2GDU


Conserved Protein Domain Family
AmyAc_Sucrose_phosphorylase

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cd11355: AmyAc_Sucrose_phosphorylase 
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Alpha amylase catalytic domain found in sucrose phosphorylase (also called sucrose glucosyltransferase, disaccharide glucosyltransferase, and sucrose-phosphate alpha-D glucosyltransferase)
Sucrose phosphorylase is a bacterial enzyme that catalyzes the phosphorolysis of sucrose to yield glucose-1-phosphate and fructose. These enzymes do not have the conserved calcium ion present in other alpha amylase family enzymes. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
Statistics
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PSSM-Id: 200492
Aligned: 24 rows
Threshold Bit Score: 709.768
Created: 10-Oct-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                         #  #                           
2GDU_A         2 KNKVQLITYADRLGdGTIKSMTDILRTRFDG-VYDGVHILPFFTPFDgaDAGFDPIDHTKVDERLGSWDDVAELSKTHNI 80  Bifidobacterium...
YP_004092044   4 KNQVMLITYPDSLG-RNLKDLRAILKRYFDG-VVGGVHILPFFPSSG--DRGFSPVTYKKVSPEFGTFEDIGALAQEYYL 79  Ethanoligenens ...
YP_003802639   2 KNGVQLITYPDSLG-INLEELSTALERYFPD-LFTGIHLLPFYPSSA--DRGFAPLGYDRVDPQFGDWNAVEGLAGRYDI 77  Spirochaeta sma...
CBK74036       5 ENKCMLITYADSMG-DNLSDLTTVMNKHFKD-AVGGIHILPFFPSSG--DRGFAPMDYHVVDPAFGDWNDIEKLAEDYYL 80  Butyrivibrio fi...
ZP_08554373    2 ENKIMLITYADSMG-KDLKDLNNVLNNHFKD-TIGSVHILPFYPSSA--DRGFAPMTYTEVDEVFGDWKDILKISENYEL 77  Haloplasma cont...
ZP_08336178    4 ENKVMLITYADSMG-KNLKELHTVLNTYYRD-AVGGVHILPFFPSSA--DRGFAPMRYDVVDEAFGTYEDIEAIGREYYL 79  Lachnospiraceae...
ZP_01168805    4 KNQVQLITYPDSLG-GDLKMLNKVMTEHFADiFKGGVHILPPFPSSG--DRGFAPLTYLEIDPEFGGWEDIRKIGENFDI 80  Bacillus sp. NR...
ZP_08605244    2 KNKIMLITYSDSFG-KNLKELKQMVDTYFKR-EINGIHILPFFPSSG--DRGFAPINYREVDPQFGTWEDIQALAEDYEL 77  Lachnospiraceae...
YP_003852491   4 KNKVQLITYPDSLG-GNLKTLNDVLEKYFSD-VFGGVHILPPFPSSG--DRGFAPITYSEIEPKFGTWYDIKKMAENFDI 79  Thermoanaerobac...
YP_001528538   4 RNRIQVISYPDSLG-KNLQELHYVLRRYFAD-AAGGVHLLPFYPSSA--DRGFAPVTYYQVDPAFGTWRDIELIQEDFDI 79  Desulfococcus o...
Feature 1               #                                                                        
2GDU_A        81 MVDAIVNHMSWESKQFQDVLAKGEESEYYPMFLt-mSSVFPNGATEEDLAGIYRPRPGlPFTHYKfa------gKTRLVW 153 Bifidobacterium...
YP_004092044  80 MFDYMLNHISAQSAYYQDFLKNKDASLWKDLFIrykIFWKNSFPTQEQVDVIYKRKPRaPYVDAHft-----dgTEEKVW 154 Ethanoligenens ...
YP_003802639  78 VVDFMVNHISRHSPQFLDFLKRGEKSLWADLFLnvrKFHPSGEIPPDDLAKIYTRKPRaPLTKVVhp-----dgSEDTLW 152 Spirochaeta sma...
CBK74036      81 MADYMINHISAQSEYYKDFLAKKDDSKYKDLFIrykDFWENGEPTEEEVDAIYKRKPRaPYVDANfa-----dgTTEKVW 155 Butyrivibrio fi...
ZP_08554373   78 MYDFMINHISRSSKYFQDFLKNKDESSYSDLFIrykDFWKNGEPTEEQVDKIYKRKPRaPYVIATfe-----dgSKEKVW 152 Haloplasma cont...
ZP_08336178   80 MFDFMVNHISSSSEYFQDFLKRKNESKYADFFIryeEFWGKGAPTQEQLDKIYKRKPRaPYIEVEfq-----dgTTEKIW 154 Lachnospiraceae...
ZP_01168805   81 LVDLMVNHISQKSEYFQDFLAKGRQSQYADLFItldKLWEDGQPVQEDIDKMFLRRPL-PYSTFTieetgeqetVWTTFG 159 Bacillus sp. NR...
ZP_08605244   78 MFDFMINHLSRRSPEFLDFLEKHDQSEYADMFLrfkKFWPNGEPDQKQVDMLNKRKPCaPCEDILfa-----dgTTEKIW 152 Lachnospiraceae...
YP_003852491  80 LLDLMVNHVSRRSIYFQDFLKKGRKSEYADMFItldKLWKDGKPVKGDIEKMFLRRTL-PYSTFKieetgeeekVWTTFG 158 Thermoanaerobac...
YP_001528538  80 TIDFMVNHISRQSKYVADFIEKGDKSEYADMFLsfnKLAPDGEIRKEDLDKVYTRKPSpPYQVIErp-----dgSRETIW 154 Desulfococcus o...
Feature 1          #   #                             # ##  #                                    #
2GDU_A       154 VSFTPQQVDIDTDSDKGWEYLMSIFDQMAASHVSYIRLDAVGYGAKEAGTSCFMT-PKTFKLISRLREEGVKRGLEILIQ 232 Bifidobacterium...
YP_004092044 155 CTFSEEQIDLNVRSTVTKRFNRNNLEFLVKQGASIIRLDALAYVTKRPGTSCFFIePDIWNILNEYSEILKPFHVDILPE 234 Ethanoligenens ...
YP_003802639 153 CTFSEEQIDLDIASPVGTAFLEDNLRSLCGHGISMLRLDAFAYVTKRLGTNCFFVePDVWELLHRIQTIVAEYGVEILPE 232 Spirochaeta sma...
CBK74036     156 CTFDEQQIDINCKSATAKEFIKDNLETLCKHGLAMIRLDAFAYATKKAGSSCFFIePEVWELMDESAKIVEPYGVTLLPE 235 Butyrivibrio fi...
ZP_08554373  153 CTFDEEQIDLDVTTDTTKQFVIDNLSFLAKHGANFIRLDAFAYAIKKPDTSCFFIePGVWELLDECQAILKPMGVEMLPE 232 Haloplasma cont...
ZP_08336178  155 CTFCEEQIDLDVRKEAVKSFIKGTLEQMCQNGASLIRLDAFAYAIKKADTNCFFVePDIWELLHEIEQIVGRYEVEILPE 234 Lachnospiraceae...
ZP_01168805  160 KTDPSEQIDLDIKSEQVRQLLTDFFTNFKEQNVKIVRLDAVGYVVKKLGTSCFFVePEIYEFLDWIMELANSLGIELLPE 239 Bacillus sp. NR...
ZP_08605244  153 CTFDNEQMDLNLESETAWRFVEKTLRFLMDQGAAMIRLDAFAFATKKLNTSCFFIePEMWDMMKRVQGILDEKNIPMLPE 232 Lachnospiraceae...
YP_003852491 159 KTDPSEQIDLDVNSHLVREFLLEVFKTFSNFGVKIVRLDAVGYVIKKIGTSCFFVePEIYEFLDWAKGQAASYGIELLLE 238 Thermoanaerobac...
YP_001528538 155 STFDYEQIDVDVQSPVTRELFRDFLIFLSRRKPEFIRMDAFAYTTIRLGTNCFFVePEVWELLEMLEGFVSPFGVQLLPE 234 Desulfococcus o...
Feature 1         #                                                      ##                      
2GDU_A       233 VHSYYKKQVEIASKVDRVYDFALPPLLLHALSTGHVEPVAHWTDIRPNNAVTVLDTHDGIGVIDIgsdqldrslKGLVPD 312 Bifidobacterium...
YP_004092044 235 IHEHYSIQLKLAEHGNWVYDFSLPMLVLHALYSGLAERLVHWLEICPRKQFTTLDTHDGIGVVDV---------KSLLTD 305 Ethanoligenens ...
YP_003802639 233 IHEHYSIQLKLANKGYRVYDFALPMLLLHTIFSGSSRRLKEWLKISPHNQITTLDTHDGIGVVDA---------ADLLSS 303 Spirochaeta sma...
CBK74036     236 IHEHYTMQQKIESHDYYVYDFALPMLLINGLYYGQSQYLKHWLEICPRKQFTTLDTHDGIGVVDV---------KDLLPD 306 Butyrivibrio fi...
ZP_08554373  233 IHEHYTIQLNMAKKGCWVYDFALPMLVLYSLFSGKNNRLINWLNICPRKQFTTLDTHDGIGVVDV---------VDLMTD 303 Haloplasma cont...
ZP_08336178  235 IHEHYTIPMKIAEKGFWVYDFALPVLTLHALYNHTGVYLKKWLEMCPMKQFTTLDTHDGIGIVDV---------KDLLPD 305 Lachnospiraceae...
ZP_01168805  240 VHSHYSIQHKLAEHGFWIYDFILPYRVLETLINKSSKNLAIYLKNRPHKQFTMLDCHDGIPVKPDl--------DGLIDT 311 Bacillus sp. NR...
ZP_08605244  233 IHDHYTVQQKIADHGYTVYDFVLPVMVLHTLFSQSSRRLRHWLEICPRDQHTTLDTHDGLGTVDV---------EDLLSE 303 Lachnospiraceae...
YP_003852491 239 VHSQFEVQYKLAERGFLIYDFILPFTVLYTLINKSNEMLYHYLKNRPINQFTMLDCHDGIPVKPDl--------DGLIDT 310 Thermoanaerobac...
YP_001528538 235 VHEHYSYQLKLAEKGYWVYDFALPMLVLHTLYHHTNKRLLHWLRICPRRQFTTLDTHDGLGVVDV---------ADLMSQ 305 Desulfococcus o...
Feature 1                                      ##  #                                             
2GDU_A       313 EDVDNLVNTIHANTHGESQAA--TGAAASNLDLYQVNSTYYSALGXNDQHYIAARAVQFFLPGVPQVYYVGALAGKNDME 390 Bifidobacterium...
YP_004092044 306 KEIDFTIKALYEHGANIKQKY--SSAEYNNLDIYQINCTYYSALGNDDAAYLLARVIQLFAPGIPQVYYVGMLAGKNDVD 383 Ethanoligenens ...
YP_003802639 304 EEIEKTVDALYERGSNVNRRY--SSEEYNNLDIYQINCTYYSATGEDDDAYILSRAIQFFAPGIPQVYYVGALSGRNDTD 381 Spirochaeta sma...
CBK74036     307 EEIERTKEDIFKFGANVKKIY--NTAAYNNLDIYQVNCSYYSALGDDDDAYLLARAVQFFAPGIPQVYYVGLLAGKNDLQ 384 Butyrivibrio fi...
ZP_08554373  304 KEIDYTKEVLYKKGANVKKKY--SSAEYKNLDIYQINCTYYSALGNNDAAYLLARAIQFFAPGIPQVYYVGLLAGENDLE 381 Haloplasma cont...
ZP_08336178  306 EEVYIVQEQMYRQGANVKKIY--SSESYNNLDVYQVNTTYYSALGNRDDAYLLARAIQFFAPGIPQVYYVGLLAGENDLE 383 Lachnospiraceae...
ZP_01168805  312 KEAKDIVDVCLERGSNLSLILsdEHKAEDGFDVHQIRCSYYSVLNSDDDAYLAARAIQFFTPGVPQVYYVGLLAGENDVE 391 Bacillus sp. NR...
ZP_08605244  304 EELQAVIDKTEEYGANFKWDY--SQKGHGGKRVYQINCSYYSAVGEDDDSYLLSRAIQFFTPGVPQVYYMGLLAGENDYE 381 Lachnospiraceae...
YP_003852491 311 KKAKEVVDICVQRGANLSLIYgdKYKSEDGFDVHQINCTYYSALNCDDDAYLAARAIQFFTPGIPQVYYVGLLAGVNDFE 390 Thermoanaerobac...
YP_001528538 306 EEIERTVDGLYEKGSNIKRIY--STADYQNLDIYQVNCTYYSALERNDDSYLAARTLQFYAPGIPHVYYVGLLAGENDVA 383 Desulfococcus o...
Feature 1                #                                           
2GDU_A       391 LLRKTNNGRDINRHYYSTAEIDENLKRPVVKALNALAKFRNELDAFDGTFSY 442 Bifidobacterium adolescentis
YP_004092044 384 LLGRTKEGRNINRHYYTEEEIATETQRPLLKKLFRLMRFRNSYGAFEGTVSI 435 Ethanoligenens harbinense YUAN-3
YP_003802639 382 LIEQTKQGRDINRHPYGLDEIAVEIERPVNKRLFRLMRFRSHTPAFEGSFSL 433 Spirochaeta smaragdinae DSM 11293
CBK74036     385 LLEETKVGRNINRHYYKLDEIDREVERPVVKKLLELMKFRNEHSAFDGEFIL 436 Butyrivibrio fibrisolvens 16/4
ZP_08554373  382 LLEKTKVGRNINRHYYTVDEINERVTDPMLQNMYKLMEFRNTYDAFNGDVTI 433 Haloplasma contractile SSD-17B
ZP_08336178  384 LMEKTKNGRDINRHYYTLEEIAGEQERPVVRKLKKLMAIRNMEPAFHLDGKI 435 Lachnospiraceae bacterium 9_1_43BFAA
ZP_01168805  392 NVARTGEGREINRHNFTLEEIEESLEKPVVQRLLKLIRFRNENEAFSGEFQV 443 Bacillus sp. NRRL B-14911
ZP_08605244  382 LMERTQYDRNISRHNYTVEEIETLVEKPVVKKLCRLMQFRNEYPVFDGEMSL 433 Lachnospiraceae bacterium 3_1_57FAA_CT1
YP_003852491 391 AVKKTKEGREINRHNYGLKEIEESVQKNVVQRLLKLIRFRNEYEAFNGEFFI 442 Thermoanaerobacterium thermosaccharolyticum...
YP_001528538 384 LVEATRNGRDINRHNYTLDEIAVAVEREVVQRLIRLMRFRSSYPAFDGELVI 435 Desulfococcus oleovorans Hxd3

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