2GFG


Conserved Protein Domain Family
CYTH-like_Pase_1

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cd07762: CYTH-like_Pase_1 
Click on image for an interactive view with Cn3D
Uncharacterized subgroup 1 of the CYTH-like superfamily
Enzymes belonging to the CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) superfamily hydrolyze triphosphate-containing substrates, require metal cations as cofactors, and have a unique active site located at the center of an eight-stranded antiparallel beta barrel tunnel (the triphosphate tunnel). The name CYTH originated from the gene designation for bacterial class IV adenylyl cyclases (CyaB) and from thiamine triphosphatase. Class IV adenylate cyclases catalyze the conversion of ATP to 3',5'-cyclic AMP (cAMP) and PPi. Thiamine triphosphatase is a soluble cytosolic enzyme which converts thiamine triphosphate to thiamine diphosphate. This domain superfamily also contains RNA triphosphatases, membrane-associated polyphosphate polymerases, tripolyphosphatases, nucleoside triphosphatases, nucleoside tetraphosphatases and other proteins with unknown functions. Proteins of this subgroup are of bacterial origin and have not been characterized.
Statistics
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PSSM-Id: 143627
View PSSM: cd07762
Aligned: 37 rows
Threshold Bit Score: 142.335
Threshold Setting Gi: 161702095
Created: 28-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on similar proteins with structure, and on NMR titration experiments of mouse Thiamine triphosphatase, and on mutation analysis of Clostridium thermocellum TTM.
  • Comment:This active site includes the metal binding and triphosphate binding sites.
  • Structure:2GFG_A; Bacillus halodurans Bh2851 bound with hydroperoxy-dihydroxy-oxidanium, contacts determined at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         # # #                            #               # # #        #               #
2GFG_A         6 IEIERKTLVSkETFKRLISQLhigegDFKLQRNHYFETddFQLKKQSSALRIREKeaIFTFTLKQPHp-------aGLLE 78  Bacillus halodu...
ZP_04566330    5 LEIEFKVLINkEIYQQIYNDHhi--dHQYSQTNYYLIH--PKLTELKYMLRIRQKqeNYELTLKQPQs-------yGNLE 73  Mollicutes bact...
ZP_03681658    2 LEIEYKMLLTeEVFHQLMNDFt---gHTYSQTNYYLTS--IELGALRYSLRIREKegYYEMTLKTPAa-------nGRME 69  Catenibacterium...
ZP_03635582   38 LEIEYKMLVSeQEFNQLRDLTgv--dQVLVQSNVYYDCt-PSSTLKHIAMRIRDVqgKHWFTMKIPQa-------eGVRE 107 Holdemania fili...
ZP_02077039    5 IEKEYKLLVTkEQFEQLVSLYep--iFFHKQVNTYYDTtdHRIADLHGAMRIREIdgHFIFTLKLWKk-------qQLYE 75  Eubacterium dol...
ZP_03489305    5 IERELKLLINkQVYDNLMNSYdf--hKSIIQTNTYFDTanQDVKKQHGAVRIRTIenKKIFTLKIKKde------yAHYE 76  Eubacterium bif...
ZP_04440435    5 REQEFKTCLSkNAFLALLNYFdf--ePAFSQMNTYYDTadGAVKAARLGLRIRQFadHAEQTLKVPNest----qrDLEE 78  Lactobacillus r...
