PUA RNA-binding domain of archaeosine tRNA-guanine transglycosylase
The RNA-binding PUA (PseudoUridine synthase and Archaeosine transglycosylase) domain was detected in a number of proteins involved in RNA metabolism. Members of this archaeosine tRNA-guanine transglycosylase (TGT) family are responsible for the exchange of a guanine residue in archaeal tRNAs with a preQ0 base (7-cyano-7-deazaguanine), which constitutes the initial step in archaeosine biosynthesis. Archaeosine is a modified RNA base specific to archaea (7-formamidino-7deazaguanosine), found at position 15 in tRNAs. It has been shown that the PUA domain of archaeosine TGT is not required for its specificity for position 15.
Feature 1:RNA binding site [nucleic acid binding site]
Evidence:
Comment:many PUA domains have been shown to bind RNA, and although detailed interactions vary, they all seem to share a common surface patch that is associated with binding
Structure:1J2B: PUA domain of Pyrococcus horikoshii archaeosine tRNA-guanine transglycosylase binds lambda-form tRNA (Val), contacts at 4 A
Jiyao W et al.(2023). "The conserved domain database in 2023.",