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Conserved domains on  [gi|407968025|dbj|BAM61263|]
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UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase [Streptococcus dysgalactiae subsp. equisimilis RE378]

Protein Classification

Mur ligase family protein( domain architecture ID 11433679)

Mur ligase family protein such as MurC, MurD, and MurE, which catalyze consecutive steps in the synthesis of peptidoglycan

CATH:  3.90.190.20|3.40.1190.10|3.40.1390.10
EC:  6.3.2.13
Gene Ontology:  GO:0005524|GO:0000287|GO:0009252|GO:0008765
PubMed:  11124264|28201974
SCOP:  4000234

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MurT_C pfam08353
MurT ligase C-terminal; This entry represents the C-terminal domain of MurT ligase from ...
 319-429 5.93e-44

MurT ligase C-terminal; This entry represents the C-terminal domain of MurT ligase from Gram-positive bacteria. MurT is part of the bi-enzymatic complex MurT-GatD involved in the amidation of the alpha-carboxyl group of the D-isoglutamate residue in Lipid II in the peptidoglycan layer to produce D-isoglutamine. This domain contains an aspartate at position 349, the third residue in the catalytic triad GatD-C94, GatD-H189, MurT-D349. This C-terminal domain is built around a central six-stranded, predominantly parallel beta-sheet that is sandwiched between four alpha-helices on one side and two alpha-helices on the other. The MurT-GatD heterodimer adopts a boomerang-shaped conformation, with GatD packing against the C-terminal domain of MurT.


:

Pssm-ID: 462442  Cd Length: 110  Bit Score: 149.56  E-value: 5.93e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  319 LIKNPVGASQALEMIQLADYPFSLSVLLNANYADGIDTSWIWDANFELITHMPITEINAGGVRHSEIARRLRVTGFDDTK 398
Cdd:pfam08353   1 LVKNPVGFNQVLNYIASDPGPKSLLLLLNDNYADGRDVSWIWDVDFEKLNDSNIKKIIVSGDRAYDMALRLKYAGVPEEK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 407968025  399 IKQAEKLEQITEAIeKQESKHAYILATYTAM 429
Cdd:pfam08353  81 IEIEEDLEEALDAI-KAPTENVYILPTYTAM 110
MurC super family cl34041
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 39-294 3.01e-15

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


The actual alignment was detected with superfamily member COG0773:

Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 77.41  E-value: 3.01e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  39 DILDSLSKDYEIVVITGTNGKTLTTALTVGILKEAfgdvltnpsGAN---MITGITAAF-LSAKKGKSgkHIAVLEIDE- 113
Cdd:COG0773   95 EMLAELMRGKRSIAVAGTHGKTTTTSMLAHILEEA---------GLDptfLIGGILNNFgTNARLGDG--DYFVAEADEs 163

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 114 -ASLpkitNYLKPSLFVYTNIFRDQMDrygeIYTTYQMIVDG----ARNAPKA-TILANGDSPIfsSKDIV----NPVQY 183
Cdd:COG0773  164 dGSF----LHYSPDIAVVTNIEADHLD----IYGDLEAIKEAfhefARNVPFYgLLVLCADDPG--LRELLprcgRPVIT 233

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 184 YGFDtakhapqlahyntegilcpkcehilqyrlntyanlgdficlncqfqrPTLDYQLTELTAITHQSS-EFVIDGQNY- 261
Cdd:COG0773  234 YGFS-----------------------------------------------EDADYRAENIRIDGGGSTfDVLRRGEELg 266

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 407968025 262 --KINVGGLYNIYNALAAVSVAEFFGVSPEKIKAG 294
Cdd:COG0773  267 evELNLPGRHNVLNALAAIAVALELGVDPEAIAEA 301
 
Name Accession Description Interval E-value
MurT_C pfam08353
MurT ligase C-terminal; This entry represents the C-terminal domain of MurT ligase from ...
 319-429 5.93e-44

MurT ligase C-terminal; This entry represents the C-terminal domain of MurT ligase from Gram-positive bacteria. MurT is part of the bi-enzymatic complex MurT-GatD involved in the amidation of the alpha-carboxyl group of the D-isoglutamate residue in Lipid II in the peptidoglycan layer to produce D-isoglutamine. This domain contains an aspartate at position 349, the third residue in the catalytic triad GatD-C94, GatD-H189, MurT-D349. This C-terminal domain is built around a central six-stranded, predominantly parallel beta-sheet that is sandwiched between four alpha-helices on one side and two alpha-helices on the other. The MurT-GatD heterodimer adopts a boomerang-shaped conformation, with GatD packing against the C-terminal domain of MurT.


