2WIY,2WIV,4EP6


Conserved Protein Domain Family
CYP_XplA

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cd20619: CYP_XplA 
Click on image for an interactive view with Cn3D
cytochrome P450 XplA
XplA is a cytochrome P450 that was found to mediate the microbial metabolism of the military explosive, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). XplA has an unusual structural organization comprising a heme domain that is fused to its flavodoxin redox partner. XplA, along with its partner reductase XplB, are plasmid encoded and the xplA gene has now been found in divergent genera across the globe with near sequence identity. It has only been detected at explosive-contaminated sites, suggesting rapid dissemination of this novel catabolic activity, possibly within a 50-year period since the introduction of RDX into the environment. XplA belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
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PSSM-Id: 410712
Aligned: 4 rows
Threshold Bit Score: 305.509
Created: 16-Aug-2017
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heme bindingchemical
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:heme binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                   ##      #   #                     
2WIY_A     33 HPVHKLEdGTYLVSRYADVSHFAKLPimsvepgwadAGPWAVASDTALGSDPPHHTVLRRQTNKWFtPKLVDGWVRTTRE 112 Rhodococcus
2WIV_A     33 HPVHKLEdGTYLVSRYADVSHFAKLPimsvepgwadAGPWAVASDTALGSDPPHHTVLRRQTNKWFtPKLVDGWVRTTRE 112 Rhodococcus
4EP6_A     31 HPVHKLEdGTYLVSRYADVSHFAKLPimsvepgwadAGPWAVASDTALGSDPPHHTVLRRQTNKWFtPKLVDGWVRTTRE 110 Rhodococcus rhodoc...
CAG74973   44 HPVYRMEnGEIVLTRYHDVMTWLKAPlgis---nfgNGPWNNFDNTVLNCDPPEHTTLRRHSNKWFtPKLVNQYVTIATE 120 Erwinia carotovora...
Feature 1                                  #                                                  
2WIY_A    113 LVGDLLDGVEagqviearrdLAVVPTHVTMARVLqlpedDADAVMEAMFEAMLMQsaepadgdvDRAAVAFGYLSARVAE 192 Rhodococcus
2WIV_A    113 LVGDLLDGVEagqviearrdLAVVPTHVTMARVLqlpedDADAVMEAMFEAMLMQsaepadgdvDRAAVAFGYLSARVAE 192 Rhodococcus
4EP6_A    111 LVGDLLDGVEagqviearrdLAVVPTHVTMARVLqlpedDADAVMEAMFEAMLMQsaepadgdvDRAAVAFGYLSARVAE 190 Rhodococcus rhodoc...
CAG74973  121 LAENALDRYSdgsvmdafyeLAVVPPHATMCRALgvpedDAGLIYRHFLTCTDALghgvgrddtEKASQSFDYLFERCAH 200 Erwinia carotovora...
Feature 1                                             #  ##  ##                               
2WIY_A    193 MLEDKRVNPgdglads---lldaaraGEITESEAIATILVFYAVGHMAIGYLIASGIELFARRpeVFTAFrndesaRAAI 269 Rhodococcus
2WIV_A    193 MLEDKRVNPgdglads---lldaaraGEITESEAIATILVFYAVGHMAIGYLIASGIELFARRpeVFTAFrndesaRAAI 269 Rhodococcus
4EP6_A    191 MLEDKRVNPgdglads---lldaaraGEITESEAIATILVFYAVGHMAIGYLIASGIELFARRpeVFTAFrndesaRAAI 267 Rhodococcus rhodoc...
CAG74973  201 YIKEKRSNPnvgpqglvddflkladeGKLTERAVLETMVLFYGSGSPNPATVIASGLNHFAREpeTFELYrtqpeeRNAI 280 Erwinia carotovora...
Feature 1              ##  # #                      #                            ###  ## ###  
2WIY_A    270 INEMVRMDPPQLSFLRFPTEDVEIggVLIEAGSPIRFMIGAANRDpevfddPDVFDHTRPpaasrNLSFGLGPHSCAGQI 349 Rhodococcus
2WIV_A    270 INEMVRMDPPQLSFLRFPTEDVEIggVLIEAGSPIRFMIGAANRDpevfddPDVFDHTRPpaasrNLSFGLGPHSCAGQI 349 Rhodococcus
4EP6_A    268 INEMVRMDPPQLSFLRFPTEDVEIggVLIEAGSPIRFMIGAANRDpevfddPDVFDHTRPpaasrNLSFGLGPHSCAGQI 347 Rhodococcus rhodoc...
CAG74973  281 INELTRLYPAEISMIRYATEDTEIdgIPVTKGTPVRAVIAAANRDpeffenPHEFNHKRPpetsmNLTFGVGHHACAGQL 360 Erwinia carotovora...
Feature 1     ##                                       
2WIY_A    350 ISRAEATTVFAVLAERYErieLAEEPTVAHndfarRYRKLP 390 Rhodococcus
2WIV_A    350 ISRAEATTVFAVLAERYErieLAEEPTVAHndfarRYRKLP 390 Rhodococcus
4EP6_A    348 ISRAEATTVFAVLAERYErieLAEEPTVAHndfarRYRKLP 388 Rhodococcus rhodochrous
CAG74973  361 ISRSAVRSVFDAVAKKATriqIAGEAGIAHtdrvrGYLSLP 401 Erwinia carotovora subsp. atroseptica SCRI1043

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