YP_618673      5 REIEAKVALDqASYEAIVAAYpv--kSDFKQSNHYFDTpdKQLKEDHSALRIRCFqdRAEETIKVPVkklq-etyhESLE 81  Lactobacillus d...
NP_964677      5 LEIESKTLLDkETYEKMRAAFta--kSDFIQKNYYFDTpdFDLKNSDASLRIRILvdHAEQTIKVKEtnpkekkysERIE 82  Lactobacillus j...
YP_001577130   5 REIEAKTLLHrETYQKIVNDFpv--kSDFVQENYYFDTpdKLLKKHHISLRIRIYsdHAEQTMKVPDpnpvqrnyhEVIE 82  Lactobacillus h...
Feature 1                                                              # #             #         
2GFG_A        79 TNQTLs--kQEAKLALesahf-----psgeVXDALRDLsip--isqLKHIGTLSTSRAEISYe-qGILCLDHSSYl-gIE 147 Bacillus halodu...
ZP_04566330   74 TNLKId--kITKEKIIthdf------vtneIFDLLKPLgld--stmFKTDCVLTTTRCEIKTt-dGLICLDQSLYn-gIT 141 Mollicutes bact...
ZP_03681658   70 HNLEIt---KEDLIMIqngqm-----pdneITCLLKDKgfd--lslIKQSYSLTTIRRDVPFd-sGMLSLDENHYn-gIT 137 Catenibacterium...
ZP_03635582  108 MEMELp---ENSPEALdn----------peIRALFDELglt---lpVHVCGQMITHRHLRVYp-lGELCLDHSLIn-gRS 169 Holdemania fili...
ZP_02077039   76 YECEVknnsIEALQNEqi-----------vMLLKEHGIyg-----eMTVLTSLTTYRGEVISn-dAILCFDHSLYn-gIE 137 Eubacterium dol...
ZP_03489305   77 FEKEIs--tENINEIEdp-----------eILNWFNQYqip---knLHPTATFTTLRNVYEFe-nGELCLDKTTFk-nHI 138 Eubacterium bif...
ZP_04440435   79 ITDPLs--lVEAQKLIeqgel-----ksgqVTQALAARgidp-atvAPQTQAKTTRRLAHLPa--GLLTLDQTFYidqTS 148 Lactobacillus r...
YP_618673     82 INDDLt--lPEAEDFLkkgrp----sltgnVGQYLLEHypdk-asqLTLFSWSKTRRILLDGpkgCELTLDQTSYpdgYQ 154 Lactobacillus d...
NP_964677     83 INDLLs--vAQAEQMTqssyegdfflfggdVGDYLQKHysneaihsLKLISWSQTRRILANGpenCELTLDLTEFpdgYY 160 Lactobacillus j...
YP_001577130  83 INDNLt--hAQAKELVakkhf----afhgnIGQYLTAHfake-qdaLSLFTWSKTRRILMNGpqdCELTLDATSYpdgYE 155 Lactobacillus h...
Feature 1            # #                               #     
2GFG_A       148 DYEIEFEGts-eeHATVTFQEILKTFSISQVPt---eNKIQRFF 187 Bacillus halodurans
ZP_04566330  142 DYELEYEVfd-yhHGKQVFLDFISQYNLKYSRnc--pSKVKRLM 182 Mollicutes bacterium D7
ZP_03681658  138 DYELEFEVnd-eeAGLKQFKELCEIYHLQYKEnc--aSKIVRVL 178 Catenibacterium mitsuokai DSM 15897
ZP_03635582  170 DYEIEFEItgdpqAGKAFFLDLLNQAGLTYREnk--rSKYARCV 211 Holdemania filiformis DSM 12042
ZP_02077039  138 DFEIEYEYrr-ehDGRRAFQTILAPLHIQYEKnc--iSKIQRAL 178 Eubacterium dolichum DSM 3991
ZP_03489305  139 DYEIEYEYts-dhDGIQFFNSILEQYGLKWQKnc--pSKIARAM 179 Eubacterium biforme DSM 3989
ZP_04440435  149 DFELEMEYqn-laVAQKFYDDLLRHFHIKKQPv---iNKVQRAY 188 Lactobacillus rhamnosus LMS2-1
YP_618673    155 DWEMEIENds-paAIKEALAEIKTRFALPVKSan-nqSKIARAS 196 Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
NP_964677    161 DFELEIENdd-pdIIKKVLSELEKQFNFTVNKentnqSKVQRAW 203 Lactobacillus johnsonii NCC 533
YP_001577130 156 DFELEIENdd-pvLITKVLTILESTYNFKQDSsntnkSKIFRAS 198 Lactobacillus helveticus DPC 4571

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