Pssm-ID: 462442  Cd Length: 110  Bit Score: 149.56  E-value: 5.93e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  319 LIKNPVGASQALEMIQLADYPFSLSVLLNANYADGIDTSWIWDANFELITHMPITEINAGGVRHSEIARRLRVTGFDDTK 398
Cdd:pfam08353   1 LVKNPVGFNQVLNYIASDPGPKSLLLLLNDNYADGRDVSWIWDVDFEKLNDSNIKKIIVSGDRAYDMALRLKYAGVPEEK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 407968025  399 IKQAEKLEQITEAIeKQESKHAYILATYTAM 429
Cdd:pfam08353  81 IEIEEDLEEALDAI-KAPTENVYILPTYTAM 110
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 39-294 3.01e-15

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 77.41  E-value: 3.01e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  39 DILDSLSKDYEIVVITGTNGKTLTTALTVGILKEAfgdvltnpsGAN---MITGITAAF-LSAKKGKSgkHIAVLEIDE- 113
Cdd:COG0773   95 EMLAELMRGKRSIAVAGTHGKTTTTSMLAHILEEA---------GLDptfLIGGILNNFgTNARLGDG--DYFVAEADEs 163

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 114 -ASLpkitNYLKPSLFVYTNIFRDQMDrygeIYTTYQMIVDG----ARNAPKA-TILANGDSPIfsSKDIV----NPVQY 183
Cdd:COG0773  164 dGSF----LHYSPDIAVVTNIEADHLD----IYGDLEAIKEAfhefARNVPFYgLLVLCADDPG--LRELLprcgRPVIT 233

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 184 YGFDtakhapqlahyntegilcpkcehilqyrlntyanlgdficlncqfqrPTLDYQLTELTAITHQSS-EFVIDGQNY- 261
Cdd:COG0773  234 YGFS-----------------------------------------------EDADYRAENIRIDGGGSTfDVLRRGEELg 266

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 407968025 262 --KINVGGLYNIYNALAAVSVAEFFGVSPEKIKAG 294
Cdd:COG0773  267 evELNLPGRHNVLNALAAIAVALELGVDPEAIAEA 301
murD TIGR01087
UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation ...
 17-309 2.52e-14

UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273436 [Multi-domain]  Cd Length: 433  Bit Score: 74.68  E-value: 2.52e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   17 LILKKLGRGSTYPGRLALAFDKDildslskDYEIVVITGTNGKTLTTALTVGILKEAFGDVltnPSGANmitgITAAFLS 96
Cdd:TIGR01087  78 LVQAAAKRGIPVVGDIELFLRLV-------PLPVVAITGTNGKTTTTSLLYHLLKAAGLKA---FLGGN----IGTPALE 143

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   97 AKKGKsGKHIAVLEIDEASLpKITNYLKPSLFVYTNIFRDQMDRYG--EIYT-TYQMIVDGARNApkATILANGDSPIFS 173
Cdd:TIGR01087 144 VLDQE-GAELYVLELSSFQL-ETTESLRPEIALILNISEDHLDWHGsfEDYVaAKLKIFARQTEG--DVAVLNADDPRFA 219

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  174 SKDIVNPVQYYGFDTakhapqlahyntegilcpkcEHILQYRLNTYanlgdficlncqfqrptldyqlteltaithqSSE 253
Cdd:TIGR01087 220 RLAQKSKAQVIWFSV--------------------EKDAERGLCIR-------------------------------DGG 248

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 407968025  254 FVIDGQNYKINVGGLYNIYNALAAVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFK 309
Cdd:TIGR01087 249 LYLKPNDLEGSLLGLHNAENILAAIALAKSLGLNLEAILEALRSFKGLPHRLEYVG 304
murD PRK14106
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional
 50-306 2.81e-09

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional


Pssm-ID: 184511 [Multi-domain]  Cd Length: 450  Bit Score: 58.83  E-value: 2.81e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  50 IVVITGTNGKTLTTALTVGILKEAFGDVLTnpsGANmitgITAAFLSAKKGKSGKHIAVLEIDEASLPKItNYLKPSLFV 129
Cdd:PRK14106 110 IVAITGTNGKTTTTTLLGEIFKNAGRKTLV---AGN----IGYPLIDAVEEYGEDDIIVAEVSSFQLETI-KEFKPKVGC 181

