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Conserved domains on  [gi|33356837]
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Chain B, PROTEIN : KETOSTEROID ISOMERASE

Protein Classification

steroid Delta-isomerase( domain architecture ID 10092371)

steroid Delta-isomerase catalyzes the interconversion of alpha-, beta- and beta, gamma-unsaturated ketosteroids via a dienolic intermediate

CATH:  3.10.450.50
EC:  5.3.3.1
Gene Ontology:  GO:0008202|GO:0004769

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 5-127 2.57e-67

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


:

Pssm-ID: 238404  Cd Length: 122  Bit Score: 199.19  E-value: 2.57e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837   5 TAQEVQGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFYRQGLGGGKvRACLTGPVRASHNGCGAM 84
Cdd:cd00781   1 TPQEMKAAVQRYVEAVNAGDPEGIVALFADDATVEDPVGSPPRSGRAAIAAFYAQSLGGAK-RLELTGPVRASHGGEAAF 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 33356837  85 PFRVEMVWNGQPCALDVIEVMRFDEHGRIQTMQAYWSEVNLSV 127
Cdd:cd00781  80 AFRVEFEWEGQPCVVRVIDVMRFDADGRIVSMRAYWGPVNLHV 122
 
Name Accession Description Interval E-value
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 5-127 2.57e-67

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 199.19  E-value: 2.57e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837   5 TAQEVQGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFYRQGLGGGKvRACLTGPVRASHNGCGAM 84
Cdd:cd00781   1 TPQEMKAAVQRYVEAVNAGDPEGIVALFADDATVEDPVGSPPRSGRAAIAAFYAQSLGGAK-RLELTGPVRASHGGEAAF 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 33356837  85 PFRVEMVWNGQPCALDVIEVMRFDEHGRIQTMQAYWSEVNLSV 127
Cdd:cd00781  80 AFRVEFEWEGQPCVVRVIDVMRFDADGRIVSMRAYWGPVNLHV 122
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
12-120 6.63e-11

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 55.70  E-value: 6.63e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837  12 LMARYIELVDVGDIEAIVQMYADDATVENPFGQP--PIHGREQIAAFYRQglgggkVRACLTG----PVRASHNGCGAMp 85
Cdd:COG3631   5 LVRRFYAAFNAGDVDALLALLAEDVVWEDPGGPGagTYRGKEAVRAFFAR------LAAAFEDlrfeVRRVVADGDRVV- 77

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 33356837  86 frVEMVW------NGQPCALDVIEVMRFDEhGRIQTMQAYW 120
Cdd:COG3631  78 --VEGTVrgtvlaTGKPYENRYADVFTVRD-GKIVRVREYF 115
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 15-113 8.99e-11

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 54.60  E-value: 8.99e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837    15 RYIELVDVGDIEAIVQMYADDATVENPFGqpPIHGREQIAAFYRQGLGG-GKVRACLTGPVRASHNGCGAMPFRVEMVWN 93
Cdd:pfam12680   3 RFYEALNAGDLDALAALFAPDAVFHDPGG--PLRGRDAIRAFFAALFAAfPDLRFEIHDVIADGDRVAVRWTVTGTIPPT 80

                          90       100
                  ....*....|....*....|
gi 33356837    94 GQPCALDVIEVMRFdEHGRI 113
Cdd:pfam12680  81 GRGVRVRGVDVFRF-RDGKI 99
PRK08241 PRK08241
RNA polymerase subunit sigma-70;
4-104 8.25e-09

RNA polymerase subunit sigma-70;


Pssm-ID: 236196 [Multi-domain]  Cd Length: 339  Bit Score: 52.25  E-value: 8.25e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837    4 PTAQEVQGLMARYIELVDVGDIEAIVQMYADDATVEnpfgQPPIH----GREQIAAFYRQGLGGGKVRACLTGPVRAshN 79
Cdd:PRK08241 211 PDDPEERALLARYVAAFEAYDVDALVALLTEDATWS----MPPFPlwyrGRDAIAAFLAGQCPGAGCGGSRLVPTRA--N 284

                         90       100
                 ....*....|....*....|....*....
gi 33356837   80 GCGA----MPFRVEMVWngQPCALDVIEV 104
Cdd:PRK08241 285 GQPAfaqyMRDPDGGGH--RPWALHVLEL 311
TIGR02096 TIGR02096
conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about ...
 10-57 5.22e-06

conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about 135 amino acids in length largely restricted to the Proteobacteria. This family and a delta5-3-ketosteroid isomerase from Pseudomonas testosteroni appear homologous, especially toward their respective N-termini. Members, therefore, probably are enzymes.


