|
| Status |
Public on Dec 04, 2014 |
| Title |
F1 embryos of high sugar fed male flies - Replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w1118
developmental stage: Stage 17 embryos
tissue: Whole animal
treatment: 300g/L of sugar
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Purified total RNA, extracted with Trizol and treated with Ribo-ZeroTM rRNA removal kit from epicenter.
TruSeq® stranded sample preparation kit from Illumina.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
Purified total RNA was treated with Ribo-ZeroTM rRNA removal kit from epicenter.
|
| Data processing |
Reads were mapped using TopHat (Kim et al, 2013) v2.0.8 with -G option.
Gene expression values and significantly differentially expressed genes were calculated using Cuffdiff (Trapnell et al, 2012) v2.1.1 with upper quartile normalization and weighting multi-mapping reads (-N -u options).
Genome_build: BDGP5
Supplementary_files_format_and_content: Tab delimited text files. Gene expression values and statistical significance of differential expression calculated by CuffDiff.
|
| |
|
| Submission date |
Oct 23, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Tanya Vavouri |
| Organization name |
Josep Carreras Leukemia Research Institute
|
| Street address |
Ctra de Can Ruti, Cami de les Escoles, s/n
|
| City |
Badalona |
| State/province |
BARCELONA |
| ZIP/Postal code |
08019 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16479 |
| Series (1) |
| GSE62668 |
Paternal diet defines offspring chromatin state and intergenerational obesity. |
|
| Relations |
| Reanalyzed by |
GSM3287512 |
| BioSample |
SAMN03140390 |
| SRA |
SRX739981 |
