|
| Status |
Public on May 29, 2022 |
| Title |
Global Stable-isotope Tracing Metabolomics Reveals System-wide Metabolic Alternations in Aging Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Yet, delineating system-wide metabolic homeostasis at the whole-organism level remains non-trivial. Here, we develop a global isotope tracing metabolomics technology to measure labeled metabolites with a metabolome-wide coverage. Using Drosophila as an aging model organism, we probe the in vivo tracing kinetics with quantitative information on labeling patterns, extents and rates on a metabolome-wide scale. We curate a system-wide metabolic network to characterize metabolic homeostasis and disclose a system-wide loss of metabolic coordinations that impacts both intra- and inter-tissue metabolic homeostasis significantly during Drosophila aging. Importantly, we reveal an unappreciated metabolic diversion from glycolysis to serine metabolism and purine metabolism as Drosophila aging. The developed technology facilitates a system-level understanding of metabolic regulation in living organisms.
|
| |
|
| Overall design |
mRNA-seq of total RNAs from 3d and 30d flies, in triplicates, using Illumina Miseq.
|
| |
|
| Contributor(s) |
Wang R, Li J, Liu N, Zhu Z |
| Citation(s) |
35725845 |
| Submission date |
May 24, 2022 |
| Last update date |
Jun 30, 2022 |
| Contact name |
Ruohong Wang |
| E-mail(s) |
ruohongw3@gmail.com
|
| Phone |
15021841935
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
IRCBC
|
| Street address |
100 Haike Road, 13th building
|
| City |
Shanghai |
| State/province |
Not in US |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA842066 |
Less...
More...