Pssm-ID: 273970  Cd Length: 129  Bit Score: 42.75  E-value: 5.22e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 33356837    10 QGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFY 57
Cdd:TIGR02096   1 TELAQHWIEAFNRGDMDAVLALLAEDVLYDDNQGGRVLGGKAQLARFL 48
 
Name Accession Description Interval E-value
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 5-127 2.57e-67

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 199.19  E-value: 2.57e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837   5 TAQEVQGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFYRQGLGGGKvRACLTGPVRASHNGCGAM 84
Cdd:cd00781   1 TPQEMKAAVQRYVEAVNAGDPEGIVALFADDATVEDPVGSPPRSGRAAIAAFYAQSLGGAK-RLELTGPVRASHGGEAAF 79

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 33356837  85 PFRVEMVWNGQPCALDVIEVMRFDEHGRIQTMQAYWSEVNLSV 127
Cdd:cd00781  80 AFRVEFEWEGQPCVVRVIDVMRFDADGRIVSMRAYWGPVNLHV 122
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 9-120 2.62e-12

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 59.07  E-value: 2.62e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837   9 VQGLMARYIELVDVGDIEAIVQMYADDATVENPFGQ---PPIHGREQIAAFYRQGLGGGKVRACLTGPVRASHNG----- 80
Cdd:cd00531   1 AEQFLYRYARLLDAGDREWLALLYADDAYFEPPGGDgliYPDDGREAIEDRVRRLPFGPSRTRHLVSNVDVQPGDdgegv 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 33356837  81 CGAMPFRVEMVWNGQPCALDVIEVMRF---DEHGRIQTMQAYW 120
Cdd:cd00531  81 VVSVFGVLRTRGDGEQDVFAGGQTFVLrpqGGGGKIANRRFRL 123
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
12-120 6.63e-11

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 55.70  E-value: 6.63e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837  12 LMARYIELVDVGDIEAIVQMYADDATVENPFGQP--PIHGREQIAAFYRQglgggkVRACLTG----PVRASHNGCGAMp 85
Cdd:COG3631   5 LVRRFYAAFNAGDVDALLALLAEDVVWEDPGGPGagTYRGKEAVRAFFAR------LAAAFEDlrfeVRRVVADGDRVV- 77

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 33356837  86 frVEMVW------NGQPCALDVIEVMRFDEhGRIQTMQAYW 120
Cdd:COG3631  78 --VEGTVrgtvlaTGKPYENRYADVFTVRD-GKIVRVREYF 115
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 15-113 8.99e-11

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 54.60  E-value: 8.99e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837    15 RYIELVDVGDIEAIVQMYADDATVENPFGqpPIHGREQIAAFYRQGLGG-GKVRACLTGPVRASHNGCGAMPFRVEMVWN 93
Cdd:pfam12680   3 RFYEALNAGDLDALAALFAPDAVFHDPGG--PLRGRDAIRAFFAALFAAfPDLRFEIHDVIADGDRVAVRWTVTGTIPPT 80

                          90       100
                  ....*....|....*....|
gi 33356837    94 GQPCALDVIEVMRFdEHGRI 113
Cdd:pfam12680  81 GRGVRVRGVDVFRF-RDGKI 99
YybH COG4319
Ketosteroid isomerase homolog YybH [General function prediction only];
1-64 3.99e-10

Ketosteroid isomerase homolog YybH [General function prediction only];


Pssm-ID: 443460 [Multi-domain]  Cd Length: 131  Bit Score: 53.61  E-value: 3.99e-10
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 33356837   1 MNLPTA--QEVQGLMARYIELVDVGDIEAIVQMYADDATVENPFGqPPIHGREQIAAFYRQGLGGG 64
Cdd:COG4319   1 MKRSAEdeAAIRALLAAFAEAFNAGDADALAALYAEDAVFFDPGG-PPVRGREAIRAAWAAAFAAG 65
PRK08241 PRK08241
RNA polymerase subunit sigma-70;
4-104 8.25e-09

RNA polymerase subunit sigma-70;


Pssm-ID: 236196 [Multi-domain]  Cd Length: 339  Bit Score: 52.25  E-value: 8.25e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837    4 PTAQEVQGLMARYIELVDVGDIEAIVQMYADDATVEnpfgQPPIH----GREQIAAFYRQGLGGGKVRACLTGPVRAshN 79
Cdd:PRK08241 211 PDDPEERALLARYVAAFEAYDVDALVALLTEDATWS----MPPFPlwyrGRDAIAAFLAGQCPGAGCGGSRLVPTRA--N 284

                         90       100
                 ....*....|....*....|....*....
gi 33356837   80 GCGA----MPFRVEMVWngQPCALDVIEV 104
Cdd:PRK08241 285 GQPAfaqyMRDPDGGGH--RPWALHVLEL 311
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 8-58 1.30e-08

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


Pssm-ID: 396625  Cd Length: 116  Bit Score: 49.27  E-value: 1.30e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 33356837     8 EVQGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFYR 58
Cdd:pfam02136   1 VGNAFVQQYYAALDAHDPEGLHALYADDASMLTPPGSDPVVGLEAINEFFD 51
SnoaL_4 pfam13577
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 8-66 7.55e-07

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 433323 [Multi-domain]  Cd Length: 125  Bit Score: 45.02  E-value: 7.55e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 33356837     8 EVQGLMARYIELVDVGDIEAIVQMYADDATVENPfGQPPIHGREQIAAFYRQGLGGGKV 66
Cdd:pfam13577   8 AIRDLVARYGRALDTGDWDELAALFTEDAVFDYG-GLGVLEGRDAIVAGLRAALDGFLL 65
TIGR02096 TIGR02096
conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about ...
 10-57 5.22e-06

conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about 135 amino acids in length largely restricted to the Proteobacteria. This family and a delta5-3-ketosteroid isomerase from Pseudomonas testosteroni appear homologous, especially toward their respective N-termini. Members, therefore, probably are enzymes.


