1IAW: Crystal Structure Of Naei Complexed With 17mer Dna

NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.
PDB ID: 1IAWDownload
MMDB ID: 72350
PDB Deposition Date: 2001/3/23
Updated in MMDB: 2012/08
Experimental Method:
x-ray diffraction
Resolution: 2.4  Å
Source Organism:
Lechevalieria aerocolonigenes
Similar Structures:
Biological Unit for 1IAW: hexameric; determined by author
Molecular Components in 1IAW
Label Count Molecule
Proteins (2 molecules)
Type II Restriction Enzyme Naei
Molecule annotation
Nucleotide(1 molecule)
5'-d(tpgpcpcpapcpgpcpcpgpgpcpgptpg 3'
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB