RACGAP1 Rac GTPase activating protein 1 [Homo sapiens] - Gene

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Summary

Official Symbol: RACGAP1provided by HGNC
Official Full Name: Rac GTPase activating protein 1provided by HGNC
Primary source: HGNC:9804
See related: Ensembl:ENSG00000161800; HPRD:05402; MIM:604980; Vega:OTTHUMG00000169811
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CYK4; ID-GAP; HsCYK-4; MgcRacGAP
Summary: The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]

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Genomic context

Location :: 12q13.12

Sequence :: Chromosome: 12; NC_000012.11 (50382945..50419307, complement)

See RACGAP1 in Epigenomics, MapViewer

Chromosome 12 - NC_000012.11 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the level of RACGAP1 expression is sufficient to predict the early recurrence of HCC.
Title: Upregulation of Rac GTPase-activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma.
Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
study reports that mitotic complex genes Ect2, RacGAP, and MKLP1 are coordinately induced in S phase in proliferating T lymphocytes as well as in epithelial cells, depending upon activity of the CUX1 and E2F1 transcription factors
Title: CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase.
report here that (i) MgcRacGAP is phosphorylated by Aurora B and Cdk1, (ii) PP2A dephosphorylates Aurora B and Cdk1 phosphorylated sites and (iii) inhibition of PP2A abrogates MgcRacGAP/Ect2 interaction
Title: Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase.
Study demonstrates that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow.
Title: Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis.
These findings demonstrate that HIF-1alpha function is negatively affected by its interaction with MgcRacGAP.
Title: MgcRacGAP interacts with HIF-1alpha and regulates its transcriptional activity.
beta 2 chimerin has a role in rac-GAP-dependent inhibition of breast cancer cell proliferation
Title: Rac-GAP-dependent inhibition of breast cancer cell proliferation by {beta}2-chimerin.
MgcRacGAP controls the initiation of cytokinesis by regulating ECT2, which in turn induces the assembly of the contractile ring and triggers the ingression of the cleavage furrow
Title: MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis.
Cdk1 inactivation is sufficient to activate a signaling pathway leading to cytokinesis, which emanates from mitotic spindles and is regulated by ECT2, MgcRacGAP, and RhoA
Title: Inhibition of cyclin-dependent kinase 1 induces cytokinesis without chromosome segregation in an ECT2 and MgcRacGAP-dependent manner.

Submit: New GeneRIF Correction See all (20)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
Q9H0H5	Q96GD4	AURKB	HPRD	PubMed
Q9H0H5	P52757	CHN2	HPRD	PubMed
Q9H0H5	Q02241	KIF23	HPRD	PubMed
Q9H0H5	Q02779	MAP3K10	HPRD	PubMed
Q9H0H5	Q9Y6R4	MAP3K4	HPRD	PubMed
Q9H0H5	P01111	NRAS	HPRD	PubMed
Q9H0H5	O43663	PRC1	HPRD	PubMed
Q9H0H5	O95084	PRSS23	HPRD	PubMed
Q9H0H5	Q9H0H5	RACGAP1	HPRD	PubMed
Q9H0H5	P52198	RND2	HPRD	PubMed
Q9H0H5	SH3 multiple domains 2	SH3RF1	HPRD	PubMed
Q9H0H5	Solute carrier family 16, member 10	SLC16A10	HPRD	PubMed
Q9H0H5	Solute carrier family 26 member 8	SLC26A8	HPRD	PubMed
Q9H0H5	P68366	TUBA4A	HPRD	PubMed
Q9H0H5	P23258	TUBG1	HPRD	PubMed
Q9H0H5	P15498	VAV1	HPRD	PubMed
Q9H0H5	P31946	YWHAB	HPRD	PubMed
Q9H0H5	P61981	YWHAG	HPRD	PubMed
BioGRID:118892	BioGRID:114646	AURKB	BioGRID	PubMed	Biochemical Activity
BioGRID:118892	BioGRID:198584	Cd2ap	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:109142	GRB2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:214951	Kif23	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:116279	PRSS23	BioGRID	PubMed	Two-hybrid
BioGRID:118892	BioGRID:113810	RND2	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:118892	BioGRID:205072	Racgap1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:109072	SFN	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:125501	SLC26A8	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:118892	BioGRID:208480	Sh3rf1	BioGRID	PubMed	Two-hybrid
BioGRID:118892	BioGRID:203217	Shcbp1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:113252	VAV1	BioGRID	PubMed	Reconstituted Complex
BioGRID:118892	BioGRID:113361	YWHAB	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118892	BioGRID:113364	YWHAG	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
GTPase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
alpha-tubulin binding	IDA Inferred from Direct Assay more info	PubMed
beta-tubulin binding	IDA Inferred from Direct Assay more info	PubMed
gamma-tubulin binding	IDA Inferred from Direct Assay more info	PubMed
metal ion binding	IEA Inferred from Electronic Annotation more info
microtubule binding	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
blood coagulation	TAS Traceable Author Statement more info
cell cycle	IEA Inferred from Electronic Annotation more info
cell cycle cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
cell differentiation	IEA Inferred from Electronic Annotation more info
cytokinesis	IDA Inferred from Direct Assay more info	PubMed
cytokinesis, actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
cytokinesis, initiation of separation	IMP Inferred from Mutant Phenotype more info	PubMed
embryo development	ISS Inferred from Sequence or Structural Similarity more info
ion transport	IEA Inferred from Electronic Annotation more info
microtubule-based movement	TAS Traceable Author Statement more info
neuroblast proliferation	ISS Inferred from Sequence or Structural Similarity more info
positive regulation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
positive regulation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of cell cycle cytokinesis	IDA Inferred from Direct Assay more info	PubMed
positive regulation of cell cycle cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of attachment of spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of small GTPase mediated signal transduction	TAS Traceable Author Statement more info
small GTPase mediated signal transduction	TAS Traceable Author Statement more info
spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
spindle midzone assembly involved in mitosis	IDA Inferred from Direct Assay more info	PubMed
sulfate transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
acrosomal vesicle	IEA Inferred from Electronic Annotation more info
centralspindlin complex	IDA Inferred from Direct Assay more info	PubMed
cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IEA Inferred from Electronic Annotation more info
cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
cytoskeleton	IEA Inferred from Electronic Annotation more info
cytosol	TAS Traceable Author Statement more info
microtubule	IEA Inferred from Electronic Annotation more info
midbody	IDA Inferred from Direct Assay more info	PubMed
mitotic spindle	IDA Inferred from Direct Assay more info	PubMed
mitotic spindle	IMP Inferred from Mutant Phenotype more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
spindle midzone	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: rac GTPase-activating protein 1

