PUA RNA-binding domain of eukaryotic translation initiation factor 2D and similar proteins.
The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Most members of this eukaryotic translation initiation factor 2D (eIF2d)-like family of eukaryotic proteins also contain a domain homologous to the translation initiation factor eIF1/SUI1, and a short uncharacterized N-terminal domain. eIF2D may function as a cytosolic GTP-independent initiation factor which delivers Met-tRNA (and non-initiating tRNAs) to the 40S ribosomal subunit. The family member from Drosophila melanogaster has been named ligatin, and this alias has been adopted for other family members as well, which are not homologous to the vertebrate ligatin (LGTN) that is a trafficking receptor for phosphoglycoproteins.
Feature 1:RNA binding site [nucleic acid binding site]
Evidence:
Comment:many PUA domains have been shown to bind RNA, and although detailed interactions vary, they all seem to share a common surface patch that is associated with binding
Structure:5OA3: PUA domain of human translation initiation factor 2D binds initiator tRNA, contacts at 4A
Jiyao W et al.(2023). "The conserved domain database in 2023.",