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 130 YTNIFRDQMDRYGEIyttyqmivDGARNApKATILANGDSPIFSSKDIVNPVqyygfdTAKHAPQLahyntegilcpKCE 209
Cdd:PRK14106 182 ILNITPDHLDRHKTM--------ENYIKA-KARIFENQRPSDYTVLNYDDPR------TRSLAKKA-----------KAR 235

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 210 HILQYRLNTYANlGDFIclncqfqrptldyQLTELTAITHQSSEFVIDGQNYKINvgGLYNIYNALAAVSVAEFFGVSPE 289
Cdd:PRK14106 236 VIFFSRKSLLEE-GVFV-------------KNGKIVISLGGKEEEVIDIDEIFIP--GEHNLENALAATAAAYLLGISPD 299

                        250
                 ....*....|....*..
gi 407968025 290 KIKAGFNKSKAVFGRQE 306
Cdd:PRK14106 300 VIANTLKTFKGVEHRIE 316
Mur_ligase_M pfam08245
Mur ligase middle domain;
53-281 1.88e-08

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 54.23  E-value: 1.88e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   53 ITGTNGKTLTTALTVGILKEAFGDVLT-----NPSGANMITGITAAFLSAKKGKSGKHIAVLEIDEASLPK--ITNYLKP 125
Cdd:pfam08245   1 VTGTNGKTTTTELIAAILSLAGGVIGTigtyiGKSGNTTNNAIGLPLTLAEMVEAGAEYAVLEVSSHGLGEgrLSGLLKP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  126 SLFVYTNIFRDQMDRYG---EIYTTYQMIVDGARNAPKATIlaNGDSPIFS-----SKDIVNPVQYYGFDTAKhapqlah 197
Cdd:pfam08245  81 DIAVFTNISPDHLDFHGtmeNYAKAKAELFEGLPEDGIAVI--NADDPYGAfliakLKKAGVRVITYGIEGEA------- 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  198 yntegilcpkcehilqyrlntyanlgdficlncqfqrptlDYQLTELTAiTHQSSEFVI---DGQNYKINVG--GLYNIY 272
Cdd:pfam08245 152 ----------------------------------------DLRAANIEL-SSDGTSFDLftvPGGELEIEIPllGRHNVY 190


                  ....*....
gi 407968025  273 NALAAVSVA 281
Cdd:pfam08245 191 NALAAIAAA 199
 
Name Accession Description Interval E-value
MurT_C pfam08353
MurT ligase C-terminal; This entry represents the C-terminal domain of MurT ligase from ...
 319-429 5.93e-44

MurT ligase C-terminal; This entry represents the C-terminal domain of MurT ligase from Gram-positive bacteria. MurT is part of the bi-enzymatic complex MurT-GatD involved in the amidation of the alpha-carboxyl group of the D-isoglutamate residue in Lipid II in the peptidoglycan layer to produce D-isoglutamine. This domain contains an aspartate at position 349, the third residue in the catalytic triad GatD-C94, GatD-H189, MurT-D349. This C-terminal domain is built around a central six-stranded, predominantly parallel beta-sheet that is sandwiched between four alpha-helices on one side and two alpha-helices on the other. The MurT-GatD heterodimer adopts a boomerang-shaped conformation, with GatD packing against the C-terminal domain of MurT.


Pssm-ID: 462442  Cd Length: 110  Bit Score: 149.56  E-value: 5.93e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  319 LIKNPVGASQALEMIQLADYPFSLSVLLNANYADGIDTSWIWDANFELITHMPITEINAGGVRHSEIARRLRVTGFDDTK 398
Cdd:pfam08353   1 LVKNPVGFNQVLNYIASDPGPKSLLLLLNDNYADGRDVSWIWDVDFEKLNDSNIKKIIVSGDRAYDMALRLKYAGVPEEK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 407968025  399 IKQAEKLEQITEAIeKQESKHAYILATYTAM 429
Cdd:pfam08353  81 IEIEEDLEEALDAI-KAPTENVYILPTYTAM 110
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 39-294 3.01e-15

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 77.41  E-value: 3.01e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  39 DILDSLSKDYEIVVITGTNGKTLTTALTVGILKEAfgdvltnpsGAN---MITGITAAF-LSAKKGKSgkHIAVLEIDE- 113
Cdd:COG0773   95 EMLAELMRGKRSIAVAGTHGKTTTTSMLAHILEEA---------GLDptfLIGGILNNFgTNARLGDG--DYFVAEADEs 163

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 114 -ASLpkitNYLKPSLFVYTNIFRDQMDrygeIYTTYQMIVDG----ARNAPKA-TILANGDSPIfsSKDIV----NPVQY 183
Cdd:COG0773  164 dGSF----LHYSPDIAVVTNIEADHLD----IYGDLEAIKEAfhefARNVPFYgLLVLCADDPG--LRELLprcgRPVIT 233

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 184 YGFDtakhapqlahyntegilcpkcehilqyrlntyanlgdficlncqfqrPTLDYQLTELTAITHQSS-EFVIDGQNY- 261
Cdd:COG0773  234 YGFS-----------------------------------------------EDADYRAENIRIDGGGSTfDVLRRGEELg 266

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 407968025 262 --KINVGGLYNIYNALAAVSVAEFFGVSPEKIKAG 294
Cdd:COG0773  267 evELNLPGRHNVLNALAAIAVALELGVDPEAIAEA 301
murD TIGR01087
UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation ...
 17-309 2.52e-14

UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273436 [Multi-domain]  Cd Length: 433  Bit Score: 74.68  E-value: 2.52e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   17 LILKKLGRGSTYPGRLALAFDKDildslskDYEIVVITGTNGKTLTTALTVGILKEAFGDVltnPSGANmitgITAAFLS 96
Cdd:TIGR01087  78 LVQAAAKRGIPVVGDIELFLRLV-------PLPVVAITGTNGKTTTTSLLYHLLKAAGLKA---FLGGN----IGTPALE 143

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   97 AKKGKsGKHIAVLEIDEASLpKITNYLKPSLFVYTNIFRDQMDRYG--EIYT-TYQMIVDGARNApkATILANGDSPIFS 173
Cdd:TIGR01087 144 VLDQE-GAELYVLELSSFQL-ETTESLRPEIALILNISEDHLDWHGsfEDYVaAKLKIFARQTEG--DVAVLNADDPRFA 219

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  174 SKDIVNPVQYYGFDTakhapqlahyntegilcpkcEHILQYRLNTYanlgdficlncqfqrptldyqlteltaithqSSE 253
Cdd:TIGR01087 220 RLAQKSKAQVIWFSV--------------------EKDAERGLCIR-------------------------------DGG 248

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 407968025  254 FVIDGQNYKINVGGLYNIYNALAAVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFK 309
Cdd:TIGR01087 249 LYLKPNDLEGSLLGLHNAENILAAIALAKSLGLNLEAILEALRSFKGLPHRLEYVG 304
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 238-318 7.80e-11

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 63.58  E-value: 7.80e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 238 DYQLTELTAiTHQSSEFVI----DGQNYKINVGGLYNIYNALAAVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFKIGDk 313
Cdd:COG0770  245 DVRAEDIEL-DEDGTRFTLhtpgGELEVTLPLPGRHNVSNALAAAAVALALGLDLEEIAAGLAAFQPVKGRLEVIEGAG- 322


                 ....*
gi 407968025 314 SCTLI 318
Cdd:COG0770  323 GVTLI 327
murC TIGR01082
UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial ...
 38-296 3.25e-10

UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial peptidoglycan (murein) biosynthesis. In a few species (Mycobacterium leprae, the Chlamydia), the amino acid may be L-serine or glycine instead of L-alanine. A related protein, UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (murein tripeptide ligase) is described by model TIGR01081. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273433 [Multi-domain]  Cd Length: 448  Bit Score: 61.94  E-value: 3.25e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   38 KDILDSLSKDYEIVVITGTNGKTLTTALTVGILKEAFGDvltnPSGAnmITGITAAF-LSAKKGKSGKHIAvlEIDE--A 114
Cdd:TIGR01082  89 AEMLAELMRFRHSIAVAGTHGKTTTTAMIAVILKEAGLD----PTVV--VGGLVKEAgTNARLGSGEYLVA--EADEsdA 160

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  115 SLPKITnylkPSLFVYTNIFRDQMDRYGEIYTTY-QMIVDGARNAPKATIL-ANGDSPIFS--SKDIVNPVQYYGFDTAK 190
Cdd:TIGR01082 161 SFLHLQ----PNVAIVTNIEPDHLDTYGSSFERLkAAFEKFIHNLPFYGLAvICADDPVLRelVPKATEQVITYGGSGED 236

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  191 HAPQLAHYNTEGilcpkcehilqyrlntyaNLGDFiclncqfqrpTLDYQLTELTAIThqssefvidgqnykINVGGLYN 270
Cdd:TIGR01082 237 ADYRAENIQQSG------------------AEGKF----------SVRGKGKLYLEFT--------------LNLPGRHN 274

