2B3R


Conserved Protein Domain Family
C2B_PI3K_class_II

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cd08381: C2B_PI3K_class_II 
Click on image for an interactive view with Cn3D
C2 domain second repeat present in class II phosphatidylinositol 3-kinases (PI3Ks)
There are 3 classes of PI3Ks based on structure, regulation, and specificity. All classes contain a N-terminal C2 domain, a PIK domain, and a kinase catalytic domain. Unlike class I and class III, class II PI3Ks have additionally a PX domain and a C-terminal C2 domain containing a nuclear localization signal both of which bind phospholipids though in a slightly different fashion. PI3Ks (AKA phosphatidylinositol (PtdIns) 3-kinases) regulate cell processes such as cell growth, differentiation, proliferation, and motility. PI3Ks work on phosphorylation of phosphatidylinositol, phosphatidylinositide (4)P (PtdIns (4)P),2 or PtdIns(4,5)P2. Specifically they phosphorylate the D3 hydroxyl group of phosphoinositol lipids on the inositol ring. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-I topology.
Statistics
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PSSM-Id: 176027
View PSSM: cd08381
Aligned: 19 rows
Threshold Bit Score: 160.921
Threshold Setting Gi: 288558821
Created: 14-Jul-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
nuclear
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:nuclear localization signal
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                             ###########                 
2B3R_A          8 SGAVKLSVSYR--NGTLFIMVMHIKDLVTED-GADPNPYVKTYLLPDTHKTSKRKTKISRKTRNPTFNEMLVYSgysKET 84   house mouse
NP_510529    1473 QCQIYLKIEYNsvKETLSVFIGHAKYLALLQnNQQPDPYVKTYVRPDLRNQSKQKTQVVRGTRHPTFNQDLNYTefpIEI 1552 nematode
EEC17358     1511 VGRIKLSIVYK--AGSLSVMVMHAENLSCER-KSSPDPYVKTYLLPDWDKSTKKKTKVVFKNSHPSFMELLVYNf-pLAV 1586 black-legged ...
NP_524028    1750 IGQIRLSLQYQ--RGVLTVMIHHAKGLPMLQgGQEPNTYVKCYLKPDPKKETKRKTKVVRKTCVPSFMETLEYRm-pLNI 1826 fruit fly
XP_001638040 1184 SGQIKLSIRHE--RGALRVMVMHAKDLSSRNsSTAGDPYVKLYLLPDPSKTTKLKTKIAKKSQNPTYNETLVYSnlsAAD 1261 starlet sea a...
XP_002168892 1185 GGKIKLSLVYK--NDAFHILVSHVKELIPRRiEEVIDCYVKCYLLPDPMKCTKRKTKICRRSLNPTFNDILVYDflpLEL 1262 green hydra
XP_002590193 1160 GGSVKLSIHYK--KNGLFIMVMHAKDLQSQD-GEYPNPYVKTYLLPDPAKLTKQKTKIAKKSLNPTYNEMLVYNm-pLAD 1235 Florida lancelet
XP_002645914 1476 DCQVFLKLQHNsvKNTLNVFVGHVKNLPLLQtGQPPDPYVKMYIRPNLQNFPKKKTNIVRDTQHPTFNQEFTYEgfpADM 1555 Caenorhabditi...
XP_686809     961 CRGIQLFLSYK--DQKLSVLIKHLKNIRLSN-GSCPDAYVVTRLRPDPHEKTKRKTKAVRNNDHPTFNELIEYRn--VLK 1035 zebrafish
XP_416412    1300 TPGVQLVISYE--GTHLTVMLKHMRNIRLPD-GSAPSAHAEFYLLPDPGEVNRRKTRTVPKTTNPTYNEIVVYNk--VMQ 1374 chicken
Feature 1                                                               
2B3R_A         85 LRqrELQLSVLSAESLr--ENFFLGGITLplkdfn-------lsketVKWYQLT 129  house mouse
NP_510529    1553 LStrVLEVSIWNNGGYl--VKHKMYMLCIpllkvkklaesrkncrtlEGWFNCE 1604 nematode
EEC17358     1587 VRsrMLEVSAWDHDRVq--ENEFLGAAIIelsrmd-------lstetTHWYHLN 1631 black-legged tick
NP_524028    1827 IQerRLQVTVWSHDTLq--ENELLGGFDMdlskyd-------lrqelVDWYRLG 1871 fruit fly
XP_001638040 1262 LKtrHLQVTVWDHDRVg--ENDFMGGVVInladmd-------ssiniTNWYPLK 1306 starlet sea anemone
XP_002168892 1263 LKkrLLQVSVWEHDPLn--KNEFLGGVDIylnnfn-------lmnpvSDWFTLT 1307 green hydra
XP_002590193 1236 VQrrTLQLTVWSSESVr--ENCFLGGTSIrlgeld-------lkqeaVQWYRLG 1280 Florida lancelet
XP_002645914 1556 LNsrVLEVDIWNSSGIl--EKHKMFSMCIpliklyqakesrksqrvvEGWFQCY 1607 Caenorhabditis briggsae
XP_686809    1036 LHglVLEVTVKSRKAFvaaTNVVLGERVLe----------------qEKWFPLG 1073 zebrafish
XP_416412    1375 LEghILKLVVKSKGTFvgaVNIQLSRVQLn----------------eEKWYPLG 1412 chicken

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