Pssm-ID: 273970  Cd Length: 129  Bit Score: 42.75  E-value: 5.22e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 33356837    10 QGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFY 57
Cdd:TIGR02096   1 TELAQHWIEAFNRGDMDAVLALLAEDVLYDDNQGGRVLGGKAQLARFL 48
SnoaL COG4538
Uncharacterized conserved protein, contains SnoaL-like domain [Function unknown];
2-59 1.56e-04

Uncharacterized conserved protein, contains SnoaL-like domain [Function unknown];


Pssm-ID: 443604  Cd Length: 113  Bit Score: 38.33  E-value: 1.56e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 33356837   2 NLPTAQE-VQGLMARYielvDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFYRQ 59
Cdd:COG4538   1 ATMTPEDvVQRQLDAY----NARDIDAFLATYADDAEVYRFPGTLLAKGREAIRERYAE 55
TIGR02246 TIGR02246
conserved hypothetical protein; This family consists of uncharacterized proteins found in a ...
 6-59 7.51e-04

conserved hypothetical protein; This family consists of uncharacterized proteins found in a number of genera and species, including Streptomyces, Xanthomonas, Oceanobacillus iheyensis, Caulobacter crescentus CB15, and Xylella fastidiosa. The function is unknown.


Pssm-ID: 274053  Cd Length: 128  Bit Score: 36.97  E-value: 7.51e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 33356837     6 AQEVQGLMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIhGREQIAAFYRQ 59
Cdd:TIGR02246   3 ERAIRALVARWEAAWAAGDAEAFADLFAPDGVFVTVPGQVRK-GREAIRAAHEA 55
CaMKIID COG4875
Protein kinase association domain CaMKII_AD, NTF2-like superfamily [Posttranslational ...
 4-59 1.05e-03

Protein kinase association domain CaMKII_AD, NTF2-like superfamily [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 443903  Cd Length: 156  Bit Score: 36.91  E-value: 1.05e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837   4 PTAQEVQGLMARYIELVDVGDIEAIVQMYADDA----TVENpfgqPPIHGREQIAAFYRQ 59
Cdd:COG4875  33 ATEAQIAALFDRWNAALQTGDADKVLALYAPDAvllpTVSN----KVRTTRAEIRDYFEH 88
DUF4440 pfam14534
Domain of unknown function (DUF4440);
14-60 2.87e-03

Domain of unknown function (DUF4440);


Pssm-ID: 434023 [Multi-domain]  Cd Length: 107  Bit Score: 35.07  E-value: 2.87e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 33356837    14 ARYIELVDVGDIEAIVQMYADDATVENPFGqpPIHGREQIAAFYRQG 60
Cdd:pfam14534   6 EALLEALVAGDPAALAALLAPDFVLVGPSG--PVLDKDEILEALASG 50
SnoaL_3 pfam13474
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 12-65 4.62e-03

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 433238  Cd Length: 121  Bit Score: 34.63  E-value: 4.62e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 33356837    12 LMARYIELVDVGDIEAIVQMYADDATVENPFGQPPIHGREQIAAFYRQGLGGGK 65
Cdd:pfam13474   4 LNDAWYEAAAEGDFDALFALWSDDISCIGTGAGELWRGREEFRRSWKQDFAQAQ 57
COG5485 COG5485
Predicted ester cyclase [General function prediction only];
12-120 6.92e-03

Predicted ester cyclase [General function prediction only];


Pssm-ID: 444236 [Multi-domain]  Cd Length: 130  Bit Score: 34.06  E-value: 6.92e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 33356837  12 LMARYIELV-DVGDIEAIVQMYADDATVENPFGQPPiHGREQIAAFYRQGLG---------------GGKVRACLTgpVR 75
Cdd:COG5485   1 LVRRFFEEAwNEGDLDALDELVAPDFVYHGPLPGEP-RGPEGFRAFLRGLRAafpdlrfeiedliaeGDRVAVRWT--FT 77

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 33356837  76 ASHNGcgamPFRVEMVwNGQPCALDVIEVMRFDEhGRIQTMQAYW 120
Cdd:COG5485  78 GTHTG----EFLGIPP-TGKRVEFRGIDIYRFED-GKIVEEWVVE 116
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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