                         250       260
                  ....*....|....*....|....*.
gi 407968025  271 IYNALAAVSVAEFFGVSPEKIKAGFN 296
Cdd:TIGR01082 275 VLNALAAIAVALELGIDFEAILRALA 300
MurD COG0771
UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; ...
 50-294 6.43e-10

UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramoylalanine-D-glutamate ligase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440534 [Multi-domain]  Cd Length: 445  Bit Score: 60.86  E-value: 6.43e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  50 IVVITGTNGKTLTTALTVGILKEAFGDVltnPSGANmitgITAAFLSAKKGKSGKHIAVLE-----IDEaslpkiTNYLK 124
Cdd:COG0771  107 IIAITGTNGKTTTTTLIGHILKAAGLRV---AVGGN----IGTPLLDLLLEPEPPDVYVLElssfqLET------TPSLR 173

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 125 PSLFVYTNIFRDQMDRYGEI--YttyqmivdgaRNApKATILANGDSPIFSskdIVN-------------PVQYYGFDTA 189
Cdd:COG0771  174 PDVAVILNITPDHLDRHGSMeaY----------AAA-KARIFANQTPDDYA---VLNaddpltralaeeaKARVVPFSLK 239

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 190 KHAPQLAHYNtEGILCpkcehilqyrlntYANLGDFIClncqfqrpTLDyqlteltaithqssefvidgqnyKINVGGLY 269
Cdd:COG0771  240 EPLEGGAGLE-DGKLV-------------DRASGEELL--------PVD-----------------------DLRLPGRH 274

                        250       260
                 ....*....|....*....|....*
gi 407968025 270 NIYNALAAVSVAEFFGVSPEKIKAG 294
Cdd:COG0771  275 NLENALAALAAARALGVPPEAIREA 299
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 49-318 1.40e-09

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 59.59  E-value: 1.40e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   49 EIVVITGTNGKTLTTALTVGILKEAFGDVLTNPSGANMItGITAAFLSAkkgkSGKH-IAVLEI------DEASLPKItn 121
Cdd:TIGR01143  75 KVIGITGSSGKTTTKEMLAAILSHKYKVFATPGNFNNEI-GLPLTLLRA----PGDHdYAVLEMgashpgEIAYLAEI-- 147

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  122 yLKPSLFVYTNIFRDQMDRYG----------EIYttyqmivdgARNAPKATILANGDSPIFS--SKDIVN-PVQYYGFDT 188
Cdd:TIGR01143 148 -AKPDIAVITNIGPAHLEGFGslegiaeakgEIL---------QGLKENGIAVINADDPAFAdlAKRLPNrNILSFGFEG 217

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  189 AKHAPQLAHYNTEGilcpkCEHIlqyrlntyanlgDFICLNCQFqrptldyqlteltaithqssefvidgqNYKINVGGL 268
Cdd:TIGR01143 218 GDFVAKDISYSALG-----STSF------------TLVAPGGEF---------------------------EVSLPLLGR 253

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 407968025  269 YNIYNALAAVSVAEFFGVSPEKIKAGFNKSKAVFGRQETfkIGDKSCTLI 318
Cdd:TIGR01143 254 HNVMNALAAAALALELGIPLEEIAEGLAELKLVKGRFEV--QTKNGLTLI 301
murD PRK14106
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional
 50-306 2.81e-09

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional


Pssm-ID: 184511 [Multi-domain]  Cd Length: 450  Bit Score: 58.83  E-value: 2.81e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  50 IVVITGTNGKTLTTALTVGILKEAFGDVLTnpsGANmitgITAAFLSAKKGKSGKHIAVLEIDEASLPKItNYLKPSLFV 129
Cdd:PRK14106 110 IVAITGTNGKTTTTTLLGEIFKNAGRKTLV---AGN----IGYPLIDAVEEYGEDDIIVAEVSSFQLETI-KEFKPKVGC 181

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 130 YTNIFRDQMDRYGEIyttyqmivDGARNApKATILANGDSPIFSSKDIVNPVqyygfdTAKHAPQLahyntegilcpKCE 209
Cdd:PRK14106 182 ILNITPDHLDRHKTM--------ENYIKA-KARIFENQRPSDYTVLNYDDPR------TRSLAKKA-----------KAR 235

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 210 HILQYRLNTYANlGDFIclncqfqrptldyQLTELTAITHQSSEFVIDGQNYKINvgGLYNIYNALAAVSVAEFFGVSPE 289
Cdd:PRK14106 236 VIFFSRKSLLEE-GVFV-------------KNGKIVISLGGKEEEVIDIDEIFIP--GEHNLENALAATAAAYLLGISPD 299

                        250
                 ....*....|....*..
gi 407968025 290 KIKAGFNKSKAVFGRQE 306
Cdd:PRK14106 300 VIANTLKTFKGVEHRIE 316
Mur_ligase_M pfam08245
Mur ligase middle domain;
53-281 1.88e-08

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 54.23  E-value: 1.88e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025   53 ITGTNGKTLTTALTVGILKEAFGDVLT-----NPSGANMITGITAAFLSAKKGKSGKHIAVLEIDEASLPK--ITNYLKP 125
Cdd:pfam08245   1 VTGTNGKTTTTELIAAILSLAGGVIGTigtyiGKSGNTTNNAIGLPLTLAEMVEAGAEYAVLEVSSHGLGEgrLSGLLKP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  126 SLFVYTNIFRDQMDRYG---EIYTTYQMIVDGARNAPKATIlaNGDSPIFS-----SKDIVNPVQYYGFDTAKhapqlah 197
Cdd:pfam08245  81 DIAVFTNISPDHLDFHGtmeNYAKAKAELFEGLPEDGIAVI--NADDPYGAfliakLKKAGVRVITYGIEGEA------- 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  198 yntegilcpkcehilqyrlntyanlgdficlncqfqrptlDYQLTELTAiTHQSSEFVI---DGQNYKINVG--GLYNIY 272
Cdd:pfam08245 152 ----------------------------------------DLRAANIEL-SSDGTSFDLftvPGGELEIEIPllGRHNVY 190


                  ....*....
gi 407968025  273 NALAAVSVA 281
Cdd:pfam08245 191 NALAAIAAA 199
MurE COG0769
UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 238-313 2.63e-08

UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl tripeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440532 [Multi-domain]  Cd Length: 459  Bit Score: 55.86  E-value: 2.63e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 238 DYQLTELTaITHQSSEFVID--GQNYKINVG--GLYNIYNALAAVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFKIGDK 313
Cdd:COG0769  239 DLRATDIE-LSADGTRFTLVtpGGEVEVRLPliGRFNVYNALAAIAAALALGIDLEEILAALEKLKGVPGRMERVDGGQG 317
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 50-346 5.89e-08

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 55.10  E-value: 5.89e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025  50 IVVITGTNGKTLTTALTVGILKEAFGD--VLTNPSGANMITGITAAFLSAKKGKsgkHIAVLEIDEASLPKITnYL---- 123
Cdd:PRK11929 605 VVAITGSNGKTTTKEMIAAILAAWQGEdrVLATEGNFNNEIGVPLTLLRLRAQH---RAAVFELGMNHPGEIA-YLaaia 680

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 124 KPSLFVYTNIFRDQMDRYGEIyttyqmivDGarnapkatiLANGDSPIFSSKdIVNPVQYYGFD---TAKHAPQLAHYNT 200
Cdd:PRK11929 681 APTVALVTNAQREHQEFMHSV--------EA---------VARAKGEIIAAL-PEDGVAVVNGDdpyTAIWAKLAGARRV 742

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 407968025 201 egilcpkCEHILQYRLNTYANlgDFICLNCQFQRPTLDYQLTeltaITHQSSEFVIdgqnykiNVGGLYNIYNALAAVSV 280
Cdd:PRK11929 743 -------LRFGLQPGADVYAE--KIAKDISVGEAGGTRCQVV----TPAGSAEVYL-------PLIGEHNLRNALAAIAC 802

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 407968025 281 AEFFGVSPEKIKAGFNKSKAVFGRQETFKIgdkSCTLILI-----KNPVGASQALEMiqLADYPFSLSVLL 346
Cdd:PRK11929 803 ALAAGASLKQIRAGLERFQPVAGRMQRRRL---SCGTRIIddtynANPDSMRAAIDV--LAELPNGPRALV 868
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 238-313 4.00e-06

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 48.97  E-value: 4.00e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 407968025 238 DYQLTELTaITHQSSEFVIDGQnYKINVGGLYNIYNALAAVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFKIGDK 313
Cdd:PRK00139 249 DLRATDVE-YTDSGQTFTLVTE-VESPLIGRFNVSNLLAALAALLALGVPLEDALAALAKLQGVPGRMERVDAGQG 322
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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