Names: rac GTPase-activating protein 1; protein CYK4 homolg; male germ cell RacGap; GTPase activating protein

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001126103.1 → NP_001119575.1 rac GTPase-activating protein 1

Status: VALIDATED

Description

Transcript Variant: This variant (2) includes two alternate exons in the 5' UTR.

Source sequence(s)

AC025154, AK315785, AL136794, BC024144, DB076571

Consensus CDS

CCDS8795.1

UniProtKB/TrEMBL

B2RE34

UniProtKB/Swiss-Prot

Q9H0H5

Related

ENSP00000449370, OTTHUMP00000242163, ENST00000547905, OTTHUMT00000406001

Conserved Domains (4) summary

cd00029
Location:287 – 335
Blast Score: 117

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd04382
Location:346 – 539
Blast Score: 848

RhoGAP_MgcRacGAP; RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin ...

TIGR02168
Location:13 – 105
Blast Score: 94

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

smart00324
Location:360 – 533
Blast Score: 423

RhoGAP; GTPase-activator protein for Rho-like GTPases
NM_001126104.1 → NP_001119576.1 rac GTPase-activating protein 1

Status: VALIDATED

Description

Transcript Variant: This variant (3) omits two alternate exons in the 5' UTR.

Source sequence(s)

AC025154, AK000733, BC032754, DA441098, DB076571

Consensus CDS

CCDS8795.1

UniProtKB/Swiss-Prot

Q9H0H5

Related

ENSP00000404808, OTTHUMP00000242161, ENST00000454520, OTTHUMT00000405999

Conserved Domains (4) summary

cd00029
Location:287 – 335
Blast Score: 117

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd04382
Location:346 – 539
Blast Score: 848

RhoGAP_MgcRacGAP; RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin ...

TIGR02168
Location:13 – 105
Blast Score: 94

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

smart00324
Location:360 – 533
Blast Score: 423

RhoGAP; GTPase-activator protein for Rho-like GTPases
NM_013277.3 → NP_037409.2 rac GTPase-activating protein 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) includes two alternate exons in the 5' UTR.

Source sequence(s)

AC025154, AL136794, DB076571

Consensus CDS

CCDS8795.1

UniProtKB/Swiss-Prot

Q9H0H5

Related

ENSP00000404190, OTTHUMP00000242159, ENST00000427314, OTTHUMT00000405997

Conserved Domains (4) summary

cd00029
Location:287 – 335
Blast Score: 117

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd04382
Location:346 – 539
Blast Score: 848

RhoGAP_MgcRacGAP; RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin ...

TIGR02168
Location:13 – 105
Blast Score: 94

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

smart00324
Location:360 – 533
Blast Score: 423

RhoGAP; GTPase-activator protein for Rho-like GTPases

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000012.11 Reference GRCh37.p5 Primary Assembly

Range

50382945..50419307, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000144.1 Alternate HuRef

Range

47414446..47451548, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC025154.31 (41550..77826)	None
genomic	CH471111.1	EAW58113.1
		EAW58114.1
		EAW58115.1
mRNA	AB030251.1	BAA90247.1
mRNA	AB040911.1	BAA96002.1
mRNA	AK000733.1	BAA91347.1
mRNA	AK022726.1	BAB14206.1
mRNA	AK225901.1	None
mRNA	AK315785.1	BAG38131.1
mRNA	AL136794.1	CAB66728.1
mRNA	BC024144.2	AAH24144.1
mRNA	BC032754.1	AAH32754.1
mRNA	CR533565.1	CAG38596.1
mRNA	DA441098.1	None
mRNA	DB076571.1	None
other-genetic	AM393855.1	CAL38730.1
other-genetic	DQ894409.2	ABM85335.1
other-genetic	EU176264.1	ABW03715.1