrALKQSDLLFVFLSTE 716  agent of lymphatic filariasis
NP_496844    652 HGds-lrFSPLPTRKtfvt-rdkPFMEQHRLIFSTFLSTLSKQrILNRSELLLAFLSSN 708  nematode
XP_001952071 562 HGvf--vDARLPSRRi------lLQKTENNDIYMQFLNQLLQKpELRGSDLLHMFLTSI 612  pea aphid
XP_002231298 579 HGetlleDCCLPTKRiqltkpsqEFIDSKIAIFEKWLQVLLTKpALRGSELVHSFLSPG 637  Florida lancelet
XP_002122266 628 HGsl--aPASLPPKKfmr--rspEFLDSKKADFERFLKLLIGKpGLQHSELLYDFLLPE 682  Ciona intestinalis
XP_623699    579 HGdkelsDSPLPSRKnp-----hSSCAINRQRYEDFLEKLLSKpVLRSSELLYNFLTVP 632  honey bee
XP_781390    268 HGvf--eDAQLPVRRpfgs-ksqDFMEKKRPGLEKYLRVLLTKpILRGSQLLYTFLTTN 323  purple urchin
EEC18620     577 HGel--pVSPLPPRRsp----srQQQPPCRNALEKYLRDLLTVpRLRSSELLYSFLRHD 629  black-legged tick

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap