1EV7,1IAW


Conserved Protein Domain Family
NaeI-like

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cd22338: NaeI-like 
Click on image for an interactive view with Cn3D
Restriction endonuclease NaeI and similar endonucleases
The type II restriction endonuclease NaeI recognizes and cleaves the DNA motif GCC|GGC (| denotes the cleavage site) and forms a covalent bond with the cleaved substrate. The enzyme binds two DNA recognition sites and only cleaves one DNA sequence. It belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Statistics
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PSSM-Id: 411742
Aligned: 55 rows
Threshold Bit Score: 190.503
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1IAW: Lentzea aerocolonigenes restriction endonuclease NaeI active site binds substrate DNA; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        #
1EV7_A         13 DDLERVRATLYsldpdgdrtaGVLRDTLDQLYDGQRTGRWNFdqlHKTEKTHMGTLVEINLHREFQFg------DGFETD 86   Lechevalieria...
1IAW_A         13 DDLERVRATLYsldpdgdrtaGVLRDTLDQLYDGQRTGRWNFdqlHKTEKTHMGTLVEINLHREFQFg------DGFETD 86   Lechevalieria...
WP_051695906 1309 EELEALHSSLLaadpdgsvlaYALRDAFDFVLDGTRTGRFDVrelSRAERSVIGVKVEQAITSALALe------RGVRMD 1382 Streptomyces ...
WP_051366001  234 ADLEALARALLrldpdgtrfaAVLRRSLDQMYDGQHTGRFSWeqmTRIEKSSLSARIELELMREFSLv------SGGRSD 307  Hamadaea tsun...
OWA17377     1260 SELNILYATLLaadplgnhtgWVLRDAFDFVLDGARTHRFDMlalSPAERAVVGVKVEQMITSLFALg------AGREAD 1333 Streptomyces ...
SOB88669      281 TDLQAVVLELErqdpvgqrmaSILVAAMDRVIDGPRTGRFHLgqiSALERSSLHAITANLIQHEFGFp------DGHPTD 354  Streptomyces ...
BBH63723      228 PALDVLEEQLRgldpdgallaAVIRRALDHQYDGPHTGRYAWeqlTKWEKAAVGAKVEVEFQRAYDLa------DGGDAD 301  Actinoplanes ...
KKZ70868       15 GELLALARVFSeldpegrrfaGILRDSLDAVLDARNTGRFDPeelRIDEFARIPWAVKEGLQSVFTFv------PGRTAD 88   Streptomyces ...
WP_010983774 1360 SELLRLARTFNeldpdgrrlaGALRDCFDAALDMENTGRFDLrqlRGRERALTALELERSLCRAFPFd------AGESTD 1433 Streptomyces ...
WP_051739612  198 AGLSTVARTLQsldpdgsrlvSCIRRTFDQVLDGPRTGRFRLdqlSRSERMRLPLQMETEVEREFGFasnrslsVGHGAD 277  Streptomyces ...
Feature 1                       # #                                                               
1EV7_A         87 Yei------agVQVDCKFSmsqGAWMLPPEsi---gHICLVIWAsdqqcAWTAGLVKVIpq-fLGTANRd--LKRRLTPe 154  Lechevalieria...
1IAW_A         87 Yei------agVQVDCKFSmsqGAWMLPPEsi---gHICLVIWAsdqqcAWTAGLVKVIpq-fLGTANRd--LKRRLTPe 154  Lechevalieria...
WP_051695906 1383 Lrh------rgTEFDLRFSgrrGVWAIPPEty---gHICLLIHAddlhsRWGAGLLRIDpd-lVLRPAGn-rDGNRALRr 1451 Streptomyces ...
WP_051366001  308 Lel------agIAFDVKFSr-rSDWLFSRRd----sGLCLLVTGddgesRVSVALVRVTad-iLRAGGNr--DGKAALSa 373  Hamadaea tsun...
OWA17377     1334 Lsl------dgIDFDLRFStrfGAWMFPREsy---gRIQLLVHAddrtsRWSAGLVHVApetdLGSAYNr--DLKRALRk 1402 Streptomyces ...
SOB88669      355 Flv------agIQVRYRFSt-lQGWGIPASal---nQVCLLVEVgeegrHWNMGIVRATpe-hLGSYGNr--DGKRHLSa 421  Streptomyces ...
BBH63723      302 Fal------agVEFDLKYStrdRGWVIDQWrl---eVPQLLITGndhqsVMSAGLLRPHpg-lLNRVGR---DGRATLSt 368  Actinoplanes ...
KKZ70868       89 Lvwqr--pqgdVPFDLKIS---FRRPRPRFdrnavgGVSLIVYAddaraRWSAGLVRVRpe-hLAPGANr--DGTSPLTa 160  Streptomyces ...
WP_010983774 1434 LvwrpsgrrapVPFELRLSltgPNWRFSPHtq---dVLCLVVQAddrasRWSAGLIRVRpd-aLTRTSEpenDGTAVLTt 1509 Streptomyces ...
WP_051739612  278 Fyv------egVPVDFRFRvgrIGRVRSFGp-----DPVLLVHAddgrgLWSAGLWCDP----QAGGAA---LLGRELQ- 338  Streptomyces ...
Feature 1                                                                                         
1EV7_A        155 g-raQVVKLWPDHGKLQENLLLhipGDVRDQIFSakssrgnqHGQARVNELFRRVHg--RLIGRAVIATVAQQd-DFMKR 230  Lechevalieria...
1IAW_A        155 g-raQVVKLWPDHGKLQENLLLhipGDVRDQIFSakssrgnqHGQARVNELFRRVHg--RLIGRAVIATVAQQd-DFMKR 230  Lechevalieria...
WP_051695906 1452 t-amDRVRWLHQDMPLPENVLLhldPDARAAVLTp------rGPQRRVNELFRRAIg--KRITVTTLTMVAGP--SGHRR 1520 Streptomyces ...
WP_051366001  374 d-grAALQWLHRDVSLPENTLLhlpTRDREAILGt------tPGQRRLNEFFRRVPg--RPISRTVVATLVTQt-DSAKR 443  Hamadaea tsun...
OWA17377     1403 d-arHKVRWLHRDAPLPENVLLhlpPHDREAVLAa------pTGQQRVEELFRRAIg--RPITMATLSTLAGP--NAARR 1471 Streptomyces ...
SOB88669      422 t-gvSAVRWLHREAMLPISVMAdlpAATLENITCh------kNAQVRVDNLFRSIQg--RPIDRQTIVALARSaiHGERR 492  Streptomyces ...
BBH63723      369 e-glKAVRWLHREVPLPENTLAhltPDIRERVLSh------rSGQQRVVELFRLVQd--RPISTVTVQTVALQq-DAMKR 438  Actinoplanes ...
KKZ70868      161 eahrGAVLWLHRDAPLPENLLLhldPEDREAILAg------pDDVSRVAELFRRVRf--RTVPASALRALIRAt-DPMRT 231  Streptomyces ...
WP_010983774 1510 sarrSAVLWLRRDAPLPENVLLhldDATRAAILAp------kDAVGRTAEFFRRVQg--RPVPVTALRPLLRGq-DHKRR 1580 Streptomyces ...
WP_051739612  339 ----DTMHWLFRDAPLPGNVLLqlpADDLQAIFApgs--ahgRIVELMRRAPGRLVsrsALKTVGMTSDVDRRirRVRAR 412  Streptomyces ...
Feature 1                                                                         
1EV7_A        231 VRGsg------gARSILRPEGIIILGHQdndpk-vandlGLPVPRk-----------gQVVAAR 276  Lechevalieria aerocolonigenes
1IAW_A        231 VRGsg------gARSILRPEGIIILGHQdndpk-vandlGLPVPRk-----------gQVVAAR 276  Lechevalieria aerocolonigenes
WP_051695906 1521 ARE---------ARRLLEPEGIAVLGPR-----------DLPPDGysap------alnEWMSVR 1558 Streptomyces sp. NRRL S-244
WP_051366001  444 VRD---------ARRALKEEGYLVLGSAyanhr--giatDLGLSLee---------pgSYMSVR 487  Hamadaea tsunoensis
OWA17377     1472 ARE---------ARTRLAPEGITIRSTRdfstrhdmfqsDRSPDAa------------STDDQT 1514 Streptomyces sp. CS057
SOB88669      493 ARE---------SRARLVPEGILVLTLRddq-----lisDLQLPAlg---------psQWLSIR 533  Streptomyces sp. 1331.2
BBH63723      439 VRD---------ASRQLDGEGVRILSGNrlgdq--riaaDLGLPAlr---------pgSFMSIR 482  Actinoplanes sp. OR16
KKZ70868      232 VRL---------AGAALREEGLLLLTGTprgra-taetlRLPVPEp-----------dEFVCAP 274  Streptomyces showdoensis
WP_010983774 1581 VRE---------AVVVLRPEGVLVVRGNswgra-vaealRLRRPAe-----------dELLSVR 1623 Streptomyces avermitilis
WP_051739612  413 LADdgiviltwfDRDLAAQLGLPQPSPReyv------saRLARVApgrtdvptaelegERWTVA 470  Streptomyces catenulae

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