Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF706
Cytogenetic location: 8q22.3 Genomic coordinates (GRCh38): 8:101,197,052-101,206,222 (from NCBI)
ZNF706 is a zinc finger protein that is involved in regulation of cell volume (Dossena et al., 2011; Tamma et al., 2011).
By RT-PCR, Colombo et al. (2013) cloned human ZNF706 from a larynx tissue sample. ZNF706 encodes a 76-amino acid protein with a calculated molecular mass of 8.49 kD.
Dossena et al. (2011) stated that the ZNF706 gene maps to chromosome 8q22.3.
Using purified recombinant protein expressed in E. coli, Colombo et al. (2013) showed that human ZNF706 protein was composed of 35% alpha helices, 10% beta strands, 27% turns, and 28% random coils. The 3-dimensional structure generated by molecular modeling was in agreement with the predicted secondary structure.
Dossena et al. (2011) found that overexpression of human ZNF706, which they called HSPC038, or the chloride channel ICLN (602158) increased the swelling-induced chloride current (ICl-swell) in HEK293 Phoenix cells. In contrast, knockdown of HSPC038 impaired ICl-swell in HEK293 Phoenix cells. Analysis with purified recombinant proteins revealed that ICLN and HSPC038 interacted directly. Fluorescence resonance energy transfer (FRET) experiments in principal collecting duct M1 cells showed that hypotonic shock induced HSPC038 translocation toward the cell membrane in spatial proximity to ICLN and significantly increased interaction between the proteins. With increased interaction with ICLN, HSPC038 improved translocation of ICLN to the cell membrane but did not modify expression of endogenous ICLN. Drug-induced interaction of HSPC038 with ICLN also upregulated ICl-swell. By nuclear magnetic resonance (NMR) analysis, the authors identified putative interacting sites for the ICLN/HSPC038 complex.
Using FRET analysis, Tamma et al. (2011) showed that treatment with epidermal growth factor (EGF; 131530) caused direct interaction between ICLN and HSPC038 and significantly enhanced their direct interaction with the plasma membrane in transfected NIH-3T3 mouse cells. Electrophysiologic studies revealed that EGF stimulation led to activation of a chloride current of modest magnitude resembling ICl-swell in NIH-3T3 cells in isotonic conditions. In contrast, EGF markedly upregulated ICl-swell in both naive and ICLN-overexpressing NIH-3T3 cells. The results indicated that EGF likely exerted its role in the modulation of volume-sensitive chloride currents through redistribution of ICLN and HSPC038 to the plasma membrane.
Colombo, J., Provazzi, P. J. S., Calmon, M. F., Pires, L. C., Rodrigues, N. C., Peti, P., Jr., Fossey, M. A., de Sousa, F. P., Canduri, F., Rahal, P. Expression, purification and molecular analysis of the human ZNF706 protein. Biol. Proced. Online 15: 10, 2013. [PubMed: 24060497] [Full Text: https://doi.org/10.1186/1480-9222-15-10]
Dossena, S., Gandini, R., Tamma, G., Vezzoli, V., Nofziger, C., Tamplenizza, M., Salvioni, E., Bernardinelli, E., Meyer, G., Valenti, G., Wolf-Watz, M., Furst, J., Paulmichl, M. The molecular and functional interaction between ICln and HSPC038 proteins modulates the regulation of cell volume. J. Biol. Chem. 286: 40659-40670, 2011. [PubMed: 21917931] [Full Text: https://doi.org/10.1074/jbc.M111.260430]
Tamma, G., Dossena, S., Nofziger, C., Valenti, G., Svelto, M., Paulmichl, M. EGF stimulates ICl-swell by a redistribution of proteins involved in cell volume regulation. Cell. Physiol. Biochem. 28: 1191-1202, 2011. [PubMed: 22179007] [Full Text: https://doi.org/10.1159/000335851]